Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4
The study aimed at identifying and purifying cytosolic glutathione transferase isoforms expressed in Pseudomonas sp. UW4. Search at UniProt (https://www.uniprot.org/uniprot/), has indicated that there were 20 genes encoding putative glutathione transferases for the microorganism. The molecular weigh...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/21464/ http://journalarticle.ukm.my/21464/1/M%2025.pdf |
| _version_ | 1848815357338320896 |
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| author | Kong, Christina Wen Hui Tan, Irene Kit Ping Zazali Alias, |
| author_facet | Kong, Christina Wen Hui Tan, Irene Kit Ping Zazali Alias, |
| author_sort | Kong, Christina Wen Hui |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The study aimed at identifying and purifying cytosolic glutathione transferase isoforms expressed in Pseudomonas sp. UW4. Search at UniProt (https://www.uniprot.org/uniprot/), has indicated that there were 20 genes encoding putative glutathione transferases for the microorganism. The molecular weights of the isoforms ranged from 17.6 to 34.06 kDa. SDS-polyacrylamide gel electrophoresis revealed that the GST purified using Sulfobromophthalein-glutathione (BSP) affinity column, resolved into a single band with a low molecular weight (MW) of 16 kDa with the pI value of 6.0. Purified GST was reactive towards ethacrynic acid, 1-chloro-2,4-dinitrobenzene, cumene hydroxide, and hydrogen peroxide, but no detectable activity with Trans-2-octenal, hepta-2,4-dienal and Trans-4-phenyl-3-butene-2-one. This has proven that putative GST possessed peroxidase activity and proposed to be similar to PputUW4_00801 (putative glutathione S-transferase) of Pseudomonas sp. UW4 according to its estimated molecular weight and the pI values obtained experimentally. |
| first_indexed | 2025-11-15T00:48:42Z |
| format | Article |
| id | oai:generic.eprints.org:21464 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:48:42Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:214642023-04-07T00:54:46Z http://journalarticle.ukm.my/21464/ Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4 Kong, Christina Wen Hui Tan, Irene Kit Ping Zazali Alias, The study aimed at identifying and purifying cytosolic glutathione transferase isoforms expressed in Pseudomonas sp. UW4. Search at UniProt (https://www.uniprot.org/uniprot/), has indicated that there were 20 genes encoding putative glutathione transferases for the microorganism. The molecular weights of the isoforms ranged from 17.6 to 34.06 kDa. SDS-polyacrylamide gel electrophoresis revealed that the GST purified using Sulfobromophthalein-glutathione (BSP) affinity column, resolved into a single band with a low molecular weight (MW) of 16 kDa with the pI value of 6.0. Purified GST was reactive towards ethacrynic acid, 1-chloro-2,4-dinitrobenzene, cumene hydroxide, and hydrogen peroxide, but no detectable activity with Trans-2-octenal, hepta-2,4-dienal and Trans-4-phenyl-3-butene-2-one. This has proven that putative GST possessed peroxidase activity and proposed to be similar to PputUW4_00801 (putative glutathione S-transferase) of Pseudomonas sp. UW4 according to its estimated molecular weight and the pI values obtained experimentally. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21464/1/M%2025.pdf Kong, Christina Wen Hui and Tan, Irene Kit Ping and Zazali Alias, (2022) Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4. Malaysian Applied Biology, 51 (4). pp. 177-184. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
| spellingShingle | Kong, Christina Wen Hui Tan, Irene Kit Ping Zazali Alias, Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4 |
| title | Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4 |
| title_full | Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4 |
| title_fullStr | Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4 |
| title_full_unstemmed | Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4 |
| title_short | Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4 |
| title_sort | bioinformatic analysis and purification of glutathione transferase (gst) from pseudomonas sp. uw4 |
| url | http://journalarticle.ukm.my/21464/ http://journalarticle.ukm.my/21464/ http://journalarticle.ukm.my/21464/1/M%2025.pdf |