Effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group
Skin aging causes delayed re-epithelialisation and impaired wound healing. Thus, supplementation of wound healing mediators and extracellular matrix (ECM) components may be a potential treatment strategy for age-related impaired wound healing. Fibroblasts secrete wound-healing factors and can be col...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2023
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| Online Access: | http://journalarticle.ukm.my/21547/ http://journalarticle.ukm.my/21547/1/S%2018.pdf |
| _version_ | 1848815378664259584 |
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| author | Nurul ‘Izzah Abdul Ghani, Manira Maarof, Shiplu Roy Chowdhury, Aminuddin Saim, Ruszymah Idrus, Fauzi Mh Busra, |
| author_facet | Nurul ‘Izzah Abdul Ghani, Manira Maarof, Shiplu Roy Chowdhury, Aminuddin Saim, Ruszymah Idrus, Fauzi Mh Busra, |
| author_sort | Nurul ‘Izzah Abdul Ghani, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Skin aging causes delayed re-epithelialisation and impaired wound healing. Thus, supplementation of wound healing mediators and extracellular matrix (ECM) components may be a potential treatment strategy for age-related impaired wound healing. Fibroblasts secrete wound-healing factors and can be collected from used medium, i.e., dermal fibroblast conditioned medium (DFCM). In this study, we elucidated the effect of DFCM on the in vitro wound healing of keratinocytes isolated from different age groups (≥18–35, 36–54, ≥55 years) via cell attachment, growth rate, and wound healing rate assays. The DFCM was prepared by culturing confluent fibroblasts with serum-free keratinocytespecific (DFCM-KM) and fibroblast-specific (DFCM-FM) medium. The cell attachment efficiency decreased with the increase of age. However, keratinocyte attachment was enhanced in the DFCM-KM group, where it was 1.24, 1.27, and 1.32 times higher of cells concentration for the ≥18–35-, 36–54-, and ≥55-year age groups, respectively, as compared to the control group. The keratinocytes from each age group demonstrated a similar growth profile for all culture conditions, where the DFCM-KM group exhibited a comparable growth rate with the control group whilst the DFCM-FM group exhibited a significantly lower growth rate compared to the other groups. In contrast, the DFCM-FM group demonstrated a significantly higher healing rate in all age groups as compared to the DFCM-KM and control groups. However, there was no significant difference between the healing rates of the DFCM-KM and control groups. In conclusion, DFCM-KM enhanced keratinocyte attachment while DFCM-FM enhanced the keratinocyte healing rate irrespective of donor age, which indicated the potential application of DFCM in wound healing in aged skin. |
| first_indexed | 2025-11-15T00:49:02Z |
| format | Article |
| id | oai:generic.eprints.org:21547 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:49:02Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:215472023-05-09T04:06:40Z http://journalarticle.ukm.my/21547/ Effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group Nurul ‘Izzah Abdul Ghani, Manira Maarof, Shiplu Roy Chowdhury, Aminuddin Saim, Ruszymah Idrus, Fauzi Mh Busra, Skin aging causes delayed re-epithelialisation and impaired wound healing. Thus, supplementation of wound healing mediators and extracellular matrix (ECM) components may be a potential treatment strategy for age-related impaired wound healing. Fibroblasts secrete wound-healing factors and can be collected from used medium, i.e., dermal fibroblast conditioned medium (DFCM). In this study, we elucidated the effect of DFCM on the in vitro wound healing of keratinocytes isolated from different age groups (≥18–35, 36–54, ≥55 years) via cell attachment, growth rate, and wound healing rate assays. The DFCM was prepared by culturing confluent fibroblasts with serum-free keratinocytespecific (DFCM-KM) and fibroblast-specific (DFCM-FM) medium. The cell attachment efficiency decreased with the increase of age. However, keratinocyte attachment was enhanced in the DFCM-KM group, where it was 1.24, 1.27, and 1.32 times higher of cells concentration for the ≥18–35-, 36–54-, and ≥55-year age groups, respectively, as compared to the control group. The keratinocytes from each age group demonstrated a similar growth profile for all culture conditions, where the DFCM-KM group exhibited a comparable growth rate with the control group whilst the DFCM-FM group exhibited a significantly lower growth rate compared to the other groups. In contrast, the DFCM-FM group demonstrated a significantly higher healing rate in all age groups as compared to the DFCM-KM and control groups. However, there was no significant difference between the healing rates of the DFCM-KM and control groups. In conclusion, DFCM-KM enhanced keratinocyte attachment while DFCM-FM enhanced the keratinocyte healing rate irrespective of donor age, which indicated the potential application of DFCM in wound healing in aged skin. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21547/1/S%2018.pdf Nurul ‘Izzah Abdul Ghani, and Manira Maarof, and Shiplu Roy Chowdhury, and Aminuddin Saim, and Ruszymah Idrus, and Fauzi Mh Busra, (2023) Effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group. Sains Malaysiana, 52 (1). pp. 223-231. ISSN 0126-6039 http://www.ukm.my/jsm/index.html |
| spellingShingle | Nurul ‘Izzah Abdul Ghani, Manira Maarof, Shiplu Roy Chowdhury, Aminuddin Saim, Ruszymah Idrus, Fauzi Mh Busra, Effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group |
| title | Effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group |
| title_full | Effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group |
| title_fullStr | Effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group |
| title_full_unstemmed | Effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group |
| title_short | Effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group |
| title_sort | effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group |
| url | http://journalarticle.ukm.my/21547/ http://journalarticle.ukm.my/21547/ http://journalarticle.ukm.my/21547/1/S%2018.pdf |