Chlorella sp. (UKM8), a local microalgae isolate with anti-human herpes virus and antioxidant properties

Microalgae are an invaluable source of new and safe therapeutics with potential antiviral and free-radical scavenging compounds. This study aimed to investigate the antiviral and antioxidant properties of local microalgae, Chlorella sp. (UKM8). The UKM8 methanol extract (UKM8-ME) was tested for anti...

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Main Authors: Abdul Fattah Shaima, Nazlina Haiza Mohd Yasin, Nazlina Ibrahim
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2022
Online Access:http://journalarticle.ukm.my/21704/
http://journalarticle.ukm.my/21704/1/ML%2014.pdf
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author Abdul Fattah Shaima,
Nazlina Haiza Mohd Yasin,
Nazlina Ibrahim,
author_facet Abdul Fattah Shaima,
Nazlina Haiza Mohd Yasin,
Nazlina Ibrahim,
author_sort Abdul Fattah Shaima,
building UKM Institutional Repository
collection Online Access
description Microalgae are an invaluable source of new and safe therapeutics with potential antiviral and free-radical scavenging compounds. This study aimed to investigate the antiviral and antioxidant properties of local microalgae, Chlorella sp. (UKM8). The UKM8 methanol extract (UKM8-ME) was tested for antiviral activity using plaque reduction assay against Human Herpes Virus type 1 (HHV-1). The antioxidant activity of UKM8-ME was evaluated for the radical scavenging activity (RSA) according to the elimination of 1,1-diphenyl-2-pikrilhydrazil (DPPH) radicals and total phenolic content (TPC) by the Folin-Ciocalteu reactions. UKM8-ME effective concentration that inhibits 50% (EC50) of plaque formation was 222.33 ± 24.54 μg/mL. The calculated selective index is 19 indicating potential antiviral activity. In the DPPH assay, the IC50 value of positive control and UKM8-ME were 122.9 ± 29.1 and 198.78 ± 14.35 μg/mL, respectively. The TPC of positive control and UKM8-ME were 263.414 ± 9.6 and 254.793 ± 3.31 mg GAE/g, respectively. Evaluation in RSA and TPC concludes that UKM8-ME has high antioxidant activity. In conclusion, UKM8-ME has two unique properties in anti-HHV-1 and antioxidant activities that can be further evaluated for potential in pharmaceutics and food ingredients.
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spelling oai:generic.eprints.org:217042023-06-08T07:52:55Z http://journalarticle.ukm.my/21704/ Chlorella sp. (UKM8), a local microalgae isolate with anti-human herpes virus and antioxidant properties Abdul Fattah Shaima, Nazlina Haiza Mohd Yasin, Nazlina Ibrahim, Microalgae are an invaluable source of new and safe therapeutics with potential antiviral and free-radical scavenging compounds. This study aimed to investigate the antiviral and antioxidant properties of local microalgae, Chlorella sp. (UKM8). The UKM8 methanol extract (UKM8-ME) was tested for antiviral activity using plaque reduction assay against Human Herpes Virus type 1 (HHV-1). The antioxidant activity of UKM8-ME was evaluated for the radical scavenging activity (RSA) according to the elimination of 1,1-diphenyl-2-pikrilhydrazil (DPPH) radicals and total phenolic content (TPC) by the Folin-Ciocalteu reactions. UKM8-ME effective concentration that inhibits 50% (EC50) of plaque formation was 222.33 ± 24.54 μg/mL. The calculated selective index is 19 indicating potential antiviral activity. In the DPPH assay, the IC50 value of positive control and UKM8-ME were 122.9 ± 29.1 and 198.78 ± 14.35 μg/mL, respectively. The TPC of positive control and UKM8-ME were 263.414 ± 9.6 and 254.793 ± 3.31 mg GAE/g, respectively. Evaluation in RSA and TPC concludes that UKM8-ME has high antioxidant activity. In conclusion, UKM8-ME has two unique properties in anti-HHV-1 and antioxidant activities that can be further evaluated for potential in pharmaceutics and food ingredients. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21704/1/ML%2014.pdf Abdul Fattah Shaima, and Nazlina Haiza Mohd Yasin, and Nazlina Ibrahim, (2022) Chlorella sp. (UKM8), a local microalgae isolate with anti-human herpes virus and antioxidant properties. Malaysian Applied Biology, 51 (5). pp. 153-158. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index
spellingShingle Abdul Fattah Shaima,
Nazlina Haiza Mohd Yasin,
Nazlina Ibrahim,
Chlorella sp. (UKM8), a local microalgae isolate with anti-human herpes virus and antioxidant properties
title Chlorella sp. (UKM8), a local microalgae isolate with anti-human herpes virus and antioxidant properties
title_full Chlorella sp. (UKM8), a local microalgae isolate with anti-human herpes virus and antioxidant properties
title_fullStr Chlorella sp. (UKM8), a local microalgae isolate with anti-human herpes virus and antioxidant properties
title_full_unstemmed Chlorella sp. (UKM8), a local microalgae isolate with anti-human herpes virus and antioxidant properties
title_short Chlorella sp. (UKM8), a local microalgae isolate with anti-human herpes virus and antioxidant properties
title_sort chlorella sp. (ukm8), a local microalgae isolate with anti-human herpes virus and antioxidant properties
url http://journalarticle.ukm.my/21704/
http://journalarticle.ukm.my/21704/
http://journalarticle.ukm.my/21704/1/ML%2014.pdf