Tualang honey promotes apoptosis of the A549 lung adenocarcinoma cell line via modulation of PI3K/AKT signaling pathway-related proteins
The phosphatidylinositol 3-kinases (PI3Ks)/protein kinase B (AKT) signaling pathway is frequently overexpressed in lung adenocarcinoma and associated with carcinogenesis through cell proliferation, and apoptosis deactivation; and it also enhances chemotherapeutic drugs resistance. Tualang honey (...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pusat Perubatan Universiti Kebangsaan Malaysia
2022
|
| Online Access: | http://journalarticle.ukm.my/19672/ http://journalarticle.ukm.my/19672/1/16_ms0561_pdf_50460.pdf |
| _version_ | 1848814901750923264 |
|---|---|
| author | Nazirah A, Puteri Shafinaz Abdul-Rahman, |
| author_facet | Nazirah A, Puteri Shafinaz Abdul-Rahman, |
| author_sort | Nazirah A, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The phosphatidylinositol 3-kinases (PI3Ks)/protein kinase B (AKT) signaling
pathway is frequently overexpressed in lung adenocarcinoma and associated
with carcinogenesis through cell proliferation, and apoptosis deactivation; and
it also enhances chemotherapeutic drugs resistance. Tualang honey (TH) has
proven anticancer property on lung adenocarcinoma. However, the underlying
mechanisms remain unreported. Hence, this study investigates the apoptosisinducing
effect of TH on A549 lung adenocarcinoma cell line using RayBio® Human
Apoptosis Array and label-free quantitative liquid chromatography-tandem mass
spectrometry (LC-MS/MS) analysis to elucidate the modulation of upstream and
downstream proteins of PI3K/AKT signaling pathway. The apoptosis-promoting
effects of TH were associated with (i) the upregulation of pro-apoptotic proteins (i.e.
cytochrome c, histone H1.2, and histone H1.4) and tumor suppressor proteins (i.e.
IGFBP-3 and IGFBP-5), and (ii) downregulation of XIAP, insulin-growth factor (IGF)
system (i.e. IGF-1, IGF1R, IGFBP-1, IGFBP-2, and IGFBP-4), HSP90AB1, YWHAQ,
endoplasmin, and ITGB1. The effects were also linked with the suppression of
receptor tyrosine kinase, integrins, G proteins, CHUK, RAC1, and JAK. Thus, TH
may promote apoptosis of A549 lung adenocarcinoma cell line through alteration
of the PI3K/AKT signaling pathway-related proteins. However, further studies
involving animal models to provide a better model of understanding would prove
necessary. |
| first_indexed | 2025-11-15T00:41:27Z |
| format | Article |
| id | oai:generic.eprints.org:19672 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:41:27Z |
| publishDate | 2022 |
| publisher | Pusat Perubatan Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:196722022-09-12T02:05:01Z http://journalarticle.ukm.my/19672/ Tualang honey promotes apoptosis of the A549 lung adenocarcinoma cell line via modulation of PI3K/AKT signaling pathway-related proteins Nazirah A, Puteri Shafinaz Abdul-Rahman, The phosphatidylinositol 3-kinases (PI3Ks)/protein kinase B (AKT) signaling pathway is frequently overexpressed in lung adenocarcinoma and associated with carcinogenesis through cell proliferation, and apoptosis deactivation; and it also enhances chemotherapeutic drugs resistance. Tualang honey (TH) has proven anticancer property on lung adenocarcinoma. However, the underlying mechanisms remain unreported. Hence, this study investigates the apoptosisinducing effect of TH on A549 lung adenocarcinoma cell line using RayBio® Human Apoptosis Array and label-free quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to elucidate the modulation of upstream and downstream proteins of PI3K/AKT signaling pathway. The apoptosis-promoting effects of TH were associated with (i) the upregulation of pro-apoptotic proteins (i.e. cytochrome c, histone H1.2, and histone H1.4) and tumor suppressor proteins (i.e. IGFBP-3 and IGFBP-5), and (ii) downregulation of XIAP, insulin-growth factor (IGF) system (i.e. IGF-1, IGF1R, IGFBP-1, IGFBP-2, and IGFBP-4), HSP90AB1, YWHAQ, endoplasmin, and ITGB1. The effects were also linked with the suppression of receptor tyrosine kinase, integrins, G proteins, CHUK, RAC1, and JAK. Thus, TH may promote apoptosis of A549 lung adenocarcinoma cell line through alteration of the PI3K/AKT signaling pathway-related proteins. However, further studies involving animal models to provide a better model of understanding would prove necessary. Pusat Perubatan Universiti Kebangsaan Malaysia 2022-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/19672/1/16_ms0561_pdf_50460.pdf Nazirah A, and Puteri Shafinaz Abdul-Rahman, (2022) Tualang honey promotes apoptosis of the A549 lung adenocarcinoma cell line via modulation of PI3K/AKT signaling pathway-related proteins. Medicine & Health, 17 (1). pp. 211-225. ISSN 2289-5728 https://www.medicineandhealthukm.com/toc/17/1 |
| spellingShingle | Nazirah A, Puteri Shafinaz Abdul-Rahman, Tualang honey promotes apoptosis of the A549 lung adenocarcinoma cell line via modulation of PI3K/AKT signaling pathway-related proteins |
| title | Tualang honey promotes apoptosis of the A549 lung adenocarcinoma cell line via modulation of PI3K/AKT signaling pathway-related proteins |
| title_full | Tualang honey promotes apoptosis of the A549 lung adenocarcinoma cell line via modulation of PI3K/AKT signaling pathway-related proteins |
| title_fullStr | Tualang honey promotes apoptosis of the A549 lung adenocarcinoma cell line via modulation of PI3K/AKT signaling pathway-related proteins |
| title_full_unstemmed | Tualang honey promotes apoptosis of the A549 lung adenocarcinoma cell line via modulation of PI3K/AKT signaling pathway-related proteins |
| title_short | Tualang honey promotes apoptosis of the A549 lung adenocarcinoma cell line via modulation of PI3K/AKT signaling pathway-related proteins |
| title_sort | tualang honey promotes apoptosis of the a549 lung adenocarcinoma cell line via modulation of pi3k/akt signaling pathway-related proteins |
| url | http://journalarticle.ukm.my/19672/ http://journalarticle.ukm.my/19672/ http://journalarticle.ukm.my/19672/1/16_ms0561_pdf_50460.pdf |