Decellularized and genipin crosslinked human umbilical cord artery and vein for potential use as peripheral nerve conduit
Critical gap peripheral nerve injury, commonly caused by motor vehicle accidents, results in dysfunctional nerve and impaired body function. Our study aims to develop a conduit from decellularized and genipin crosslinked human umbilical cord artery and vein for future use in critical nerve gap injur...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Kebangsaan Malaysia
2023
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| Online Access: | http://journalarticle.ukm.my/21930/ http://journalarticle.ukm.my/21930/1/ST%2020.pdf |
| _version_ | 1848815474044829696 |
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| author | Nabila Syahida Zailan, Nisriena Azlin Md Isa, Muhammad Asyraf Humayoon Kabir, Syahida Rabia Syed Ali, Muhamad Firdaus Norisman, Siti A. M. Imran, Mohamad Fikeri Ishak, Mohd Reusmaazran Yusof, Yogeswaran Lokanathan, |
| author_facet | Nabila Syahida Zailan, Nisriena Azlin Md Isa, Muhammad Asyraf Humayoon Kabir, Syahida Rabia Syed Ali, Muhamad Firdaus Norisman, Siti A. M. Imran, Mohamad Fikeri Ishak, Mohd Reusmaazran Yusof, Yogeswaran Lokanathan, |
| author_sort | Nabila Syahida Zailan, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Critical gap peripheral nerve injury, commonly caused by motor vehicle accidents, results in dysfunctional nerve and impaired body function. Our study aims to develop a conduit from decellularized and genipin crosslinked human umbilical cord artery and vein for future use in critical nerve gap injury treatments. Human umbilical cord arteries (HUCA) and veins (HUCV) were divided into native (nHUCA and nHUCV), decellularized (dHUCA and dHUCV) and genipin-crosslinked (clHUCA and clHUCV) groups. Both the decellularized and crosslinked groups were decellularized, and subsequently, the clHUCA and clHUCV groups were crosslinked with 0.1%, 0.4% and 0.7% (w/v) genipin. The HUCA and HUCV were then studied for decellularization efficiency, crosslinking index, biodegradation, swelling ratio, ultrastructure analysis, flexibility and mechanical strength. In addition, mesenchymal stem cells isolated from Wharton’s jelly were seeded into HUCA and HUCV for biocompatibility studies. The degradation test showed that nHUCV and dHUCV degraded at day 7 compared to other groups that did not show any degradation even after 21 days. Biocompatibility studies showed that the conduits crosslinked with 0.4% (w/v) genipin were successfully seeded and was having the most amount of seeded cells. In conclusion, the decellularization and genipin crosslinking of human umbilical cord artery and vein enabled successful in fabrication of conduit with suitable properties such as reduced swelling, flexibility, porosity and mechanical strength, with potential in tissue engineering applications. |
| first_indexed | 2025-11-15T00:50:33Z |
| format | Article |
| id | oai:generic.eprints.org:21930 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:50:33Z |
| publishDate | 2023 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:219302023-07-26T02:54:05Z http://journalarticle.ukm.my/21930/ Decellularized and genipin crosslinked human umbilical cord artery and vein for potential use as peripheral nerve conduit Nabila Syahida Zailan, Nisriena Azlin Md Isa, Muhammad Asyraf Humayoon Kabir, Syahida Rabia Syed Ali, Muhamad Firdaus Norisman, Siti A. M. Imran, Mohamad Fikeri Ishak, Mohd Reusmaazran Yusof, Yogeswaran Lokanathan, Critical gap peripheral nerve injury, commonly caused by motor vehicle accidents, results in dysfunctional nerve and impaired body function. Our study aims to develop a conduit from decellularized and genipin crosslinked human umbilical cord artery and vein for future use in critical nerve gap injury treatments. Human umbilical cord arteries (HUCA) and veins (HUCV) were divided into native (nHUCA and nHUCV), decellularized (dHUCA and dHUCV) and genipin-crosslinked (clHUCA and clHUCV) groups. Both the decellularized and crosslinked groups were decellularized, and subsequently, the clHUCA and clHUCV groups were crosslinked with 0.1%, 0.4% and 0.7% (w/v) genipin. The HUCA and HUCV were then studied for decellularization efficiency, crosslinking index, biodegradation, swelling ratio, ultrastructure analysis, flexibility and mechanical strength. In addition, mesenchymal stem cells isolated from Wharton’s jelly were seeded into HUCA and HUCV for biocompatibility studies. The degradation test showed that nHUCV and dHUCV degraded at day 7 compared to other groups that did not show any degradation even after 21 days. Biocompatibility studies showed that the conduits crosslinked with 0.4% (w/v) genipin were successfully seeded and was having the most amount of seeded cells. In conclusion, the decellularization and genipin crosslinking of human umbilical cord artery and vein enabled successful in fabrication of conduit with suitable properties such as reduced swelling, flexibility, porosity and mechanical strength, with potential in tissue engineering applications. Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21930/1/ST%2020.pdf Nabila Syahida Zailan, and Nisriena Azlin Md Isa, and Muhammad Asyraf Humayoon Kabir, and Syahida Rabia Syed Ali, and Muhamad Firdaus Norisman, and Siti A. M. Imran, and Mohamad Fikeri Ishak, and Mohd Reusmaazran Yusof, and Yogeswaran Lokanathan, (2023) Decellularized and genipin crosslinked human umbilical cord artery and vein for potential use as peripheral nerve conduit. Sains Malaysiana, 52 (3). pp. 967-979. ISSN 0126-6039 http://www.ukm.my/jsm/index.html |
| spellingShingle | Nabila Syahida Zailan, Nisriena Azlin Md Isa, Muhammad Asyraf Humayoon Kabir, Syahida Rabia Syed Ali, Muhamad Firdaus Norisman, Siti A. M. Imran, Mohamad Fikeri Ishak, Mohd Reusmaazran Yusof, Yogeswaran Lokanathan, Decellularized and genipin crosslinked human umbilical cord artery and vein for potential use as peripheral nerve conduit |
| title | Decellularized and genipin crosslinked human umbilical cord artery and vein for potential use as peripheral nerve conduit |
| title_full | Decellularized and genipin crosslinked human umbilical cord artery and vein for potential use as peripheral nerve conduit |
| title_fullStr | Decellularized and genipin crosslinked human umbilical cord artery and vein for potential use as peripheral nerve conduit |
| title_full_unstemmed | Decellularized and genipin crosslinked human umbilical cord artery and vein for potential use as peripheral nerve conduit |
| title_short | Decellularized and genipin crosslinked human umbilical cord artery and vein for potential use as peripheral nerve conduit |
| title_sort | decellularized and genipin crosslinked human umbilical cord artery and vein for potential use as peripheral nerve conduit |
| url | http://journalarticle.ukm.my/21930/ http://journalarticle.ukm.my/21930/ http://journalarticle.ukm.my/21930/1/ST%2020.pdf |