Monitoring blood cholinesterase activity of farmworkers : in vitro inhibition by diphenhydramine and carbaryl
Plasma and erythrocyte cholinesterase (ChE) activities of male farm workers exposed to pesticides during their routine work were lower than those of unexposed control subjects by 14 and 4%, respectively. Diphenhydramine and Carbaryl inhibited plasma and erythrocyte ChE activities in vitro in pestici...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20186/ http://journalarticle.ukm.my/20186/1/%2BMAB%2B2204.pdf |
| _version_ | 1848815036641837056 |
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| author | Mohammed, Ammar Ahmed Mohammad, Fouad Kasim |
| author_facet | Mohammed, Ammar Ahmed Mohammad, Fouad Kasim |
| author_sort | Mohammed, Ammar Ahmed |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Plasma and erythrocyte cholinesterase (ChE) activities of male farm workers exposed to pesticides during their routine work were lower than those of unexposed control subjects by 14 and 4%, respectively. Diphenhydramine and Carbaryl inhibited plasma and erythrocyte ChE activities in vitro in pesticide-exposed and unexposed subjects. The percentages of in vitro ChE inhibition induced by carbaryl in the plasma and erythrocytes of unexposed controls ranged between 47-85% and 19-47%, respectively, whereas they were 35-60% and 3-12% in the pesticide-exposed group, respectively. In vitro pretreatment of plasma and erythrocyte ChE with diphenhydramine (20 μM) significantly reduced the inhibitory effect of carbaryl (10 μM) on them by 18% and 10%, respectively. In conclusion, subjects exposed to pesticides during their routine work in agriculture are at risk of reduced blood ChE activity. Diphenhydramine appeared to partially protect blood ChE in vitro from additional carbaryl-induced enzyme inhibition in both pesticide-exposed and unexposed subjects. |
| first_indexed | 2025-11-15T00:43:36Z |
| format | Article |
| id | oai:generic.eprints.org:20186 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:43:36Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:201862022-10-21T04:06:28Z http://journalarticle.ukm.my/20186/ Monitoring blood cholinesterase activity of farmworkers : in vitro inhibition by diphenhydramine and carbaryl Mohammed, Ammar Ahmed Mohammad, Fouad Kasim Plasma and erythrocyte cholinesterase (ChE) activities of male farm workers exposed to pesticides during their routine work were lower than those of unexposed control subjects by 14 and 4%, respectively. Diphenhydramine and Carbaryl inhibited plasma and erythrocyte ChE activities in vitro in pesticide-exposed and unexposed subjects. The percentages of in vitro ChE inhibition induced by carbaryl in the plasma and erythrocytes of unexposed controls ranged between 47-85% and 19-47%, respectively, whereas they were 35-60% and 3-12% in the pesticide-exposed group, respectively. In vitro pretreatment of plasma and erythrocyte ChE with diphenhydramine (20 μM) significantly reduced the inhibitory effect of carbaryl (10 μM) on them by 18% and 10%, respectively. In conclusion, subjects exposed to pesticides during their routine work in agriculture are at risk of reduced blood ChE activity. Diphenhydramine appeared to partially protect blood ChE in vitro from additional carbaryl-induced enzyme inhibition in both pesticide-exposed and unexposed subjects. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20186/1/%2BMAB%2B2204.pdf Mohammed, Ammar Ahmed and Mohammad, Fouad Kasim (2022) Monitoring blood cholinesterase activity of farmworkers : in vitro inhibition by diphenhydramine and carbaryl. Malaysian Applied Biology, 51 (2). pp. 23-32. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/45 |
| spellingShingle | Mohammed, Ammar Ahmed Mohammad, Fouad Kasim Monitoring blood cholinesterase activity of farmworkers : in vitro inhibition by diphenhydramine and carbaryl |
| title | Monitoring blood cholinesterase activity of farmworkers : in vitro inhibition by diphenhydramine and carbaryl |
| title_full | Monitoring blood cholinesterase activity of farmworkers : in vitro inhibition by diphenhydramine and carbaryl |
| title_fullStr | Monitoring blood cholinesterase activity of farmworkers : in vitro inhibition by diphenhydramine and carbaryl |
| title_full_unstemmed | Monitoring blood cholinesterase activity of farmworkers : in vitro inhibition by diphenhydramine and carbaryl |
| title_short | Monitoring blood cholinesterase activity of farmworkers : in vitro inhibition by diphenhydramine and carbaryl |
| title_sort | monitoring blood cholinesterase activity of farmworkers : in vitro inhibition by diphenhydramine and carbaryl |
| url | http://journalarticle.ukm.my/20186/ http://journalarticle.ukm.my/20186/ http://journalarticle.ukm.my/20186/1/%2BMAB%2B2204.pdf |