Modulation of human bone marrow mesenchymal stem cells (BMMSC) by nigella sativa and trigona honey: an in vitro study
Maintenance of bone marrow mesenchymal stem cells (BMMSC) numbers is crucial for tissue repair and regeneration since adult stem cells are often limited in number, expansion capacity and lifespan. It is believed that certain types of foods are beneficial in the preservation and stimulation of stem c...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/23912/ http://journalarticle.ukm.my/23912/1/SE%205.pdf |
| _version_ | 1848815966249549824 |
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| author | Masniza Muhamad Lasim, Nurul Farihah Samsudin, Zetty Nadia Mohd Zain, Hayati Abdul Rahman, Nur Syahrina Rahim, Asral Wirda Ahmad Asnawi, Nur Fariha Mohd Manzor, |
| author_facet | Masniza Muhamad Lasim, Nurul Farihah Samsudin, Zetty Nadia Mohd Zain, Hayati Abdul Rahman, Nur Syahrina Rahim, Asral Wirda Ahmad Asnawi, Nur Fariha Mohd Manzor, |
| author_sort | Masniza Muhamad Lasim, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Maintenance of bone marrow mesenchymal stem cells (BMMSC) numbers is crucial for tissue repair and regeneration since adult stem cells are often limited in number, expansion capacity and lifespan. It is believed that certain types of foods are beneficial in the preservation and stimulation of stem cells throughout life. Black seeds and stingless bee honey are among the widely investigated functional food with general health promoting benefit. We aim to explore the proliferative, anti-apoptosis and anti-senescence effect of Nigella sativa and Trigona honey on BMMSC. Cell proliferation was evaluated using MTT assay. We performed flow cytometric analysis to verify stem cell surface markers while quantitative PCR was done to measure the relative expression of target genes. Results showed favourable concentration dependent enhancement of cell proliferation by 1 to 10 ug/mL Nigella sativa and 0.097% to 0.195% Trigona honey. Observation on BMMSC’s morphology and surface markers expression revealed no alteration on BMMSC stemness properties. In addition, gene expression analysis supported that there was a significant (p<0.05) increase in proliferation related gene, β-Catenin, and lower expression of apoptotic- and senescence-related gene, BAX and p21, respectively. These preliminary findings suggest a promising effect of Nigella sativa and Trigona honey on stem cell proliferation. |
| first_indexed | 2025-11-15T00:58:22Z |
| format | Article |
| id | oai:generic.eprints.org:23912 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:58:22Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:239122024-08-05T06:47:13Z http://journalarticle.ukm.my/23912/ Modulation of human bone marrow mesenchymal stem cells (BMMSC) by nigella sativa and trigona honey: an in vitro study Masniza Muhamad Lasim, Nurul Farihah Samsudin, Zetty Nadia Mohd Zain, Hayati Abdul Rahman, Nur Syahrina Rahim, Asral Wirda Ahmad Asnawi, Nur Fariha Mohd Manzor, Maintenance of bone marrow mesenchymal stem cells (BMMSC) numbers is crucial for tissue repair and regeneration since adult stem cells are often limited in number, expansion capacity and lifespan. It is believed that certain types of foods are beneficial in the preservation and stimulation of stem cells throughout life. Black seeds and stingless bee honey are among the widely investigated functional food with general health promoting benefit. We aim to explore the proliferative, anti-apoptosis and anti-senescence effect of Nigella sativa and Trigona honey on BMMSC. Cell proliferation was evaluated using MTT assay. We performed flow cytometric analysis to verify stem cell surface markers while quantitative PCR was done to measure the relative expression of target genes. Results showed favourable concentration dependent enhancement of cell proliferation by 1 to 10 ug/mL Nigella sativa and 0.097% to 0.195% Trigona honey. Observation on BMMSC’s morphology and surface markers expression revealed no alteration on BMMSC stemness properties. In addition, gene expression analysis supported that there was a significant (p<0.05) increase in proliferation related gene, β-Catenin, and lower expression of apoptotic- and senescence-related gene, BAX and p21, respectively. These preliminary findings suggest a promising effect of Nigella sativa and Trigona honey on stem cell proliferation. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23912/1/SE%205.pdf Masniza Muhamad Lasim, and Nurul Farihah Samsudin, and Zetty Nadia Mohd Zain, and Hayati Abdul Rahman, and Nur Syahrina Rahim, and Asral Wirda Ahmad Asnawi, and Nur Fariha Mohd Manzor, (2024) Modulation of human bone marrow mesenchymal stem cells (BMMSC) by nigella sativa and trigona honey: an in vitro study. Sains Malaysiana, 53 (4). pp. 781-794. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num4_2024/contentsVol53num4_2024.html |
| spellingShingle | Masniza Muhamad Lasim, Nurul Farihah Samsudin, Zetty Nadia Mohd Zain, Hayati Abdul Rahman, Nur Syahrina Rahim, Asral Wirda Ahmad Asnawi, Nur Fariha Mohd Manzor, Modulation of human bone marrow mesenchymal stem cells (BMMSC) by nigella sativa and trigona honey: an in vitro study |
| title | Modulation of human bone marrow mesenchymal stem cells (BMMSC) by nigella sativa and trigona honey: an in vitro study |
| title_full | Modulation of human bone marrow mesenchymal stem cells (BMMSC) by nigella sativa and trigona honey: an in vitro study |
| title_fullStr | Modulation of human bone marrow mesenchymal stem cells (BMMSC) by nigella sativa and trigona honey: an in vitro study |
| title_full_unstemmed | Modulation of human bone marrow mesenchymal stem cells (BMMSC) by nigella sativa and trigona honey: an in vitro study |
| title_short | Modulation of human bone marrow mesenchymal stem cells (BMMSC) by nigella sativa and trigona honey: an in vitro study |
| title_sort | modulation of human bone marrow mesenchymal stem cells (bmmsc) by nigella sativa and trigona honey: an in vitro study |
| url | http://journalarticle.ukm.my/23912/ http://journalarticle.ukm.my/23912/ http://journalarticle.ukm.my/23912/1/SE%205.pdf |