Comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee
Culture expanded chondrocytes isolated from non-load bearing region of osteoarthritic (OA) joint has been used to construct tissue engineered cartilage for treatment purposes. The aim of the study was to compare the histological properties of the cartilage tissue and morphological properties of t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pusat Perubatan Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/20201/ http://journalarticle.ukm.my/20201/1/8_ms0168_pdf_18024.pdf |
| _version_ | 1848815040750157824 |
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| author | Nurzazlin BZN, Shamsul BS, Yahya NHM, Ruszymah BHI, Abdul Rani R, Shiplu Roy Chowdhury, |
| author_facet | Nurzazlin BZN, Shamsul BS, Yahya NHM, Ruszymah BHI, Abdul Rani R, Shiplu Roy Chowdhury, |
| author_sort | Nurzazlin BZN, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Culture expanded chondrocytes isolated from non-load bearing region of
osteoarthritic (OA) joint has been used to construct tissue engineered cartilage
for treatment purposes. The aim of the study was to compare the histological
properties of the cartilage tissue and morphological properties of the chondrocytes
isolated from less and severely affected OA knee. Human articular cartilage
was obtained as redundant tissue from consented patients with late-stage OA
undergoing total knee replacement surgery at Universiti Kebangsaan Malaysia
Medical Centre (UKMMC). Articular cartilage was graded according to Dougados
and Osteoarthritis Research Society International (OARSI) classification. Articular
cartilage was classified into less affected (LA; Grade 0-1) and severely affected (SA;
Grade 2-3). Cartilage tissue from less and severely affected region was stained with
Safranin O staining. Isolated chondrocytes from each group were cultured until
passage 4 (P4). Their growth patterns, cell areas, and circularity were compared.
LA-cartilage tissue shows uniform spread of safranin O staining indicating intact
extracellular matrix (ECM) component. However, SA-cartilage shows significant
reduction and unstable staining due to its degraded ECM. LA-chondrocytes
showed an aggregated growth compared to SA-chondrocyte that remains
monolayer. Moreover, LA-chondrocytes have significantly higher cell area with
wider spreading at passage 0 and 4 compared to SA-chondrocytes. It was also
found that chondrocyte circularity increased with passage, and circularity of LAchondrocytes
was significantly higher than that of the SA-chondrocytes at passage
3. This study demonstrated the considerable difference in the cellular properties
for less and severely affected chondrocytes and implication of these differences in
cell-based therapy needed to be explored. |
| first_indexed | 2025-11-15T00:43:40Z |
| format | Article |
| id | oai:generic.eprints.org:20201 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:43:40Z |
| publishDate | 2018 |
| publisher | Pusat Perubatan Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:202012022-10-21T08:18:27Z http://journalarticle.ukm.my/20201/ Comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee Nurzazlin BZN, Shamsul BS, Yahya NHM, Ruszymah BHI, Abdul Rani R, Shiplu Roy Chowdhury, Culture expanded chondrocytes isolated from non-load bearing region of osteoarthritic (OA) joint has been used to construct tissue engineered cartilage for treatment purposes. The aim of the study was to compare the histological properties of the cartilage tissue and morphological properties of the chondrocytes isolated from less and severely affected OA knee. Human articular cartilage was obtained as redundant tissue from consented patients with late-stage OA undergoing total knee replacement surgery at Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Articular cartilage was graded according to Dougados and Osteoarthritis Research Society International (OARSI) classification. Articular cartilage was classified into less affected (LA; Grade 0-1) and severely affected (SA; Grade 2-3). Cartilage tissue from less and severely affected region was stained with Safranin O staining. Isolated chondrocytes from each group were cultured until passage 4 (P4). Their growth patterns, cell areas, and circularity were compared. LA-cartilage tissue shows uniform spread of safranin O staining indicating intact extracellular matrix (ECM) component. However, SA-cartilage shows significant reduction and unstable staining due to its degraded ECM. LA-chondrocytes showed an aggregated growth compared to SA-chondrocyte that remains monolayer. Moreover, LA-chondrocytes have significantly higher cell area with wider spreading at passage 0 and 4 compared to SA-chondrocytes. It was also found that chondrocyte circularity increased with passage, and circularity of LAchondrocytes was significantly higher than that of the SA-chondrocytes at passage 3. This study demonstrated the considerable difference in the cellular properties for less and severely affected chondrocytes and implication of these differences in cell-based therapy needed to be explored. Pusat Perubatan Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20201/1/8_ms0168_pdf_18024.pdf Nurzazlin BZN, and Shamsul BS, and Yahya NHM, and Ruszymah BHI, and Abdul Rani R, and Shiplu Roy Chowdhury, (2018) Comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee. Medicine & Health, 13 (1). pp. 77-87. ISSN 2289-5728 https://www.medicineandhealthukm.com/toc/13/1 |
| spellingShingle | Nurzazlin BZN, Shamsul BS, Yahya NHM, Ruszymah BHI, Abdul Rani R, Shiplu Roy Chowdhury, Comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee |
| title | Comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee |
| title_full | Comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee |
| title_fullStr | Comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee |
| title_full_unstemmed | Comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee |
| title_short | Comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee |
| title_sort | comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee |
| url | http://journalarticle.ukm.my/20201/ http://journalarticle.ukm.my/20201/ http://journalarticle.ukm.my/20201/1/8_ms0168_pdf_18024.pdf |