Characterisation of Tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers
Tetrastigma rafflesiae (Miq.) Planch. is a climbing plant species that is known for its unique relationship with holoparasitic plants of Rafflesiaceae. Knowledge on the mitochondrial genes of this species may contribute towards the development of molecular approaches for species identification....
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20454/ http://journalarticle.ukm.my/20454/1/8.pdf |
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| author | Mohamad Hafizzudin-Fedeli, Mohd-Faizal Abu-Bakar, Mohd-Noor Mat-Isa, A. Latiff, Mohd Firdaus-Raih, Wan, Kiew Lian |
| author_facet | Mohamad Hafizzudin-Fedeli, Mohd-Faizal Abu-Bakar, Mohd-Noor Mat-Isa, A. Latiff, Mohd Firdaus-Raih, Wan, Kiew Lian |
| author_sort | Mohamad Hafizzudin-Fedeli, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Tetrastigma rafflesiae (Miq.) Planch. is a climbing plant species that is known for its unique relationship with
holoparasitic plants of Rafflesiaceae. Knowledge on the mitochondrial genes of this species may contribute towards the
development of molecular approaches for species identification. This study aimes to identify and characterise genes from
the T. rafflesiae mitochondrial genome (mitogenome) and assess their potential to discriminate different Tetrastigma
species. Mitochondrial-specific sequences were first selected by mapping T. rafflesiae whole-genome sequences to
mitogenomes from several reference plant species. De novo assembly of these selected sequences produced a T. rafflesiae
mitogenome with a size of 336 kb. Gene annotation revealed that the T. rafflesiae mitogenome contains at least
40 protein coding genes, 20 tRNAs and two rRNAs. Phylogenetic analysis using several mitochondrial genes, namely
ccmB, cob, matR, nad6 and rps3 was able to differentiate T. rafflesiae from three other Tetrastigma species, indicating the
potential of these genes as species-specific sequence markers. These findings supplement additional genetic information
on T. rafflesiae and may aid in the effort of species classification and conservation. |
| first_indexed | 2025-11-15T00:44:43Z |
| format | Article |
| id | oai:generic.eprints.org:20454 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:44:43Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:204542022-11-10T07:06:41Z http://journalarticle.ukm.my/20454/ Characterisation of Tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers Mohamad Hafizzudin-Fedeli, Mohd-Faizal Abu-Bakar, Mohd-Noor Mat-Isa, A. Latiff, Mohd Firdaus-Raih, Wan, Kiew Lian Tetrastigma rafflesiae (Miq.) Planch. is a climbing plant species that is known for its unique relationship with holoparasitic plants of Rafflesiaceae. Knowledge on the mitochondrial genes of this species may contribute towards the development of molecular approaches for species identification. This study aimes to identify and characterise genes from the T. rafflesiae mitochondrial genome (mitogenome) and assess their potential to discriminate different Tetrastigma species. Mitochondrial-specific sequences were first selected by mapping T. rafflesiae whole-genome sequences to mitogenomes from several reference plant species. De novo assembly of these selected sequences produced a T. rafflesiae mitogenome with a size of 336 kb. Gene annotation revealed that the T. rafflesiae mitogenome contains at least 40 protein coding genes, 20 tRNAs and two rRNAs. Phylogenetic analysis using several mitochondrial genes, namely ccmB, cob, matR, nad6 and rps3 was able to differentiate T. rafflesiae from three other Tetrastigma species, indicating the potential of these genes as species-specific sequence markers. These findings supplement additional genetic information on T. rafflesiae and may aid in the effort of species classification and conservation. Penerbit Universiti Kebangsaan Malaysia 2022-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20454/1/8.pdf Mohamad Hafizzudin-Fedeli, and Mohd-Faizal Abu-Bakar, and Mohd-Noor Mat-Isa, and A. Latiff, and Mohd Firdaus-Raih, and Wan, Kiew Lian (2022) Characterisation of Tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers. Sains Malaysiana, 51 (8). pp. 2435-2447. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil8_2022/KandunganJilid51Bil8_2022.html |
| spellingShingle | Mohamad Hafizzudin-Fedeli, Mohd-Faizal Abu-Bakar, Mohd-Noor Mat-Isa, A. Latiff, Mohd Firdaus-Raih, Wan, Kiew Lian Characterisation of Tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers |
| title | Characterisation of Tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers |
| title_full | Characterisation of Tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers |
| title_fullStr | Characterisation of Tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers |
| title_full_unstemmed | Characterisation of Tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers |
| title_short | Characterisation of Tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers |
| title_sort | characterisation of tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers |
| url | http://journalarticle.ukm.my/20454/ http://journalarticle.ukm.my/20454/ http://journalarticle.ukm.my/20454/1/8.pdf |