Effects of palm oil derived tocotrienol rich fraction and vitamin e isomers on biomarkers of early atherogenesis in stimulated human umbilical vein endothelial cells
This study was conducted to investigate the effects of tocotrienol rich fraction (TRF), α-TOC, and pure TCT isomers (α-. γ- & δ-TCT) on inflammation, endothelial activation, nuclear factor kappa B (NFκB), endothelial nitric oxide synthase (eNOS) and monocyte binding activity (MBA) in vitro. Huma...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/21432/ http://journalarticle.ukm.my/21432/1/M%2018.pdf |
| _version_ | 1848815351337320448 |
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| author | Suhaila Abd Muid, Froemming, Gabriele Anisah Ruth, Abd Manaf Ali, Thuhairah Hasrah Abdul Rahman, Zalina Hamid, Hapizah Nawawi, |
| author_facet | Suhaila Abd Muid, Froemming, Gabriele Anisah Ruth, Abd Manaf Ali, Thuhairah Hasrah Abdul Rahman, Zalina Hamid, Hapizah Nawawi, |
| author_sort | Suhaila Abd Muid, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | This study was conducted to investigate the effects of tocotrienol rich fraction (TRF), α-TOC, and pure TCT isomers (α-. γ- & δ-TCT) on inflammation, endothelial activation, nuclear factor kappa B (NFκB), endothelial nitric oxide synthase (eNOS) and monocyte binding activity (MBA) in vitro. Human umbilical vein endothelial cells (HUVECs) were incubated with various concentrations of α-TOC, pure TCT isomers and TRF (0.3-10 μM) together with lipopolysaccharides (LPS) for 16 h. Culture medium and cells were collected and measured for the protein and gene expression of IL-6, TNF-α, NFκB, ICAM-1, VCAM-1, e-selectin, and eNOS. Monocyte binding activity (MBA) was measured by Rose Bengal staining. Area under the curve (AUC) analysis revealed that TRF and pure TCT particularly γ- and δ- isomers, showed better inhibition of inflammation and endothelial activation, MBA and greater eNOS increment than α-TOC. These suggest that TRF and pure TCT isomers have potential as preventive anti-atherogenic agents by attenuating the release of early biomarkers of atherogenesis which is better than α-TOC in LPS-stimulated human endothelial cells. |
| first_indexed | 2025-11-15T00:48:36Z |
| format | Article |
| id | oai:generic.eprints.org:21432 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:48:36Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:214322023-04-06T07:23:27Z http://journalarticle.ukm.my/21432/ Effects of palm oil derived tocotrienol rich fraction and vitamin e isomers on biomarkers of early atherogenesis in stimulated human umbilical vein endothelial cells Suhaila Abd Muid, Froemming, Gabriele Anisah Ruth, Abd Manaf Ali, Thuhairah Hasrah Abdul Rahman, Zalina Hamid, Hapizah Nawawi, This study was conducted to investigate the effects of tocotrienol rich fraction (TRF), α-TOC, and pure TCT isomers (α-. γ- & δ-TCT) on inflammation, endothelial activation, nuclear factor kappa B (NFκB), endothelial nitric oxide synthase (eNOS) and monocyte binding activity (MBA) in vitro. Human umbilical vein endothelial cells (HUVECs) were incubated with various concentrations of α-TOC, pure TCT isomers and TRF (0.3-10 μM) together with lipopolysaccharides (LPS) for 16 h. Culture medium and cells were collected and measured for the protein and gene expression of IL-6, TNF-α, NFκB, ICAM-1, VCAM-1, e-selectin, and eNOS. Monocyte binding activity (MBA) was measured by Rose Bengal staining. Area under the curve (AUC) analysis revealed that TRF and pure TCT particularly γ- and δ- isomers, showed better inhibition of inflammation and endothelial activation, MBA and greater eNOS increment than α-TOC. These suggest that TRF and pure TCT isomers have potential as preventive anti-atherogenic agents by attenuating the release of early biomarkers of atherogenesis which is better than α-TOC in LPS-stimulated human endothelial cells. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21432/1/M%2018.pdf Suhaila Abd Muid, and Froemming, Gabriele Anisah Ruth, and Abd Manaf Ali, and Thuhairah Hasrah Abdul Rahman, and Zalina Hamid, and Hapizah Nawawi, (2022) Effects of palm oil derived tocotrienol rich fraction and vitamin e isomers on biomarkers of early atherogenesis in stimulated human umbilical vein endothelial cells. Malaysian Applied Biology, 51 (4). pp. 145-152. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
| spellingShingle | Suhaila Abd Muid, Froemming, Gabriele Anisah Ruth, Abd Manaf Ali, Thuhairah Hasrah Abdul Rahman, Zalina Hamid, Hapizah Nawawi, Effects of palm oil derived tocotrienol rich fraction and vitamin e isomers on biomarkers of early atherogenesis in stimulated human umbilical vein endothelial cells |
| title | Effects of palm oil derived tocotrienol rich fraction and vitamin e isomers on biomarkers of early atherogenesis in stimulated human umbilical vein endothelial cells |
| title_full | Effects of palm oil derived tocotrienol rich fraction and vitamin e isomers on biomarkers of early atherogenesis in stimulated human umbilical vein endothelial cells |
| title_fullStr | Effects of palm oil derived tocotrienol rich fraction and vitamin e isomers on biomarkers of early atherogenesis in stimulated human umbilical vein endothelial cells |
| title_full_unstemmed | Effects of palm oil derived tocotrienol rich fraction and vitamin e isomers on biomarkers of early atherogenesis in stimulated human umbilical vein endothelial cells |
| title_short | Effects of palm oil derived tocotrienol rich fraction and vitamin e isomers on biomarkers of early atherogenesis in stimulated human umbilical vein endothelial cells |
| title_sort | effects of palm oil derived tocotrienol rich fraction and vitamin e isomers on biomarkers of early atherogenesis in stimulated human umbilical vein endothelial cells |
| url | http://journalarticle.ukm.my/21432/ http://journalarticle.ukm.my/21432/ http://journalarticle.ukm.my/21432/1/M%2018.pdf |