Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts
Extracts from Saccharomyces cerevisiae are incorporated in a lot of cosmetic products on the market, but the benefits of the extracts lack scientific reports. We tested the toxicity and anti-senescent activity of an extract from S. cerevisiae on an in vitro model, the human dermal fibroblast (HD...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20452/ http://journalarticle.ukm.my/20452/1/6.pdf |
| _version_ | 1848815106476998656 |
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| author | Khaizurin Tajul Arifin, Noor Ikhwan Shansuddin, Norwahidah Abdul Karim, Suzana Makpol, |
| author_facet | Khaizurin Tajul Arifin, Noor Ikhwan Shansuddin, Norwahidah Abdul Karim, Suzana Makpol, |
| author_sort | Khaizurin Tajul Arifin, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Extracts from Saccharomyces cerevisiae are incorporated in a lot of cosmetic products on the market, but the benefits
of the extracts lack scientific reports. We tested the toxicity and anti-senescent activity of an extract from S. cerevisiae
on an in vitro model, the human dermal fibroblast (HDF) cell culture. We chronicled the development of the extraction
method and the subsequent biochemical assays. We used two extraction methods which were hot water extraction
and rapid spin. The optimum duration and growth phase to harvest S. cerevisiae were determined by 2,2-diphenyl-1-
picrylhydrazyl (DPPH) assay, which also proved that the extracts exhibited antioxidant activity. Hot water extract showed
a higher antioxidant activity, and not toxic to HDF. When subjected to senescence-associated β-galactosidase (SA-β-al) assay, the hot water extract significantly reduced the expression of SA-β-Gal in pre-senescent (passage 20, 30 <
population doubling < 40) and senescent (passage 30, population doubling > 50) HDF. In conclusion, S. cerevisiae hot
water extract possessed antioxidant activity by scavenging DPPH, and anti-senescent activity by reducing the expression
of SA-β-Gal in pre-senescent and senescent HDF. |
| first_indexed | 2025-11-15T00:44:42Z |
| format | Article |
| id | oai:generic.eprints.org:20452 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:44:42Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:204522022-11-10T07:05:15Z http://journalarticle.ukm.my/20452/ Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts Khaizurin Tajul Arifin, Noor Ikhwan Shansuddin, Norwahidah Abdul Karim, Suzana Makpol, Extracts from Saccharomyces cerevisiae are incorporated in a lot of cosmetic products on the market, but the benefits of the extracts lack scientific reports. We tested the toxicity and anti-senescent activity of an extract from S. cerevisiae on an in vitro model, the human dermal fibroblast (HDF) cell culture. We chronicled the development of the extraction method and the subsequent biochemical assays. We used two extraction methods which were hot water extraction and rapid spin. The optimum duration and growth phase to harvest S. cerevisiae were determined by 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay, which also proved that the extracts exhibited antioxidant activity. Hot water extract showed a higher antioxidant activity, and not toxic to HDF. When subjected to senescence-associated β-galactosidase (SA-β-al) assay, the hot water extract significantly reduced the expression of SA-β-Gal in pre-senescent (passage 20, 30 < population doubling < 40) and senescent (passage 30, population doubling > 50) HDF. In conclusion, S. cerevisiae hot water extract possessed antioxidant activity by scavenging DPPH, and anti-senescent activity by reducing the expression of SA-β-Gal in pre-senescent and senescent HDF. Penerbit Universiti Kebangsaan Malaysia 2022-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20452/1/6.pdf Khaizurin Tajul Arifin, and Noor Ikhwan Shansuddin, and Norwahidah Abdul Karim, and Suzana Makpol, (2022) Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts. Sains Malaysiana, 51 (8). pp. 2415-2424. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil8_2022/KandunganJilid51Bil8_2022.html |
| spellingShingle | Khaizurin Tajul Arifin, Noor Ikhwan Shansuddin, Norwahidah Abdul Karim, Suzana Makpol, Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts |
| title | Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts |
| title_full | Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts |
| title_fullStr | Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts |
| title_full_unstemmed | Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts |
| title_short | Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts |
| title_sort | hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-galactosidase (sa-β-gal) in human dermal fibroblasts |
| url | http://journalarticle.ukm.my/20452/ http://journalarticle.ukm.my/20452/ http://journalarticle.ukm.my/20452/1/6.pdf |