The phytochemical components of Kelantan grown Annona muricata leaves and its anti-proliferative properties on MCF-7 breast cancer cells
Annona muricata leaves exhibit anti-cancer properties against breast cancer. In this study, the phytochemical components, anti-proliferative activity, and cytotoxicity of Kelantan (Malaysia) grown Annona muricata leaves were evaluated using MCF-7 breast cancer cell line. The preparation of Annona mu...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2023
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| Online Access: | http://journalarticle.ukm.my/23305/ http://journalarticle.ukm.my/23305/1/SS%205.pdf |
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| author | Norzila Ismail, Harishini Rajaratinam, Khairunnisa Che Ghazali, Siti Farhanah Mohd Salleh, Wan Suriyani Faliq Adeeba Wan Ibrahim, Maya Mazuwin Yahya, Wan Zainira Wan Zain, |
| author_facet | Norzila Ismail, Harishini Rajaratinam, Khairunnisa Che Ghazali, Siti Farhanah Mohd Salleh, Wan Suriyani Faliq Adeeba Wan Ibrahim, Maya Mazuwin Yahya, Wan Zainira Wan Zain, |
| author_sort | Norzila Ismail, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Annona muricata leaves exhibit anti-cancer properties against breast cancer. In this study, the phytochemical components, anti-proliferative activity, and cytotoxicity of Kelantan (Malaysia) grown Annona muricata leaves were evaluated using MCF-7 breast cancer cell line. The preparation of Annona muricata leaves extracts was performed with various organic solvents (ethyl acetate, n-hexane, methanol, and aqueous) using Soxhlet method. Each extract was analysed using gas chromatography-mass spectrometry (GCMS) for phytochemical analysis and characterised with Wiley and NIST library searchers. The effects of all extracts on cell proliferation were analysed using MTT assay. The anti-proliferative activity was determined by evaluating the IC50 value. Based on the lowest IC50 value, further experiments were done to observe the apoptosis effect using the Annexin V-FITC technique. While for cell cycle analysis, Cycletest Plus DNA Reagent Kit was used. Both were further determined using flow cytometry analysis. The ethyl acetate-based Annona muricata extract contained terpenoids, phenolic compounds, and alkaloids. Ethyl acetate extract of Annona muricata exhibited a significant cytotoxic effect on MCF-7 breast cancer cells. The most potent IC50 value obtained was 21.8 ± 3.85 µg/mL. The changes in morphology were more profound after 72 h of treatment with ethyl acetate extract of Annona muricata, including cell shrinkage. Flow cytometry analysis showed that it was induced in early and late apoptosis in vitro and arrest at G0 /G1 cell cycle phase in time-dependant manners. In short, ethyl acetate extract of Annona muricata exhibited a range of phytochemical components, anti-proliferative activity and cytotoxic effect on MCF-7 breast cancer cells. |
| first_indexed | 2025-11-15T00:55:55Z |
| format | Article |
| id | oai:generic.eprints.org:23305 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:55:55Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:233052024-04-01T05:37:59Z http://journalarticle.ukm.my/23305/ The phytochemical components of Kelantan grown Annona muricata leaves and its anti-proliferative properties on MCF-7 breast cancer cells Norzila Ismail, Harishini Rajaratinam, Khairunnisa Che Ghazali, Siti Farhanah Mohd Salleh, Wan Suriyani Faliq Adeeba Wan Ibrahim, Maya Mazuwin Yahya, Wan Zainira Wan Zain, Annona muricata leaves exhibit anti-cancer properties against breast cancer. In this study, the phytochemical components, anti-proliferative activity, and cytotoxicity of Kelantan (Malaysia) grown Annona muricata leaves were evaluated using MCF-7 breast cancer cell line. The preparation of Annona muricata leaves extracts was performed with various organic solvents (ethyl acetate, n-hexane, methanol, and aqueous) using Soxhlet method. Each extract was analysed using gas chromatography-mass spectrometry (GCMS) for phytochemical analysis and characterised with Wiley and NIST library searchers. The effects of all extracts on cell proliferation were analysed using MTT assay. The anti-proliferative activity was determined by evaluating the IC50 value. Based on the lowest IC50 value, further experiments were done to observe the apoptosis effect using the Annexin V-FITC technique. While for cell cycle analysis, Cycletest Plus DNA Reagent Kit was used. Both were further determined using flow cytometry analysis. The ethyl acetate-based Annona muricata extract contained terpenoids, phenolic compounds, and alkaloids. Ethyl acetate extract of Annona muricata exhibited a significant cytotoxic effect on MCF-7 breast cancer cells. The most potent IC50 value obtained was 21.8 ± 3.85 µg/mL. The changes in morphology were more profound after 72 h of treatment with ethyl acetate extract of Annona muricata, including cell shrinkage. Flow cytometry analysis showed that it was induced in early and late apoptosis in vitro and arrest at G0 /G1 cell cycle phase in time-dependant manners. In short, ethyl acetate extract of Annona muricata exhibited a range of phytochemical components, anti-proliferative activity and cytotoxic effect on MCF-7 breast cancer cells. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23305/1/SS%205.pdf Norzila Ismail, and Harishini Rajaratinam, and Khairunnisa Che Ghazali, and Siti Farhanah Mohd Salleh, and Wan Suriyani Faliq Adeeba Wan Ibrahim, and Maya Mazuwin Yahya, and Wan Zainira Wan Zain, (2023) The phytochemical components of Kelantan grown Annona muricata leaves and its anti-proliferative properties on MCF-7 breast cancer cells. Sains Malaysiana, 52 (10). pp. 2785-2801. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol52num10_2023/contentsVol52num10_2023.html |
| spellingShingle | Norzila Ismail, Harishini Rajaratinam, Khairunnisa Che Ghazali, Siti Farhanah Mohd Salleh, Wan Suriyani Faliq Adeeba Wan Ibrahim, Maya Mazuwin Yahya, Wan Zainira Wan Zain, The phytochemical components of Kelantan grown Annona muricata leaves and its anti-proliferative properties on MCF-7 breast cancer cells |
| title | The phytochemical components of Kelantan grown Annona muricata leaves and its anti-proliferative properties on MCF-7 breast cancer cells |
| title_full | The phytochemical components of Kelantan grown Annona muricata leaves and its anti-proliferative properties on MCF-7 breast cancer cells |
| title_fullStr | The phytochemical components of Kelantan grown Annona muricata leaves and its anti-proliferative properties on MCF-7 breast cancer cells |
| title_full_unstemmed | The phytochemical components of Kelantan grown Annona muricata leaves and its anti-proliferative properties on MCF-7 breast cancer cells |
| title_short | The phytochemical components of Kelantan grown Annona muricata leaves and its anti-proliferative properties on MCF-7 breast cancer cells |
| title_sort | phytochemical components of kelantan grown annona muricata leaves and its anti-proliferative properties on mcf-7 breast cancer cells |
| url | http://journalarticle.ukm.my/23305/ http://journalarticle.ukm.my/23305/ http://journalarticle.ukm.my/23305/1/SS%205.pdf |