Optimization of ultrasound-assisted extraction of total flavonoids content from the white flowering variety of Melastoma Malabathricum

Melastoma malabathricum or Senduduk Putih is one of the herbs listed under National Key Economic Area (NKEA) of Malaysia. It has the potential to be used as remedies that is rich in natural flavonoids. One of the innovations for extraction in modern technology is Ultrasound-Assisted Extraction (UA...

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Main Authors: Lee, Chia Hau, Lee, Ting Hun, Harisun Ya’akob, Wong, Syieluing, Jannet, Hichem Ben
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/19601/
http://journalarticle.ukm.my/19601/1/12.pdf
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author Lee, Chia Hau
Lee, Ting Hun
Harisun Ya’akob,
Wong, Syieluing
Jannet, Hichem Ben
author_facet Lee, Chia Hau
Lee, Ting Hun
Harisun Ya’akob,
Wong, Syieluing
Jannet, Hichem Ben
author_sort Lee, Chia Hau
building UKM Institutional Repository
collection Online Access
description Melastoma malabathricum or Senduduk Putih is one of the herbs listed under National Key Economic Area (NKEA) of Malaysia. It has the potential to be used as remedies that is rich in natural flavonoids. One of the innovations for extraction in modern technology is Ultrasound-Assisted Extraction (UAE) that showed several advantages. Therefore, this study aimed to explore using UAE to extract the total flavonoids in M. malabathricum. After that, UAE is optimized coupled with a stirrer for total flavonoid extraction. Five operating parameters: ethanol concentration, plant material to solvent ratio, extraction temperature, extraction time and ultrasound power were investigated by one-factor-at-a-time (OFAT) method to choose the most important parameters. After that, the response surface methodology coupled with face-centered central composite design (FCCD) was employed to study the interaction of the three keys parameters. The optimized conditions to extract total flavonoids content by UAE with constant mixing speed (300 ± 6 rpm) were at a ratio of 1g of plant material: 30 mL of 100% (v/v) ethanol, extraction temperature at 70oC, 19 min of extraction time and 280 W of ultrasound power. The experimental yields of total flavonoids content were 64.94 mg/g, which is well matched with the predicted value of 63.75 mg/g.
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spelling oai:generic.eprints.org:196012022-09-08T02:56:15Z http://journalarticle.ukm.my/19601/ Optimization of ultrasound-assisted extraction of total flavonoids content from the white flowering variety of Melastoma Malabathricum Lee, Chia Hau Lee, Ting Hun Harisun Ya’akob, Wong, Syieluing Jannet, Hichem Ben Melastoma malabathricum or Senduduk Putih is one of the herbs listed under National Key Economic Area (NKEA) of Malaysia. It has the potential to be used as remedies that is rich in natural flavonoids. One of the innovations for extraction in modern technology is Ultrasound-Assisted Extraction (UAE) that showed several advantages. Therefore, this study aimed to explore using UAE to extract the total flavonoids in M. malabathricum. After that, UAE is optimized coupled with a stirrer for total flavonoid extraction. Five operating parameters: ethanol concentration, plant material to solvent ratio, extraction temperature, extraction time and ultrasound power were investigated by one-factor-at-a-time (OFAT) method to choose the most important parameters. After that, the response surface methodology coupled with face-centered central composite design (FCCD) was employed to study the interaction of the three keys parameters. The optimized conditions to extract total flavonoids content by UAE with constant mixing speed (300 ± 6 rpm) were at a ratio of 1g of plant material: 30 mL of 100% (v/v) ethanol, extraction temperature at 70oC, 19 min of extraction time and 280 W of ultrasound power. The experimental yields of total flavonoids content were 64.94 mg/g, which is well matched with the predicted value of 63.75 mg/g. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/19601/1/12.pdf Lee, Chia Hau and Lee, Ting Hun and Harisun Ya’akob, and Wong, Syieluing and Jannet, Hichem Ben (2019) Optimization of ultrasound-assisted extraction of total flavonoids content from the white flowering variety of Melastoma Malabathricum. Jurnal Kejuruteraan, 2 (1(SI)). pp. 91-102. ISSN 0128-0198 https://www.ukm.my/jkukm/si-21-2019/
spellingShingle Lee, Chia Hau
Lee, Ting Hun
Harisun Ya’akob,
Wong, Syieluing
Jannet, Hichem Ben
Optimization of ultrasound-assisted extraction of total flavonoids content from the white flowering variety of Melastoma Malabathricum
title Optimization of ultrasound-assisted extraction of total flavonoids content from the white flowering variety of Melastoma Malabathricum
title_full Optimization of ultrasound-assisted extraction of total flavonoids content from the white flowering variety of Melastoma Malabathricum
title_fullStr Optimization of ultrasound-assisted extraction of total flavonoids content from the white flowering variety of Melastoma Malabathricum
title_full_unstemmed Optimization of ultrasound-assisted extraction of total flavonoids content from the white flowering variety of Melastoma Malabathricum
title_short Optimization of ultrasound-assisted extraction of total flavonoids content from the white flowering variety of Melastoma Malabathricum
title_sort optimization of ultrasound-assisted extraction of total flavonoids content from the white flowering variety of melastoma malabathricum
url http://journalarticle.ukm.my/19601/
http://journalarticle.ukm.my/19601/
http://journalarticle.ukm.my/19601/1/12.pdf