Quantitative HPLC and FTIR-based metabolomics for clustering Centella asiatica cultivation ages and evaluation of their radical scavenging activity
Centella asiatica is a medicinal plant used to treat stomachache, cough, sore throat, blood booster, and skin diseases. The difference in harvesting time is among the factors that can significantly affect the chemical composition of C. asiatica. The purpose of this study was to evaluate the differen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/19752/ http://journalarticle.ukm.my/19752/1/15.pdf |
| _version_ | 1848814922253729792 |
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| author | Rafi, Mohamad Anastasia Grandivoriana Nomi, Dewi Anggraini Septaningsih, Heryanto, Rudi Sastia Prama Putri, |
| author_facet | Rafi, Mohamad Anastasia Grandivoriana Nomi, Dewi Anggraini Septaningsih, Heryanto, Rudi Sastia Prama Putri, |
| author_sort | Rafi, Mohamad |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Centella asiatica is a medicinal plant used to treat stomachache, cough, sore throat, blood booster, and skin diseases. The difference in harvesting time is among the factors that can significantly affect the chemical composition of C. asiatica. The purpose of this study was to evaluate the difference of C. asiatica cultivation ages through a metabolomics (targeted and untargeted) analysis using FTIR spectra, HPLC analysis of four main components, and evaluation of its radical scavenging activity. The results showed that differences in cultivation ages affected the chemical composition of C. asiatica. It was shown by the FTIR spectrum indicating the vibration of several functional groups such as OH, C-H, C=O, C=C, C-O, C-N, C-O phenol, and alkyl halides with differences in their intensities. The results of the HPLC showed that C. asiatica harvested after four months post-planting (MPP) had the highest content of madecassoside, asiaticoside, madecassic acid, and asiatic acid, respectively. A principal component analysis (PCA) was carried out to clustering C. asiatica based on its cultivation ages. The PCA results showed that each sample could be grouped with a total variance of the first two principal components (PC) using peak are of the four main components analyzed by HPLC and absorbance at wavenumber 400-4000 cm-1 from FTIR spectra were 98% and 95%, respectively. The radical scavenging activity demonstrated that the 4 MPP had the highest inhibition of about 53.81±0.92 %. So, C. asiatica at 4 MPP gave optimum level four main metabolite content and radical scavenging activity. |
| first_indexed | 2025-11-15T00:41:47Z |
| format | Article |
| id | oai:generic.eprints.org:19752 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:41:47Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:197522022-09-19T06:57:04Z http://journalarticle.ukm.my/19752/ Quantitative HPLC and FTIR-based metabolomics for clustering Centella asiatica cultivation ages and evaluation of their radical scavenging activity Rafi, Mohamad Anastasia Grandivoriana Nomi, Dewi Anggraini Septaningsih, Heryanto, Rudi Sastia Prama Putri, Centella asiatica is a medicinal plant used to treat stomachache, cough, sore throat, blood booster, and skin diseases. The difference in harvesting time is among the factors that can significantly affect the chemical composition of C. asiatica. The purpose of this study was to evaluate the difference of C. asiatica cultivation ages through a metabolomics (targeted and untargeted) analysis using FTIR spectra, HPLC analysis of four main components, and evaluation of its radical scavenging activity. The results showed that differences in cultivation ages affected the chemical composition of C. asiatica. It was shown by the FTIR spectrum indicating the vibration of several functional groups such as OH, C-H, C=O, C=C, C-O, C-N, C-O phenol, and alkyl halides with differences in their intensities. The results of the HPLC showed that C. asiatica harvested after four months post-planting (MPP) had the highest content of madecassoside, asiaticoside, madecassic acid, and asiatic acid, respectively. A principal component analysis (PCA) was carried out to clustering C. asiatica based on its cultivation ages. The PCA results showed that each sample could be grouped with a total variance of the first two principal components (PC) using peak are of the four main components analyzed by HPLC and absorbance at wavenumber 400-4000 cm-1 from FTIR spectra were 98% and 95%, respectively. The radical scavenging activity demonstrated that the 4 MPP had the highest inhibition of about 53.81±0.92 %. So, C. asiatica at 4 MPP gave optimum level four main metabolite content and radical scavenging activity. Penerbit Universiti Kebangsaan Malaysia 2022-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/19752/1/15.pdf Rafi, Mohamad and Anastasia Grandivoriana Nomi, and Dewi Anggraini Septaningsih, and Heryanto, Rudi and Sastia Prama Putri, (2022) Quantitative HPLC and FTIR-based metabolomics for clustering Centella asiatica cultivation ages and evaluation of their radical scavenging activity. Sains Malaysiana, 51 (6). pp. 1789-1797. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil6_2022/KandunganJilid51Bil6_2022.html |
| spellingShingle | Rafi, Mohamad Anastasia Grandivoriana Nomi, Dewi Anggraini Septaningsih, Heryanto, Rudi Sastia Prama Putri, Quantitative HPLC and FTIR-based metabolomics for clustering Centella asiatica cultivation ages and evaluation of their radical scavenging activity |
| title | Quantitative HPLC and FTIR-based metabolomics for clustering Centella asiatica cultivation ages and evaluation of their radical scavenging activity |
| title_full | Quantitative HPLC and FTIR-based metabolomics for clustering Centella asiatica cultivation ages and evaluation of their radical scavenging activity |
| title_fullStr | Quantitative HPLC and FTIR-based metabolomics for clustering Centella asiatica cultivation ages and evaluation of their radical scavenging activity |
| title_full_unstemmed | Quantitative HPLC and FTIR-based metabolomics for clustering Centella asiatica cultivation ages and evaluation of their radical scavenging activity |
| title_short | Quantitative HPLC and FTIR-based metabolomics for clustering Centella asiatica cultivation ages and evaluation of their radical scavenging activity |
| title_sort | quantitative hplc and ftir-based metabolomics for clustering centella asiatica cultivation ages and evaluation of their radical scavenging activity |
| url | http://journalarticle.ukm.my/19752/ http://journalarticle.ukm.my/19752/ http://journalarticle.ukm.my/19752/1/15.pdf |