Evaluation of the in-vitro anthelmintic activity of Leucas zeylanica extracts on earthworms

Leucas zeylanica known as Ketumbit has a wide range of medical applications. This fact includes the traditional practice of L. zeylanica for the treatment of worm infections successfully, however to date, the anthelmintic effect has still not been scientifically proven. Therefore, the present study...

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Main Authors: Hemagirri Manisekaran, Muhammad Luqman Selahuddeen, Zaidah Rahmat, Faizuan Abdullah, Abdul Fatah A. Samad
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/22521/
http://journalarticle.ukm.my/22521/1/ME%207.pdf
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author Hemagirri Manisekaran,
Muhammad Luqman Selahuddeen,
Zaidah Rahmat,
Faizuan Abdullah,
Abdul Fatah A. Samad,
author_facet Hemagirri Manisekaran,
Muhammad Luqman Selahuddeen,
Zaidah Rahmat,
Faizuan Abdullah,
Abdul Fatah A. Samad,
author_sort Hemagirri Manisekaran,
building UKM Institutional Repository
collection Online Access
description Leucas zeylanica known as Ketumbit has a wide range of medical applications. This fact includes the traditional practice of L. zeylanica for the treatment of worm infections successfully, however to date, the anthelmintic effect has still not been scientifically proven. Therefore, the present study aimed to evaluate the anthelmintic activity of L. zeylanica. The study began with the extraction of L. zeylanica extracts using methanol, ethanol, and aqueous. Subsequently, experiments were conducted to evaluate the possible in vitro anthelmintic activity of various extracts of L. zeylanica against earthworms. Various concentrations (25, 50 & 100 mg/mL) of extracts were tested and results were expressed in terms of the time of paralysis and time of death of worms. All extracts of the plant exhibited considerable anthelmintic activities in a dose-dependent manner. Of them all, methanolic extract at 100 mg/mL showed the most efficacious anthelmintic activity that was comparable to the reference drug (albendazole, 25 mg/mL). Therefore, we conclude that methanolic extract at the concentration of 100 mg/mL could be considered a candidate for worm treatment apart from the standard medication. This work may provide a framework for further study of L. zeylanica as an alternative treatment for worm infection.
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spelling oai:generic.eprints.org:225212023-11-21T02:10:38Z http://journalarticle.ukm.my/22521/ Evaluation of the in-vitro anthelmintic activity of Leucas zeylanica extracts on earthworms Hemagirri Manisekaran, Muhammad Luqman Selahuddeen, Zaidah Rahmat, Faizuan Abdullah, Abdul Fatah A. Samad, Leucas zeylanica known as Ketumbit has a wide range of medical applications. This fact includes the traditional practice of L. zeylanica for the treatment of worm infections successfully, however to date, the anthelmintic effect has still not been scientifically proven. Therefore, the present study aimed to evaluate the anthelmintic activity of L. zeylanica. The study began with the extraction of L. zeylanica extracts using methanol, ethanol, and aqueous. Subsequently, experiments were conducted to evaluate the possible in vitro anthelmintic activity of various extracts of L. zeylanica against earthworms. Various concentrations (25, 50 & 100 mg/mL) of extracts were tested and results were expressed in terms of the time of paralysis and time of death of worms. All extracts of the plant exhibited considerable anthelmintic activities in a dose-dependent manner. Of them all, methanolic extract at 100 mg/mL showed the most efficacious anthelmintic activity that was comparable to the reference drug (albendazole, 25 mg/mL). Therefore, we conclude that methanolic extract at the concentration of 100 mg/mL could be considered a candidate for worm treatment apart from the standard medication. This work may provide a framework for further study of L. zeylanica as an alternative treatment for worm infection. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22521/1/ME%207.pdf Hemagirri Manisekaran, and Muhammad Luqman Selahuddeen, and Zaidah Rahmat, and Faizuan Abdullah, and Abdul Fatah A. Samad, (2023) Evaluation of the in-vitro anthelmintic activity of Leucas zeylanica extracts on earthworms. Malaysian Applied Biology, 52 (4). pp. 57-64. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index
spellingShingle Hemagirri Manisekaran,
Muhammad Luqman Selahuddeen,
Zaidah Rahmat,
Faizuan Abdullah,
Abdul Fatah A. Samad,
Evaluation of the in-vitro anthelmintic activity of Leucas zeylanica extracts on earthworms
title Evaluation of the in-vitro anthelmintic activity of Leucas zeylanica extracts on earthworms
title_full Evaluation of the in-vitro anthelmintic activity of Leucas zeylanica extracts on earthworms
title_fullStr Evaluation of the in-vitro anthelmintic activity of Leucas zeylanica extracts on earthworms
title_full_unstemmed Evaluation of the in-vitro anthelmintic activity of Leucas zeylanica extracts on earthworms
title_short Evaluation of the in-vitro anthelmintic activity of Leucas zeylanica extracts on earthworms
title_sort evaluation of the in-vitro anthelmintic activity of leucas zeylanica extracts on earthworms
url http://journalarticle.ukm.my/22521/
http://journalarticle.ukm.my/22521/
http://journalarticle.ukm.my/22521/1/ME%207.pdf