Molecular incidence of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum in dissimilar kinds of raw and pasteurized milk samples of naturally infected animal species
Milk of naturally infected animal species is considered an essential reservoir of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum. An extant survey was performed to measure the T. gondii, N. caninum, and C. parvum incidence and periodic frequency amongst milk samples. One-thousand an...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20237/ http://journalarticle.ukm.my/20237/1/10.pdf |
| _version_ | 1848815050472554496 |
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| author | Abdollahzadeh, Farzad Firouzabadi, Mohammad Sadegh Safaee Shomali, Romina Rashid |
| author_facet | Abdollahzadeh, Farzad Firouzabadi, Mohammad Sadegh Safaee Shomali, Romina Rashid |
| author_sort | Abdollahzadeh, Farzad |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Milk of naturally infected animal species is considered an essential reservoir of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum. An extant survey was performed to measure the T. gondii, N. caninum, and C. parvum incidence and periodic frequency amongst milk samples. One-thousand and one-hundred and sixty raw and 400 pasteurized milk samples were collected. Milk samples were used for DNA extraction. The nested-polymerase chain reaction was applied to diagnose B1, NP, and hsp70 genes of the T. gondii, N. caninum, and C. parvum, respectively. The total incidence of T. gondii, N. caninum, and C. parvum in examined milk samples were 2.69%, 10.51%, and 2.94%, respectively. Co-contamination of examined milk samples with all three protozoa was 0.38%. T. gondii had the highest distribution amongst raw sheep (5.00%) milk samples, while N. caninum, and C. parvum had the highest distribution in raw buffalo (23.68% and 6.31%, respectively) milk samples. Marked periodicity with the higher distribution of all protozoa among raw milk samples collected during autumn and summer seasons were found (P <0.05). Raw milk of animal species and pasteurized cow milk may be reservoirs of N. caninum, T. gondii, and C. parvum. Accurate monitoring of raw and pasteurized milk, particularly in autumn and summer seasons, can diminish the risk of human and animal toxoplasmosis, cryptosporidiosis, and neosporosis. However, further investigations are essential to understand the exact epidemiological aspects and other risk factors associated with the N. caninum, C. parvum, and T. gondii presence in raw and pasteurized milk. |
| first_indexed | 2025-11-15T00:43:49Z |
| format | Article |
| id | oai:generic.eprints.org:20237 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:43:49Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:202372022-10-25T07:27:53Z http://journalarticle.ukm.my/20237/ Molecular incidence of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum in dissimilar kinds of raw and pasteurized milk samples of naturally infected animal species Abdollahzadeh, Farzad Firouzabadi, Mohammad Sadegh Safaee Shomali, Romina Rashid Milk of naturally infected animal species is considered an essential reservoir of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum. An extant survey was performed to measure the T. gondii, N. caninum, and C. parvum incidence and periodic frequency amongst milk samples. One-thousand and one-hundred and sixty raw and 400 pasteurized milk samples were collected. Milk samples were used for DNA extraction. The nested-polymerase chain reaction was applied to diagnose B1, NP, and hsp70 genes of the T. gondii, N. caninum, and C. parvum, respectively. The total incidence of T. gondii, N. caninum, and C. parvum in examined milk samples were 2.69%, 10.51%, and 2.94%, respectively. Co-contamination of examined milk samples with all three protozoa was 0.38%. T. gondii had the highest distribution amongst raw sheep (5.00%) milk samples, while N. caninum, and C. parvum had the highest distribution in raw buffalo (23.68% and 6.31%, respectively) milk samples. Marked periodicity with the higher distribution of all protozoa among raw milk samples collected during autumn and summer seasons were found (P <0.05). Raw milk of animal species and pasteurized cow milk may be reservoirs of N. caninum, T. gondii, and C. parvum. Accurate monitoring of raw and pasteurized milk, particularly in autumn and summer seasons, can diminish the risk of human and animal toxoplasmosis, cryptosporidiosis, and neosporosis. However, further investigations are essential to understand the exact epidemiological aspects and other risk factors associated with the N. caninum, C. parvum, and T. gondii presence in raw and pasteurized milk. Penerbit Universiti Kebangsaan Malaysia 2022-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20237/1/10.pdf Abdollahzadeh, Farzad and Firouzabadi, Mohammad Sadegh Safaee and Shomali, Romina Rashid (2022) Molecular incidence of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum in dissimilar kinds of raw and pasteurized milk samples of naturally infected animal species. Sains Malaysiana, 51 (7). pp. 2061-2071. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil7_2022/KandunganJilid51Bil7_2022.html |
| spellingShingle | Abdollahzadeh, Farzad Firouzabadi, Mohammad Sadegh Safaee Shomali, Romina Rashid Molecular incidence of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum in dissimilar kinds of raw and pasteurized milk samples of naturally infected animal species |
| title | Molecular incidence of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum in dissimilar kinds of raw and pasteurized milk samples of naturally infected animal species |
| title_full | Molecular incidence of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum in dissimilar kinds of raw and pasteurized milk samples of naturally infected animal species |
| title_fullStr | Molecular incidence of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum in dissimilar kinds of raw and pasteurized milk samples of naturally infected animal species |
| title_full_unstemmed | Molecular incidence of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum in dissimilar kinds of raw and pasteurized milk samples of naturally infected animal species |
| title_short | Molecular incidence of Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum in dissimilar kinds of raw and pasteurized milk samples of naturally infected animal species |
| title_sort | molecular incidence of toxoplasma gondii, neospora caninum, and cryptosporidium parvum in dissimilar kinds of raw and pasteurized milk samples of naturally infected animal species |
| url | http://journalarticle.ukm.my/20237/ http://journalarticle.ukm.my/20237/ http://journalarticle.ukm.my/20237/1/10.pdf |