Effects of cobalt-60 gamma on microbial elimination and phytochemical constituents in orthosiphon aristatus (Misai Kucing) (Blume) Miq.

Medicinal plants are used for various purposes, however, the presence of microorganisms in them is the main safety risk. The study aimed to evaluate the effects of gamma irradiation on microbial contaminants and phytochemical constituents of Orthosiphon aristatus (Blume) Miq. The plant was irradiate...

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Main Authors: Syafiqah Mhd Jamal, Hasnah Bahari, Arapoc, Daryl Jesus, Muhammad Akif Adnan, Imran Jazary, Mad Diah, Azmiza Syawani Jasni
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/22569/
http://journalarticle.ukm.my/22569/1/STT%2010.pdf
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author Syafiqah Mhd Jamal,
Hasnah Bahari,
Arapoc, Daryl Jesus
Muhammad Akif Adnan,
Imran Jazary,
Mad Diah,
Azmiza Syawani Jasni,
author_facet Syafiqah Mhd Jamal,
Hasnah Bahari,
Arapoc, Daryl Jesus
Muhammad Akif Adnan,
Imran Jazary,
Mad Diah,
Azmiza Syawani Jasni,
author_sort Syafiqah Mhd Jamal,
building UKM Institutional Repository
collection Online Access
description Medicinal plants are used for various purposes, however, the presence of microorganisms in them is the main safety risk. The study aimed to evaluate the effects of gamma irradiation on microbial contaminants and phytochemical constituents of Orthosiphon aristatus (Blume) Miq. The plant was irradiated using doses of 3, 6, 9, and 12 kGy and the microbial contamination was assessed using phenotypic and genotypic analyses. The qualitative screening using chemical tests was performed to identify the presence of important phytochemical constituents including alkaloids, saponins, flavonoids, tannins, steroids and triterpenes. Results showed that the total microbial counts in O. aristatus were significantly reduced (P < 0.05) following irradiations at 3- and 6 kGy. Pathogenic bacteria were not detected in O. aristatus after irradiation at 6 kGy while the phytochemical constituents were conserved. In conclusion, gamma irradiation has significantly reduced and eliminated microbial contaminants and preserved the phytochemical constituents of O. aristatus. This study highlights the use of a low and specific dose, 6 kGy that is effective to reduce and eliminate microbial contaminants in O. aristatus.
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spelling oai:generic.eprints.org:225692023-11-23T04:40:35Z http://journalarticle.ukm.my/22569/ Effects of cobalt-60 gamma on microbial elimination and phytochemical constituents in orthosiphon aristatus (Misai Kucing) (Blume) Miq. Syafiqah Mhd Jamal, Hasnah Bahari, Arapoc, Daryl Jesus Muhammad Akif Adnan, Imran Jazary, Mad Diah, Azmiza Syawani Jasni, Medicinal plants are used for various purposes, however, the presence of microorganisms in them is the main safety risk. The study aimed to evaluate the effects of gamma irradiation on microbial contaminants and phytochemical constituents of Orthosiphon aristatus (Blume) Miq. The plant was irradiated using doses of 3, 6, 9, and 12 kGy and the microbial contamination was assessed using phenotypic and genotypic analyses. The qualitative screening using chemical tests was performed to identify the presence of important phytochemical constituents including alkaloids, saponins, flavonoids, tannins, steroids and triterpenes. Results showed that the total microbial counts in O. aristatus were significantly reduced (P < 0.05) following irradiations at 3- and 6 kGy. Pathogenic bacteria were not detected in O. aristatus after irradiation at 6 kGy while the phytochemical constituents were conserved. In conclusion, gamma irradiation has significantly reduced and eliminated microbial contaminants and preserved the phytochemical constituents of O. aristatus. This study highlights the use of a low and specific dose, 6 kGy that is effective to reduce and eliminate microbial contaminants in O. aristatus. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22569/1/STT%2010.pdf Syafiqah Mhd Jamal, and Hasnah Bahari, and Arapoc, Daryl Jesus and Muhammad Akif Adnan, and Imran Jazary, and Mad Diah, and Azmiza Syawani Jasni, (2023) Effects of cobalt-60 gamma on microbial elimination and phytochemical constituents in orthosiphon aristatus (Misai Kucing) (Blume) Miq. Sains Malaysiana, 52 (7). pp. 2009-2020. ISSN 0126-6039 https://www.ukm.my/jsm/
spellingShingle Syafiqah Mhd Jamal,
Hasnah Bahari,
Arapoc, Daryl Jesus
Muhammad Akif Adnan,
Imran Jazary,
Mad Diah,
Azmiza Syawani Jasni,
Effects of cobalt-60 gamma on microbial elimination and phytochemical constituents in orthosiphon aristatus (Misai Kucing) (Blume) Miq.
title Effects of cobalt-60 gamma on microbial elimination and phytochemical constituents in orthosiphon aristatus (Misai Kucing) (Blume) Miq.
title_full Effects of cobalt-60 gamma on microbial elimination and phytochemical constituents in orthosiphon aristatus (Misai Kucing) (Blume) Miq.
title_fullStr Effects of cobalt-60 gamma on microbial elimination and phytochemical constituents in orthosiphon aristatus (Misai Kucing) (Blume) Miq.
title_full_unstemmed Effects of cobalt-60 gamma on microbial elimination and phytochemical constituents in orthosiphon aristatus (Misai Kucing) (Blume) Miq.
title_short Effects of cobalt-60 gamma on microbial elimination and phytochemical constituents in orthosiphon aristatus (Misai Kucing) (Blume) Miq.
title_sort effects of cobalt-60 gamma on microbial elimination and phytochemical constituents in orthosiphon aristatus (misai kucing) (blume) miq.
url http://journalarticle.ukm.my/22569/
http://journalarticle.ukm.my/22569/
http://journalarticle.ukm.my/22569/1/STT%2010.pdf