Transient or permanent inhabitants : cultured internal microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp.
Monogeneans, a class of parasitic platyhelminthes, are usually found on the surface of fish. When it feeds on its host, it harbours bacteria, which can survive in its gut. Occasionally, the monogeneans may cause damages on fish tissue while feeding and may allow secondary infection. The present...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20447/ http://journalarticle.ukm.my/20447/1/1.pdf |
| _version_ | 1848815105229193216 |
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| author | Ong, Zhi-Zhang Chong, Yeak-Heng Wong, Wey-Lim |
| author_facet | Ong, Zhi-Zhang Chong, Yeak-Heng Wong, Wey-Lim |
| author_sort | Ong, Zhi-Zhang |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Monogeneans, a class of parasitic platyhelminthes, are usually found on the surface of fish. When it feeds on its host, it
harbours bacteria, which can survive in its gut. Occasionally, the monogeneans may cause damages on fish tissue while
feeding and may allow secondary infection. The present study aimed to isolate and identify culturable bacteria obtained
within the gill monogeneans, Cichlidogyrus thurstonae and Scutogyrus longicornis, and gill surface of the tilapia fish,
Oreochromis sp. based on 16S ribosomal RNA gene sequencing. Monogeneans were isolated from the fish gill filaments
and surface disinfected using 70% ethanol before squashed aseptically on Luria Bertani (LB) agar to isolate the internal
microbiota. A total of five bacteria species, namely Burkholderia sp., En terobacter h o rmaechei, Enterobacter sp.,
Ochrobactrum intermedium and Pantoea sp., were found within C. thurstonae, whilst a total of eight bacteria species,
namely Burkholderia contaminans, Pantoea dispersa, Sphingomonas yabuuchiae, Rhizobium pusense, O. intermedium,
Acinetobacter bereziniae, Escherichia hermannii, and Staphylococcus saprophyticus, were found within S. longicornis
in which B. contaminans, P. dispersa, and S. yabuuchiae were also found on the surface of the tilapia fish gill filaments.
Enterobacter bugandensis and Acinetobacter pittii were found solely on the surface of the gill filaments. These bacteria
are also found in the environment and some of them are believed to be pathogenic to fish. We suggest that monogeneans
may serve as potential bacteria reservoirs, which facilitate the transmission of bacteria. |
| first_indexed | 2025-11-15T00:44:41Z |
| format | Article |
| id | oai:generic.eprints.org:20447 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:44:41Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:204472022-11-10T06:59:37Z http://journalarticle.ukm.my/20447/ Transient or permanent inhabitants : cultured internal microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp. Ong, Zhi-Zhang Chong, Yeak-Heng Wong, Wey-Lim Monogeneans, a class of parasitic platyhelminthes, are usually found on the surface of fish. When it feeds on its host, it harbours bacteria, which can survive in its gut. Occasionally, the monogeneans may cause damages on fish tissue while feeding and may allow secondary infection. The present study aimed to isolate and identify culturable bacteria obtained within the gill monogeneans, Cichlidogyrus thurstonae and Scutogyrus longicornis, and gill surface of the tilapia fish, Oreochromis sp. based on 16S ribosomal RNA gene sequencing. Monogeneans were isolated from the fish gill filaments and surface disinfected using 70% ethanol before squashed aseptically on Luria Bertani (LB) agar to isolate the internal microbiota. A total of five bacteria species, namely Burkholderia sp., En terobacter h o rmaechei, Enterobacter sp., Ochrobactrum intermedium and Pantoea sp., were found within C. thurstonae, whilst a total of eight bacteria species, namely Burkholderia contaminans, Pantoea dispersa, Sphingomonas yabuuchiae, Rhizobium pusense, O. intermedium, Acinetobacter bereziniae, Escherichia hermannii, and Staphylococcus saprophyticus, were found within S. longicornis in which B. contaminans, P. dispersa, and S. yabuuchiae were also found on the surface of the tilapia fish gill filaments. Enterobacter bugandensis and Acinetobacter pittii were found solely on the surface of the gill filaments. These bacteria are also found in the environment and some of them are believed to be pathogenic to fish. We suggest that monogeneans may serve as potential bacteria reservoirs, which facilitate the transmission of bacteria. Penerbit Universiti Kebangsaan Malaysia 2022-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20447/1/1.pdf Ong, Zhi-Zhang and Chong, Yeak-Heng and Wong, Wey-Lim (2022) Transient or permanent inhabitants : cultured internal microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp. Sains Malaysiana, 51 (8). pp. 2339-2349. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil8_2022/KandunganJilid51Bil8_2022.html |
| spellingShingle | Ong, Zhi-Zhang Chong, Yeak-Heng Wong, Wey-Lim Transient or permanent inhabitants : cultured internal microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp. |
| title | Transient or permanent inhabitants : cultured internal microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp. |
| title_full | Transient or permanent inhabitants : cultured internal microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp. |
| title_fullStr | Transient or permanent inhabitants : cultured internal microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp. |
| title_full_unstemmed | Transient or permanent inhabitants : cultured internal microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp. |
| title_short | Transient or permanent inhabitants : cultured internal microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp. |
| title_sort | transient or permanent inhabitants : cultured internal microbiota of cichlidogyrus thurstonae and scutogyrus longicornis (monogenea: ancyrocephalidae) from oreochromis sp. |
| url | http://journalarticle.ukm.my/20447/ http://journalarticle.ukm.my/20447/ http://journalarticle.ukm.my/20447/1/1.pdf |