Tualang honey promotes apoptosis of the A549 lung adenocarcinoma cell line via modulation of PI3K/AKT signaling pathway-related proteins
The phosphatidylinositol 3-kinases (PI3Ks)/protein kinase B (AKT) signaling pathway is frequently overexpressed in lung adenocarcinoma and associated with carcinogenesis through cell proliferation, and apoptosis deactivation; and it also enhances chemotherapeutic drugs resistance. Tualang honey (...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Pusat Perubatan Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/19672/ http://journalarticle.ukm.my/19672/1/16_ms0561_pdf_50460.pdf |
| Summary: | The phosphatidylinositol 3-kinases (PI3Ks)/protein kinase B (AKT) signaling
pathway is frequently overexpressed in lung adenocarcinoma and associated
with carcinogenesis through cell proliferation, and apoptosis deactivation; and
it also enhances chemotherapeutic drugs resistance. Tualang honey (TH) has
proven anticancer property on lung adenocarcinoma. However, the underlying
mechanisms remain unreported. Hence, this study investigates the apoptosisinducing
effect of TH on A549 lung adenocarcinoma cell line using RayBio® Human
Apoptosis Array and label-free quantitative liquid chromatography-tandem mass
spectrometry (LC-MS/MS) analysis to elucidate the modulation of upstream and
downstream proteins of PI3K/AKT signaling pathway. The apoptosis-promoting
effects of TH were associated with (i) the upregulation of pro-apoptotic proteins (i.e.
cytochrome c, histone H1.2, and histone H1.4) and tumor suppressor proteins (i.e.
IGFBP-3 and IGFBP-5), and (ii) downregulation of XIAP, insulin-growth factor (IGF)
system (i.e. IGF-1, IGF1R, IGFBP-1, IGFBP-2, and IGFBP-4), HSP90AB1, YWHAQ,
endoplasmin, and ITGB1. The effects were also linked with the suppression of
receptor tyrosine kinase, integrins, G proteins, CHUK, RAC1, and JAK. Thus, TH
may promote apoptosis of A549 lung adenocarcinoma cell line through alteration
of the PI3K/AKT signaling pathway-related proteins. However, further studies
involving animal models to provide a better model of understanding would prove
necessary. |
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