Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells
Plant secondary metabolites and their derivatives play a significant role in anticancer drug therapy since they are effective against specific characteristics while reducing side effects. Dillapiole is a phenylpropanoid that holds several bioactivities like anti-fungal, anti-inflammatory, anti-leish...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/21415/ http://journalarticle.ukm.my/21415/1/M%205.pdf |
| _version_ | 1848815346466684928 |
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| author | Indah Mohd Amin, Nur Batrisyia Ruslan, Zulinda Ayu Zulkipli, Nurul Aili Zakaria, Mohd Taufiq Mat Jalil, Farizan Aris, |
| author_facet | Indah Mohd Amin, Nur Batrisyia Ruslan, Zulinda Ayu Zulkipli, Nurul Aili Zakaria, Mohd Taufiq Mat Jalil, Farizan Aris, |
| author_sort | Indah Mohd Amin, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Plant secondary metabolites and their derivatives play a significant role in anticancer drug therapy since they are effective against specific characteristics while reducing side effects. Dillapiole is a phenylpropanoid that holds several bioactivities like anti-fungal, anti-inflammatory, anti-leishmanial, and anti-cancer. This study aims to investigate the possible cytotoxic effect of dillapiole on human breast cancer, MCF-7 cells. Cells were cultured in complete RPMI media and incubated at standard culture conditions. After the cells reached 80% confluency, cells were treated with various concentrations (ranging from 0 μM to 150 μM) of dillapiole and tamoxifen as a positive control. Cells were later incubated at 48 and 72 h. Using WST-1 assay, the cytotoxic effect was determined for both incubation times. Results show tamoxifen inhibited the MCF-7 cells with the IC50 at 75.9 μM and 39.8 μM for 48 and 72 h respectively. Parallel with the positive control results, there was a significant cytotoxic effect of dillapiole against MCF-7 cells at 48- and 72-hr incubation with the IC50 at 92.1 μM and 63.1 μM respectively. Based on these results, dillapiole was cytotoxic against MCF-7 cells and its cytotoxic activity was both in a time and dose-dependent manner (<0.05). The morphological analysis supported the WST-1 assay. Our preliminary finding is in agreement with other previous studies, suggesting that dillapiole appears to be a promising anti-cancer agent and opens a wider possibility of downstream analysis on its underlying cytotoxicity mechanism. |
| first_indexed | 2025-11-15T00:48:31Z |
| format | Article |
| id | oai:generic.eprints.org:21415 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:48:31Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:214152023-04-03T04:56:16Z http://journalarticle.ukm.my/21415/ Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells Indah Mohd Amin, Nur Batrisyia Ruslan, Zulinda Ayu Zulkipli, Nurul Aili Zakaria, Mohd Taufiq Mat Jalil, Farizan Aris, Plant secondary metabolites and their derivatives play a significant role in anticancer drug therapy since they are effective against specific characteristics while reducing side effects. Dillapiole is a phenylpropanoid that holds several bioactivities like anti-fungal, anti-inflammatory, anti-leishmanial, and anti-cancer. This study aims to investigate the possible cytotoxic effect of dillapiole on human breast cancer, MCF-7 cells. Cells were cultured in complete RPMI media and incubated at standard culture conditions. After the cells reached 80% confluency, cells were treated with various concentrations (ranging from 0 μM to 150 μM) of dillapiole and tamoxifen as a positive control. Cells were later incubated at 48 and 72 h. Using WST-1 assay, the cytotoxic effect was determined for both incubation times. Results show tamoxifen inhibited the MCF-7 cells with the IC50 at 75.9 μM and 39.8 μM for 48 and 72 h respectively. Parallel with the positive control results, there was a significant cytotoxic effect of dillapiole against MCF-7 cells at 48- and 72-hr incubation with the IC50 at 92.1 μM and 63.1 μM respectively. Based on these results, dillapiole was cytotoxic against MCF-7 cells and its cytotoxic activity was both in a time and dose-dependent manner (<0.05). The morphological analysis supported the WST-1 assay. Our preliminary finding is in agreement with other previous studies, suggesting that dillapiole appears to be a promising anti-cancer agent and opens a wider possibility of downstream analysis on its underlying cytotoxicity mechanism. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21415/1/M%205.pdf Indah Mohd Amin, and Nur Batrisyia Ruslan, and Zulinda Ayu Zulkipli, and Nurul Aili Zakaria, and Mohd Taufiq Mat Jalil, and Farizan Aris, (2022) Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells. Malaysian Applied Biology, 51 (4). pp. 29-35. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
| spellingShingle | Indah Mohd Amin, Nur Batrisyia Ruslan, Zulinda Ayu Zulkipli, Nurul Aili Zakaria, Mohd Taufiq Mat Jalil, Farizan Aris, Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells |
| title | Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells |
| title_full | Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells |
| title_fullStr | Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells |
| title_full_unstemmed | Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells |
| title_short | Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells |
| title_sort | cytotoxic effect of dillapiole on human breast cancer mcf-7 cells |
| url | http://journalarticle.ukm.my/21415/ http://journalarticle.ukm.my/21415/ http://journalarticle.ukm.my/21415/1/M%205.pdf |