Anti-microbial mechanism of Lonicera japonica and Andrographis paniculata extract in inhibiting Pseudomonas sp.
Antibiotic resistance reported in Pseudomonas sp. is associated with various opportunistic infections and is a concern in the public health system. The present study investigated the inhibitory effects of ethanol extracts of Lonicera japonica and Andrographis paniculata on Pseudomonas sp. The total...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2023
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| Online Access: | http://journalarticle.ukm.my/22506/ http://journalarticle.ukm.my/22506/1/MAS%204.pdf |
| _version_ | 1848815615726321664 |
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| author | Wong, Kok Kee Wong, Rui Rui Yuka Hara, |
| author_facet | Wong, Kok Kee Wong, Rui Rui Yuka Hara, |
| author_sort | Wong, Kok Kee |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Antibiotic resistance reported in Pseudomonas sp. is associated with various opportunistic infections and is a concern in the public health system. The present study investigated the inhibitory effects of ethanol extracts of Lonicera japonica and Andrographis paniculata on Pseudomonas sp. The total phenolic compound (TPC) calculated in gallic acid equivalent (GAE) was 508.6±117.96 mg GAE/100 g dried weight (DW) in L. japonica while A. paniculata contained 129.0 ± 21.00 mg GAE/100 g DW. Both herbs significantly reduced the swarming zone diameters of Pseudomonas sp., even at a concentration as low as 5 mg/mL when compared to the controls. The anti-swarming effect of L. japonica and A. paniculata may be one of the mutual mechanisms underlying their anti-microbial properties. However, only the extract of L. japonica showed cell anti-adhesion activity, which can prevent biofilm formation. Andrographis paniculata extract on the other hand shows the highest activity in disrupting the cell membrane of Pseudomonas sp. |
| first_indexed | 2025-11-15T00:52:48Z |
| format | Article |
| id | oai:generic.eprints.org:22506 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:52:48Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:225062023-11-20T07:52:23Z http://journalarticle.ukm.my/22506/ Anti-microbial mechanism of Lonicera japonica and Andrographis paniculata extract in inhibiting Pseudomonas sp. Wong, Kok Kee Wong, Rui Rui Yuka Hara, Antibiotic resistance reported in Pseudomonas sp. is associated with various opportunistic infections and is a concern in the public health system. The present study investigated the inhibitory effects of ethanol extracts of Lonicera japonica and Andrographis paniculata on Pseudomonas sp. The total phenolic compound (TPC) calculated in gallic acid equivalent (GAE) was 508.6±117.96 mg GAE/100 g dried weight (DW) in L. japonica while A. paniculata contained 129.0 ± 21.00 mg GAE/100 g DW. Both herbs significantly reduced the swarming zone diameters of Pseudomonas sp., even at a concentration as low as 5 mg/mL when compared to the controls. The anti-swarming effect of L. japonica and A. paniculata may be one of the mutual mechanisms underlying their anti-microbial properties. However, only the extract of L. japonica showed cell anti-adhesion activity, which can prevent biofilm formation. Andrographis paniculata extract on the other hand shows the highest activity in disrupting the cell membrane of Pseudomonas sp. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22506/1/MAS%204.pdf Wong, Kok Kee and Wong, Rui Rui and Yuka Hara, (2023) Anti-microbial mechanism of Lonicera japonica and Andrographis paniculata extract in inhibiting Pseudomonas sp. Malaysian Applied Biology, 52 (3). pp. 29-35. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
| spellingShingle | Wong, Kok Kee Wong, Rui Rui Yuka Hara, Anti-microbial mechanism of Lonicera japonica and Andrographis paniculata extract in inhibiting Pseudomonas sp. |
| title | Anti-microbial mechanism of Lonicera japonica and Andrographis paniculata extract in inhibiting Pseudomonas sp. |
| title_full | Anti-microbial mechanism of Lonicera japonica and Andrographis paniculata extract in inhibiting Pseudomonas sp. |
| title_fullStr | Anti-microbial mechanism of Lonicera japonica and Andrographis paniculata extract in inhibiting Pseudomonas sp. |
| title_full_unstemmed | Anti-microbial mechanism of Lonicera japonica and Andrographis paniculata extract in inhibiting Pseudomonas sp. |
| title_short | Anti-microbial mechanism of Lonicera japonica and Andrographis paniculata extract in inhibiting Pseudomonas sp. |
| title_sort | anti-microbial mechanism of lonicera japonica and andrographis paniculata extract in inhibiting pseudomonas sp. |
| url | http://journalarticle.ukm.my/22506/ http://journalarticle.ukm.my/22506/ http://journalarticle.ukm.my/22506/1/MAS%204.pdf |