Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms
Pseudomonas aeruginosa is a pathogenic bacterium that can be considered a high risk to human health due to its remarkable capacity to resist antibiotics, either intrinsically or following the acquisition of resistance genes. P. aeruginosa has been considered a major threat to human health as all the...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24032/ http://journalarticle.ukm.my/24032/1/SEE%206.pdf |
| Summary: | Pseudomonas aeruginosa is a pathogenic bacterium that can be considered a high risk to human health due to its remarkable capacity to resist antibiotics, either intrinsically or following the acquisition of resistance genes. P. aeruginosa has been considered a major threat to human health as all the known remedies seem ineffective. Bacteriophages as a natural killer of bacteria can offer alternative therapy for antibiotics. The study aimed to isolate and characterize a lytic bacteriophage against P. aeruginosa (clinically resistant strain/superbug) and to investigate its lytic potential to control bacterial planktonic cells and their biofilms on stainless steel surfaces. A lytic bacteriophage known as MAC-1 was isolated from wastewater against the selected P. aeruginosa-2750 strain. Its host range was moderate and it only infected six isolates. Phage MAC-1 was characterized through transmission electron microscopy and was classified to the Siphoviridae family. It has good heat and pH stability. Its latent time was 24 min with a burst size of about 410 virions per cell. The phage MAC-1 efficacy was determined against P. aeruginosa planktonic cells and biofilms on metallic surfaces. Isolated phage MAC-1 demonstrated promising activity against bacterial planktonic cells as well as in reducing bacterial biofilm biomass formed in 96-well and on stainless steel plates. However, a phage cocktail may be used to avoid resistance and ensure complete eradication of bacterial biofilms. |
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