Diversity and antibacterial and antioxidant activities of fungal endophytes from the roots of Eucalyptus deglupta
In this study, 45 endophytic fungal strains were isolated from the roots of Eucalyptus deglupta. Among them, 16 distinct strains were identified and classified into 14 different genera (Celoporthe, Aspergillus, Castanediella, Chaetomium, Biscogniauxia, Sordariales, Pestalotiopsis, Clitopilus, Cylind...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/19744/ http://journalarticle.ukm.my/19744/1/7.pdf |
| _version_ | 1848814920164966400 |
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| author | Wang, Wei Mao, Ziling Wu, Chunyin Hamza Shahid, Wang, Shengkun Shan, Tijiang |
| author_facet | Wang, Wei Mao, Ziling Wu, Chunyin Hamza Shahid, Wang, Shengkun Shan, Tijiang |
| author_sort | Wang, Wei |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | In this study, 45 endophytic fungal strains were isolated from the roots of Eucalyptus deglupta. Among them, 16 distinct strains were identified and classified into 14 different genera (Celoporthe, Aspergillus, Castanediella, Chaetomium, Biscogniauxia, Sordariales, Pestalotiopsis, Clitopilus, Cylindrocladiella, Calonectria, Trichoderma, Xylaria, Neofusicoccum and Pleosporales) according to their morphological characteristics and molecular information. The genera Aspergillus and Calonectria were the dominant endophytic fungi in the roots of E. deglupta. In addition, the antibacterial and antioxidant activities of the 16 endophytic fungi isolated from the roots of E. deglupta were evaluated. All the strains displayed inhibitory activities against Agrobacterium tumefaciens, Bacillus subtilis, Escherichia coli, and Xanthomonas vesicatoria. Strains Edf-1 to Edf-4, Edf-11 and Edf-12 demonstrated strong inhibitory activity against R. solanacearum with plaque diameters between 5 and 10 mm. The crude extract of Edf-14 had inhibitory activity against all tested bacteria. Five strains, Edf-1 to Edf-5, demonstrated a strong scavenging capacity for 2,2-diphenyl-1-picrylhydrazyl (DPPH), with IC50 values of 0.26 ± 0.04, 0.11 ± 0.03, 0.20 ± 0.05, 0.10 ± 0.04 and 0.14 ± 0.02 mg/mL, respectively. Hence, endophytic fungi isolated from the roots of E. deglupta showed antibacterial and antioxidant activities, providing a theoretical foundation for further isolation and identification of specific active components. |
| first_indexed | 2025-11-15T00:41:45Z |
| format | Article |
| id | oai:generic.eprints.org:19744 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:41:45Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:197442022-09-19T06:45:27Z http://journalarticle.ukm.my/19744/ Diversity and antibacterial and antioxidant activities of fungal endophytes from the roots of Eucalyptus deglupta Wang, Wei Mao, Ziling Wu, Chunyin Hamza Shahid, Wang, Shengkun Shan, Tijiang In this study, 45 endophytic fungal strains were isolated from the roots of Eucalyptus deglupta. Among them, 16 distinct strains were identified and classified into 14 different genera (Celoporthe, Aspergillus, Castanediella, Chaetomium, Biscogniauxia, Sordariales, Pestalotiopsis, Clitopilus, Cylindrocladiella, Calonectria, Trichoderma, Xylaria, Neofusicoccum and Pleosporales) according to their morphological characteristics and molecular information. The genera Aspergillus and Calonectria were the dominant endophytic fungi in the roots of E. deglupta. In addition, the antibacterial and antioxidant activities of the 16 endophytic fungi isolated from the roots of E. deglupta were evaluated. All the strains displayed inhibitory activities against Agrobacterium tumefaciens, Bacillus subtilis, Escherichia coli, and Xanthomonas vesicatoria. Strains Edf-1 to Edf-4, Edf-11 and Edf-12 demonstrated strong inhibitory activity against R. solanacearum with plaque diameters between 5 and 10 mm. The crude extract of Edf-14 had inhibitory activity against all tested bacteria. Five strains, Edf-1 to Edf-5, demonstrated a strong scavenging capacity for 2,2-diphenyl-1-picrylhydrazyl (DPPH), with IC50 values of 0.26 ± 0.04, 0.11 ± 0.03, 0.20 ± 0.05, 0.10 ± 0.04 and 0.14 ± 0.02 mg/mL, respectively. Hence, endophytic fungi isolated from the roots of E. deglupta showed antibacterial and antioxidant activities, providing a theoretical foundation for further isolation and identification of specific active components. Penerbit Universiti Kebangsaan Malaysia 2022-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/19744/1/7.pdf Wang, Wei and Mao, Ziling and Wu, Chunyin and Hamza Shahid, and Wang, Shengkun and Shan, Tijiang (2022) Diversity and antibacterial and antioxidant activities of fungal endophytes from the roots of Eucalyptus deglupta. Sains Malaysiana, 51 (6). pp. 1687-1696. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil6_2022/KandunganJilid51Bil6_2022.html |
| spellingShingle | Wang, Wei Mao, Ziling Wu, Chunyin Hamza Shahid, Wang, Shengkun Shan, Tijiang Diversity and antibacterial and antioxidant activities of fungal endophytes from the roots of Eucalyptus deglupta |
| title | Diversity and antibacterial and antioxidant activities of fungal endophytes from the roots of Eucalyptus deglupta |
| title_full | Diversity and antibacterial and antioxidant activities of fungal endophytes from the roots of Eucalyptus deglupta |
| title_fullStr | Diversity and antibacterial and antioxidant activities of fungal endophytes from the roots of Eucalyptus deglupta |
| title_full_unstemmed | Diversity and antibacterial and antioxidant activities of fungal endophytes from the roots of Eucalyptus deglupta |
| title_short | Diversity and antibacterial and antioxidant activities of fungal endophytes from the roots of Eucalyptus deglupta |
| title_sort | diversity and antibacterial and antioxidant activities of fungal endophytes from the roots of eucalyptus deglupta |
| url | http://journalarticle.ukm.my/19744/ http://journalarticle.ukm.my/19744/ http://journalarticle.ukm.my/19744/1/7.pdf |