Elucidating the lignocellulose digestion mechanism coptotermes curvignathus based on carbohydrate-active enzymes profile using the meta-transcriptomic approach
Termites are efficient lignocellulose decomposers that thrive on woody materials and contribute to carbon mineralization in both tropical and subtropical regions. Due to hydrolytic stability and crosslinking between the polysaccharides (cellulose & hemicellulose) and the lignin via ester and eth...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2023
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| Online Access: | http://journalarticle.ukm.my/23150/ http://journalarticle.ukm.my/23150/1/ML%2020.pdf |
| _version_ | 1848815773244456960 |
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| author | Pik, Kheng Hoe Jie, Hung King Kian, Huat Ong Choon, Fah Bong Nor Muhammad Mahadi, |
| author_facet | Pik, Kheng Hoe Jie, Hung King Kian, Huat Ong Choon, Fah Bong Nor Muhammad Mahadi, |
| author_sort | Pik, Kheng Hoe |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Termites are efficient lignocellulose decomposers that thrive on woody materials and contribute to carbon mineralization in both tropical and subtropical regions. Due to hydrolytic stability and crosslinking between the polysaccharides (cellulose & hemicellulose) and the lignin via ester and ether linkages, termites would require a large variety of enzymes to degrade lignocellulose. Coptotermes curvignathus, an endemic species of termite from Southeast Asia, has been classified as an urban pest in the region and is known as the largest and most aggressive among the oriental Coptotermes spp. Its Carbohydrate-Active enzymes (CAZymes) are the main interest of this study. RNA of C. curvignathus was extracted and sequenced using Illumina Hiseq 2000 sequencing platform, and de novo assembled with Trinity pipeline. There were 101 CAZymes families in C. curvignathus digestome. CAZymes break down complex carbohydrates and glycoconjugates for a large body of biological roles and perform their function, usually with high specificity. Enzymes coding for glycosyl hydrolase (GH) families had the highest transcript abundance, accounting for about 93% of the total CAZymes reads. This was followed by CBM (≈1%), GT family (≈4%), CE family (<1 %) AA family (<2 %) and PL family (<1 %). Due to the carbohydrate diversity exceeding the number of protein folds, CAZymes have evolved from a limited number of progenitors by acquiring novel specificities at substrate and product levels. Such a dizzying array of substrates and enzymes makes C. curvignathus a high-performance lignocellulose degrader |
| first_indexed | 2025-11-15T00:55:18Z |
| format | Article |
| id | oai:generic.eprints.org:23150 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:55:18Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:231502024-03-06T05:00:39Z http://journalarticle.ukm.my/23150/ Elucidating the lignocellulose digestion mechanism coptotermes curvignathus based on carbohydrate-active enzymes profile using the meta-transcriptomic approach Pik, Kheng Hoe Jie, Hung King Kian, Huat Ong Choon, Fah Bong Nor Muhammad Mahadi, Termites are efficient lignocellulose decomposers that thrive on woody materials and contribute to carbon mineralization in both tropical and subtropical regions. Due to hydrolytic stability and crosslinking between the polysaccharides (cellulose & hemicellulose) and the lignin via ester and ether linkages, termites would require a large variety of enzymes to degrade lignocellulose. Coptotermes curvignathus, an endemic species of termite from Southeast Asia, has been classified as an urban pest in the region and is known as the largest and most aggressive among the oriental Coptotermes spp. Its Carbohydrate-Active enzymes (CAZymes) are the main interest of this study. RNA of C. curvignathus was extracted and sequenced using Illumina Hiseq 2000 sequencing platform, and de novo assembled with Trinity pipeline. There were 101 CAZymes families in C. curvignathus digestome. CAZymes break down complex carbohydrates and glycoconjugates for a large body of biological roles and perform their function, usually with high specificity. Enzymes coding for glycosyl hydrolase (GH) families had the highest transcript abundance, accounting for about 93% of the total CAZymes reads. This was followed by CBM (≈1%), GT family (≈4%), CE family (<1 %) AA family (<2 %) and PL family (<1 %). Due to the carbohydrate diversity exceeding the number of protein folds, CAZymes have evolved from a limited number of progenitors by acquiring novel specificities at substrate and product levels. Such a dizzying array of substrates and enzymes makes C. curvignathus a high-performance lignocellulose degrader Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23150/1/ML%2020.pdf Pik, Kheng Hoe and Jie, Hung King and Kian, Huat Ong and Choon, Fah Bong and Nor Muhammad Mahadi, (2023) Elucidating the lignocellulose digestion mechanism coptotermes curvignathus based on carbohydrate-active enzymes profile using the meta-transcriptomic approach. Malaysian Applied Biology, 52 (5). pp. 177-186. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/56 |
| spellingShingle | Pik, Kheng Hoe Jie, Hung King Kian, Huat Ong Choon, Fah Bong Nor Muhammad Mahadi, Elucidating the lignocellulose digestion mechanism coptotermes curvignathus based on carbohydrate-active enzymes profile using the meta-transcriptomic approach |
| title | Elucidating the lignocellulose digestion mechanism coptotermes curvignathus based on carbohydrate-active enzymes profile using the meta-transcriptomic approach |
| title_full | Elucidating the lignocellulose digestion mechanism coptotermes curvignathus based on carbohydrate-active enzymes profile using the meta-transcriptomic approach |
| title_fullStr | Elucidating the lignocellulose digestion mechanism coptotermes curvignathus based on carbohydrate-active enzymes profile using the meta-transcriptomic approach |
| title_full_unstemmed | Elucidating the lignocellulose digestion mechanism coptotermes curvignathus based on carbohydrate-active enzymes profile using the meta-transcriptomic approach |
| title_short | Elucidating the lignocellulose digestion mechanism coptotermes curvignathus based on carbohydrate-active enzymes profile using the meta-transcriptomic approach |
| title_sort | elucidating the lignocellulose digestion mechanism coptotermes curvignathus based on carbohydrate-active enzymes profile using the meta-transcriptomic approach |
| url | http://journalarticle.ukm.my/23150/ http://journalarticle.ukm.my/23150/ http://journalarticle.ukm.my/23150/1/ML%2020.pdf |