Optimizing in vitro surface sterilization of Cyathea latebrosa spore
Cyathea latebrosa is one of the lowland tree fern species found in Peninsular Malaysia. This fern species is highly demanded in ornamental landscaping. The in vitro cultures are an important tool for propagation which may contribute toward the reduction of over-exploitation. To overcome these proble...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/21705/ http://journalarticle.ukm.my/21705/1/ML%2015.pdf |
| _version_ | 1848815417139658752 |
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| author | Nurul Nadhirah, Haja Maideen, Zuraida Ab Rahman, Ayu Nazreena Othman, |
| author_facet | Nurul Nadhirah, Haja Maideen, Zuraida Ab Rahman, Ayu Nazreena Othman, |
| author_sort | Nurul Nadhirah, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Cyathea latebrosa is one of the lowland tree fern species found in Peninsular Malaysia. This fern species is highly demanded in ornamental landscaping. The in vitro cultures are an important tool for propagation which may contribute toward the reduction of over-exploitation. To overcome these problems, an effective spore surface disinfection protocol is crucial to allow the germination stage to be carried out. This studied had carried out three types of methods which are the packet method (PM), centrifuged method (CM), and soak method (SM) with difference percent of concentration (0, 0.1, 0.5, 1.0, 10 & 30) Mercury Chloride (HgCl2) and Sodium hypochlorite (NaOCl). In contrast, the method and concentration of disinfection affect germination. Our results showed that the soak method in both types of disinfection is a significant difference due to statistical analysis (MANOVA) which gives a positive effect on the germination of the spore. This method is efficient for sterilizing which spore loss is kept to a minimum and has a higher rate of germination (HgCl2-90% & NaOCl-80%). The optimum concentration of HgCl2 was 0.1%, then followed by 0.5% and 1.0%, while for NaOCl was 30%, 20%, and 10%. |
| first_indexed | 2025-11-15T00:49:39Z |
| format | Article |
| id | oai:generic.eprints.org:21705 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:49:39Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:217052023-06-08T08:04:34Z http://journalarticle.ukm.my/21705/ Optimizing in vitro surface sterilization of Cyathea latebrosa spore Nurul Nadhirah, Haja Maideen, Zuraida Ab Rahman, Ayu Nazreena Othman, Cyathea latebrosa is one of the lowland tree fern species found in Peninsular Malaysia. This fern species is highly demanded in ornamental landscaping. The in vitro cultures are an important tool for propagation which may contribute toward the reduction of over-exploitation. To overcome these problems, an effective spore surface disinfection protocol is crucial to allow the germination stage to be carried out. This studied had carried out three types of methods which are the packet method (PM), centrifuged method (CM), and soak method (SM) with difference percent of concentration (0, 0.1, 0.5, 1.0, 10 & 30) Mercury Chloride (HgCl2) and Sodium hypochlorite (NaOCl). In contrast, the method and concentration of disinfection affect germination. Our results showed that the soak method in both types of disinfection is a significant difference due to statistical analysis (MANOVA) which gives a positive effect on the germination of the spore. This method is efficient for sterilizing which spore loss is kept to a minimum and has a higher rate of germination (HgCl2-90% & NaOCl-80%). The optimum concentration of HgCl2 was 0.1%, then followed by 0.5% and 1.0%, while for NaOCl was 30%, 20%, and 10%. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21705/1/ML%2015.pdf Nurul Nadhirah, and Haja Maideen, and Zuraida Ab Rahman, and Ayu Nazreena Othman, (2022) Optimizing in vitro surface sterilization of Cyathea latebrosa spore. Malaysian Applied Biology, 51 (5). pp. 159-163. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
| spellingShingle | Nurul Nadhirah, Haja Maideen, Zuraida Ab Rahman, Ayu Nazreena Othman, Optimizing in vitro surface sterilization of Cyathea latebrosa spore |
| title | Optimizing in vitro surface sterilization of Cyathea latebrosa spore |
| title_full | Optimizing in vitro surface sterilization of Cyathea latebrosa spore |
| title_fullStr | Optimizing in vitro surface sterilization of Cyathea latebrosa spore |
| title_full_unstemmed | Optimizing in vitro surface sterilization of Cyathea latebrosa spore |
| title_short | Optimizing in vitro surface sterilization of Cyathea latebrosa spore |
| title_sort | optimizing in vitro surface sterilization of cyathea latebrosa spore |
| url | http://journalarticle.ukm.my/21705/ http://journalarticle.ukm.my/21705/ http://journalarticle.ukm.my/21705/1/ML%2015.pdf |