Effect of swamp Eel (Monopterus albus) plasma on the quality of tilapia (Oreochromis mossambicus) Surimi

The effects of crude plasma and ethanol-extracted of swamp eel plasma at different concentrations (0, 0.25, 0.5, 0.75, 1, 1.5, 2 mg/g) as inhibitor protease in the quality of tilapia (Oreochromis mossambicus) surimi were investigated. The parameters analyzed were gel strength, expressible moisture,...

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Main Authors: Nopianti, Rodiana, Chasanah, Ekowati, Sukarno, Suhartono, Maggy Thenawidjaja
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/21536/
http://journalarticle.ukm.my/21536/1/S%208.pdf
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author Nopianti, Rodiana
Chasanah, Ekowati
Sukarno,
Suhartono, Maggy Thenawidjaja
author_facet Nopianti, Rodiana
Chasanah, Ekowati
Sukarno,
Suhartono, Maggy Thenawidjaja
author_sort Nopianti, Rodiana
building UKM Institutional Repository
collection Online Access
description The effects of crude plasma and ethanol-extracted of swamp eel plasma at different concentrations (0, 0.25, 0.5, 0.75, 1, 1.5, 2 mg/g) as inhibitor protease in the quality of tilapia (Oreochromis mossambicus) surimi were investigated. The parameters analyzed were gel strength, expressible moisture, whiteness, microstructure, protein solubility, hydrolysis pattern of surimi by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, percent inhibition, and zymogram. The results showed that ethanol-extracted plasma could maintain gel strength, expressible moisture, microstructure, protein solubility, and percent inhibition in lysis of tilapia surimi from protease activity that caused formation of modori structure better than crude plasma. The whiteness decreased as the concentration of plasma used increased. SDS-PAGE for hydrolysis pattern analysis showed that the effectiveness of crude plasma as a protease inhibitor decreased as the concentration increased. There was an increase in the inhibition of protease in the ethanol-extracted plasma at a concentration of 1.5 mg/g. It suggested that the crude plasma contained an internal protease enzyme that might interfere with the reduced myosin intensity.
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spelling oai:generic.eprints.org:215362023-05-08T08:55:43Z http://journalarticle.ukm.my/21536/ Effect of swamp Eel (Monopterus albus) plasma on the quality of tilapia (Oreochromis mossambicus) Surimi Nopianti, Rodiana Chasanah, Ekowati Sukarno, Suhartono, Maggy Thenawidjaja The effects of crude plasma and ethanol-extracted of swamp eel plasma at different concentrations (0, 0.25, 0.5, 0.75, 1, 1.5, 2 mg/g) as inhibitor protease in the quality of tilapia (Oreochromis mossambicus) surimi were investigated. The parameters analyzed were gel strength, expressible moisture, whiteness, microstructure, protein solubility, hydrolysis pattern of surimi by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, percent inhibition, and zymogram. The results showed that ethanol-extracted plasma could maintain gel strength, expressible moisture, microstructure, protein solubility, and percent inhibition in lysis of tilapia surimi from protease activity that caused formation of modori structure better than crude plasma. The whiteness decreased as the concentration of plasma used increased. SDS-PAGE for hydrolysis pattern analysis showed that the effectiveness of crude plasma as a protease inhibitor decreased as the concentration increased. There was an increase in the inhibition of protease in the ethanol-extracted plasma at a concentration of 1.5 mg/g. It suggested that the crude plasma contained an internal protease enzyme that might interfere with the reduced myosin intensity. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21536/1/S%208.pdf Nopianti, Rodiana and Chasanah, Ekowati and Sukarno, and Suhartono, Maggy Thenawidjaja (2023) Effect of swamp Eel (Monopterus albus) plasma on the quality of tilapia (Oreochromis mossambicus) Surimi. Sains Malaysiana, 52 (1). pp. 95-105. ISSN 0126-6039 http://www.ukm.my/jsm/index.html
spellingShingle Nopianti, Rodiana
Chasanah, Ekowati
Sukarno,
Suhartono, Maggy Thenawidjaja
Effect of swamp Eel (Monopterus albus) plasma on the quality of tilapia (Oreochromis mossambicus) Surimi
title Effect of swamp Eel (Monopterus albus) plasma on the quality of tilapia (Oreochromis mossambicus) Surimi
title_full Effect of swamp Eel (Monopterus albus) plasma on the quality of tilapia (Oreochromis mossambicus) Surimi
title_fullStr Effect of swamp Eel (Monopterus albus) plasma on the quality of tilapia (Oreochromis mossambicus) Surimi
title_full_unstemmed Effect of swamp Eel (Monopterus albus) plasma on the quality of tilapia (Oreochromis mossambicus) Surimi
title_short Effect of swamp Eel (Monopterus albus) plasma on the quality of tilapia (Oreochromis mossambicus) Surimi
title_sort effect of swamp eel (monopterus albus) plasma on the quality of tilapia (oreochromis mossambicus) surimi
url http://journalarticle.ukm.my/21536/
http://journalarticle.ukm.my/21536/
http://journalarticle.ukm.my/21536/1/S%208.pdf