Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell
It is known that the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) has the potential for chemoprevention in MCF-7 cells. However, the expression of Bcl-2 and Bax genes was associated with cancer. Therefore, this study aimed to analyze the Bcl-2 and Bax gene expression induced by t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/21878/ http://journalarticle.ukm.my/21878/1/MT%207.pdf |
| _version_ | 1848815460691214336 |
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| author | Hanif, Monica Mulnia Tjong, Djong Hon Syaifullah, Santoso, Putra Efrizal, Roesma, Dewi Imelda |
| author_facet | Hanif, Monica Mulnia Tjong, Djong Hon Syaifullah, Santoso, Putra Efrizal, Roesma, Dewi Imelda |
| author_sort | Hanif, Monica Mulnia |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | It is known that the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) has the potential for chemoprevention in MCF-7 cells. However, the expression of Bcl-2 and Bax genes was associated with cancer. Therefore, this study aimed to analyze the Bcl-2 and Bax gene expression induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in MCF-7. The method in this study was an in vitro experiment using MCF-7 control cells and MCF-7 cells induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus), and Real-time PCR was used to examine the expression of Bcl-2 and Bax genes. The result showed that the expression of the Bcl-2 gene had an insignificant decrease (p>0.05) by 15%, and the expression of the Bax gene had a significant increase (p<0.05) by 489% compared to the control. These results support that ovarian extract Singkarak Lake Pufferfish (Tetraodon leiurus) can be alternative cancer chemoprevention. |
| first_indexed | 2025-11-15T00:50:20Z |
| format | Article |
| id | oai:generic.eprints.org:21878 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:50:20Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:218782023-07-11T08:17:01Z http://journalarticle.ukm.my/21878/ Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell Hanif, Monica Mulnia Tjong, Djong Hon Syaifullah, Santoso, Putra Efrizal, Roesma, Dewi Imelda It is known that the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) has the potential for chemoprevention in MCF-7 cells. However, the expression of Bcl-2 and Bax genes was associated with cancer. Therefore, this study aimed to analyze the Bcl-2 and Bax gene expression induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in MCF-7. The method in this study was an in vitro experiment using MCF-7 control cells and MCF-7 cells induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus), and Real-time PCR was used to examine the expression of Bcl-2 and Bax genes. The result showed that the expression of the Bcl-2 gene had an insignificant decrease (p>0.05) by 15%, and the expression of the Bax gene had a significant increase (p<0.05) by 489% compared to the control. These results support that ovarian extract Singkarak Lake Pufferfish (Tetraodon leiurus) can be alternative cancer chemoprevention. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21878/1/MT%207.pdf Hanif, Monica Mulnia and Tjong, Djong Hon and Syaifullah, and Santoso, Putra and Efrizal, and Roesma, Dewi Imelda (2022) Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell. Malaysian Applied Biology, 51 (3). pp. 63-69. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
| spellingShingle | Hanif, Monica Mulnia Tjong, Djong Hon Syaifullah, Santoso, Putra Efrizal, Roesma, Dewi Imelda Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell |
| title | Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell |
| title_full | Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell |
| title_fullStr | Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell |
| title_full_unstemmed | Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell |
| title_short | Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell |
| title_sort | expression of bcl-2 and bax genes induced by an ovarian extract of singkarak lake pufferfish (tetraodon leiurus) in breast cancer cell |
| url | http://journalarticle.ukm.my/21878/ http://journalarticle.ukm.my/21878/ http://journalarticle.ukm.my/21878/1/MT%207.pdf |