In vitro biological activity of three marine sponges from theonella and haliclona genera collected from Bidong Island, Terengganu, Malaysia
Marine sponges are primitive sessile animals that are rich sources of biologically active compounds. This paper aimed to assess the in-vitro biological activity of marine sponges from Theonella and Haliclona genera collected from Bidong Island, Terengganu, Malaysia. Biological activities such as ant...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2023
|
| Online Access: | http://journalarticle.ukm.my/22404/ http://journalarticle.ukm.my/22404/1/MAD%206.pdf |
| _version_ | 1848815590891847680 |
|---|---|
| author | Nur Amira Jamaludin, Kamariah Bakar, Jasnizat Saidin, |
| author_facet | Nur Amira Jamaludin, Kamariah Bakar, Jasnizat Saidin, |
| author_sort | Nur Amira Jamaludin, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Marine sponges are primitive sessile animals that are rich sources of biologically active compounds. This paper aimed to assess the in-vitro biological activity of marine sponges from Theonella and Haliclona genera collected from Bidong Island, Terengganu, Malaysia. Biological activities such as antibacterial (discs diffusion assay), antioxidant (DPPH free-radical scavenging assay), and cytotoxicity activity (against cancerous HeLa, MCF-7, HepG-2 cell lines and the normal cell line Vero) were evaluated using MTT cytotoxicity assay. The bioassays were done on methanol extracts at different concentrations. Results indicate that T. swinhoei, and T. cf cupola showed low antibacterial capabilities ranging from 0 to 50 mg/mL and exhibited medium antioxidant activity with the IC50 value of 23.25 ± 1.57 and 18.52 ± 0.86 mg/mL, respectively. Cytotoxicity activities indicate that both species of T. swinhoei and T. cf cupola possesses toxic capabilities to inhibit the proliferation of all cancer cell lines used and demonstrated no significant toxicity for the normal cell line used in this study. Haliclona fascigera showed medium antibacterial activity against all Gram-positive bacteria and low activity against Gram-negative bacteria used. Haliclona fascigera exhibited antioxidant activity with an IC50 value of 1.80 ± 0.08 mg/mL and outcomes of the cytotoxicity activity assay against all cancer cells showed IC50 below 30 µg/mL. Marine sponges evaluated in this study indicate promising bioactive compounds that can be an excellent candidate for drug discovery in prospecting novel antibiotics and anticancer. Despite showing low antibacterial and medium antioxidant activity, species from both Theonella can be further studied in other assays to explore other biological activities whilst marine sponge H. fascigera possesses excellent capabilities in antibacterial, antioxidant, and cytotoxicity activities that can be further studied its chemical compositions for future research. |
| first_indexed | 2025-11-15T00:52:24Z |
| format | Article |
| id | oai:generic.eprints.org:22404 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:52:24Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:224042023-10-24T07:09:44Z http://journalarticle.ukm.my/22404/ In vitro biological activity of three marine sponges from theonella and haliclona genera collected from Bidong Island, Terengganu, Malaysia Nur Amira Jamaludin, Kamariah Bakar, Jasnizat Saidin, Marine sponges are primitive sessile animals that are rich sources of biologically active compounds. This paper aimed to assess the in-vitro biological activity of marine sponges from Theonella and Haliclona genera collected from Bidong Island, Terengganu, Malaysia. Biological activities such as antibacterial (discs diffusion assay), antioxidant (DPPH free-radical scavenging assay), and cytotoxicity activity (against cancerous HeLa, MCF-7, HepG-2 cell lines and the normal cell line Vero) were evaluated using MTT cytotoxicity assay. The bioassays were done on methanol extracts at different concentrations. Results indicate that T. swinhoei, and T. cf cupola showed low antibacterial capabilities ranging from 0 to 50 mg/mL and exhibited medium antioxidant activity with the IC50 value of 23.25 ± 1.57 and 18.52 ± 0.86 mg/mL, respectively. Cytotoxicity activities indicate that both species of T. swinhoei and T. cf cupola possesses toxic capabilities to inhibit the proliferation of all cancer cell lines used and demonstrated no significant toxicity for the normal cell line used in this study. Haliclona fascigera showed medium antibacterial activity against all Gram-positive bacteria and low activity against Gram-negative bacteria used. Haliclona fascigera exhibited antioxidant activity with an IC50 value of 1.80 ± 0.08 mg/mL and outcomes of the cytotoxicity activity assay against all cancer cells showed IC50 below 30 µg/mL. Marine sponges evaluated in this study indicate promising bioactive compounds that can be an excellent candidate for drug discovery in prospecting novel antibiotics and anticancer. Despite showing low antibacterial and medium antioxidant activity, species from both Theonella can be further studied in other assays to explore other biological activities whilst marine sponge H. fascigera possesses excellent capabilities in antibacterial, antioxidant, and cytotoxicity activities that can be further studied its chemical compositions for future research. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22404/1/MAD%206.pdf Nur Amira Jamaludin, and Kamariah Bakar, and Jasnizat Saidin, (2023) In vitro biological activity of three marine sponges from theonella and haliclona genera collected from Bidong Island, Terengganu, Malaysia. Malaysian Applied Biology, 52 (2). pp. 51-59. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
| spellingShingle | Nur Amira Jamaludin, Kamariah Bakar, Jasnizat Saidin, In vitro biological activity of three marine sponges from theonella and haliclona genera collected from Bidong Island, Terengganu, Malaysia |
| title | In vitro biological activity of three marine sponges from theonella and haliclona genera collected from Bidong Island, Terengganu, Malaysia |
| title_full | In vitro biological activity of three marine sponges from theonella and haliclona genera collected from Bidong Island, Terengganu, Malaysia |
| title_fullStr | In vitro biological activity of three marine sponges from theonella and haliclona genera collected from Bidong Island, Terengganu, Malaysia |
| title_full_unstemmed | In vitro biological activity of three marine sponges from theonella and haliclona genera collected from Bidong Island, Terengganu, Malaysia |
| title_short | In vitro biological activity of three marine sponges from theonella and haliclona genera collected from Bidong Island, Terengganu, Malaysia |
| title_sort | in vitro biological activity of three marine sponges from theonella and haliclona genera collected from bidong island, terengganu, malaysia |
| url | http://journalarticle.ukm.my/22404/ http://journalarticle.ukm.my/22404/ http://journalarticle.ukm.my/22404/1/MAD%206.pdf |