Fatty acid profiling and physiochemical characterization of Chlorella sorokiniana potentially used for biofuel production
Rising oil prices and climate change have resulted in more emphasis on research into renewable biofuels. In this study, different water samples were collected from local vicinities for the isolation of local isolates of microalgae to check their potential towards the production of biofuel by the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20461/ http://journalarticle.ukm.my/20461/1/15.pdf |
| _version_ | 1848815108871946240 |
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| author | Muhammad Ammar, Omer, Maria Aman, Sitwat Abdul Hameed, Abbas, Safdar Shaheen, Shabnam Abbas, Aiman Shakeel, Samina N |
| author_facet | Muhammad Ammar, Omer, Maria Aman, Sitwat Abdul Hameed, Abbas, Safdar Shaheen, Shabnam Abbas, Aiman Shakeel, Samina N |
| author_sort | Muhammad Ammar, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Rising oil prices and climate change have resulted in more emphasis on research into renewable biofuels. In this study,
different water samples were collected from local vicinities for the isolation of local isolates of microalgae to check their
potential towards the production of biofuel by the addition of different chemical substrates. Five different concentrations
of ascorbic acid and iron (III) chloride (0, 1, 2.5, 5 & 10 µM) are used as substrates. Microscopic analysis evaluated that
samples belong to genus Chlorella and further molecular identification showed that the samples are C. sorokiniana.
Among all the concentrations of ascorbic acid 2.5 µM is most effective against the C. sorokiniana strain 1 (Safari Wildlife
Park, Lahore) and C. sorokiniana strain 2 (Bahria Town, Lahore) while C. sorokiniana Strain 3 (SukhChane Society,
Lahore) responded at 2.5 & 5 µM in term of biomass production. FeCl3
(2.5 µM) is effective against C. sorokiniana strain
1 while the growth of C. sorokiniana strain 2 and C. sorokiniana Strain 3 is inhibited. Lipid content analysis showed
that only the C. sorokiniana strain 1 shows effective results at 1 & 2.5 µM of ascorbic acid and FeCl3
, respectively.
Those concentrations which give the significant results of lipid production were preceded for fatty acid profiling. Results
indicate that the C. sorokiniana strain 1 can be considered as a source of alpha-linolenic acid; the basic constituent of
biofuel production. In this study, it is concluded that C. sorokiniana strain 1 is useful for the production of environment
friendly biofuel. |
| first_indexed | 2025-11-15T00:44:45Z |
| format | Article |
| id | oai:generic.eprints.org:20461 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:44:45Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:204612022-11-10T07:14:11Z http://journalarticle.ukm.my/20461/ Fatty acid profiling and physiochemical characterization of Chlorella sorokiniana potentially used for biofuel production Muhammad Ammar, Omer, Maria Aman, Sitwat Abdul Hameed, Abbas, Safdar Shaheen, Shabnam Abbas, Aiman Shakeel, Samina N Rising oil prices and climate change have resulted in more emphasis on research into renewable biofuels. In this study, different water samples were collected from local vicinities for the isolation of local isolates of microalgae to check their potential towards the production of biofuel by the addition of different chemical substrates. Five different concentrations of ascorbic acid and iron (III) chloride (0, 1, 2.5, 5 & 10 µM) are used as substrates. Microscopic analysis evaluated that samples belong to genus Chlorella and further molecular identification showed that the samples are C. sorokiniana. Among all the concentrations of ascorbic acid 2.5 µM is most effective against the C. sorokiniana strain 1 (Safari Wildlife Park, Lahore) and C. sorokiniana strain 2 (Bahria Town, Lahore) while C. sorokiniana Strain 3 (SukhChane Society, Lahore) responded at 2.5 & 5 µM in term of biomass production. FeCl3 (2.5 µM) is effective against C. sorokiniana strain 1 while the growth of C. sorokiniana strain 2 and C. sorokiniana Strain 3 is inhibited. Lipid content analysis showed that only the C. sorokiniana strain 1 shows effective results at 1 & 2.5 µM of ascorbic acid and FeCl3 , respectively. Those concentrations which give the significant results of lipid production were preceded for fatty acid profiling. Results indicate that the C. sorokiniana strain 1 can be considered as a source of alpha-linolenic acid; the basic constituent of biofuel production. In this study, it is concluded that C. sorokiniana strain 1 is useful for the production of environment friendly biofuel. Penerbit Universiti Kebangsaan Malaysia 2022-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20461/1/15.pdf Muhammad Ammar, and Omer, Maria and Aman, Sitwat and Abdul Hameed, and Abbas, Safdar and Shaheen, Shabnam and Abbas, Aiman and Shakeel, Samina N (2022) Fatty acid profiling and physiochemical characterization of Chlorella sorokiniana potentially used for biofuel production. Sains Malaysiana, 51 (8). pp. 2547-2557. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil8_2022/KandunganJilid51Bil8_2022.html |
| spellingShingle | Muhammad Ammar, Omer, Maria Aman, Sitwat Abdul Hameed, Abbas, Safdar Shaheen, Shabnam Abbas, Aiman Shakeel, Samina N Fatty acid profiling and physiochemical characterization of Chlorella sorokiniana potentially used for biofuel production |
| title | Fatty acid profiling and physiochemical characterization of Chlorella sorokiniana potentially used for biofuel production |
| title_full | Fatty acid profiling and physiochemical characterization of Chlorella sorokiniana potentially used for biofuel production |
| title_fullStr | Fatty acid profiling and physiochemical characterization of Chlorella sorokiniana potentially used for biofuel production |
| title_full_unstemmed | Fatty acid profiling and physiochemical characterization of Chlorella sorokiniana potentially used for biofuel production |
| title_short | Fatty acid profiling and physiochemical characterization of Chlorella sorokiniana potentially used for biofuel production |
| title_sort | fatty acid profiling and physiochemical characterization of chlorella sorokiniana potentially used for biofuel production |
| url | http://journalarticle.ukm.my/20461/ http://journalarticle.ukm.my/20461/ http://journalarticle.ukm.my/20461/1/15.pdf |