Molecular pcr assays for detection of Ganoderma pathogenic to oil palm in Malaysia
Ganoderma boninense is a fungal pathogen that causes basal stem rot (BSR) disease in oil palm. Being a serious disease problem to the oil palm industry, monitoring and detecting the pathogen is of the utmost importance to reduce disease spread and facilitate effective management strategies. Becaus...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/19522/ http://journalarticle.ukm.my/19522/1/17%2B-%2BMOLECULAR%2BPCR%2BASSAYS%2BFOR%2BDETECTION%2BOF%2BGanoderma.pdf |
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| author | Nur Hailini Zainol Hilmi, Abu Seman Idris, Mohamed Maizatul-Suriza, Ahmad Zairun Madihah, Ramli Nur-Rashyeda, |
| author_facet | Nur Hailini Zainol Hilmi, Abu Seman Idris, Mohamed Maizatul-Suriza, Ahmad Zairun Madihah, Ramli Nur-Rashyeda, |
| author_sort | Nur Hailini Zainol Hilmi, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Ganoderma boninense is a fungal pathogen that causes basal stem rot (BSR) disease in oil palm. Being a serious disease
problem to the oil palm industry, monitoring and detecting the pathogen is of the utmost importance to reduce disease spread
and facilitate effective management strategies. Because the traditional culture-based assay is time-consuming, labor-intensive,
and required special skills in mycology, plant pathologists are turning to more accurate, sensitive, and fast methods such as
molecular techniques. In this study, polymerase chain reaction (PCR) assays were developed to detect pathogenic Ganoderma
species causing BSR disease in oil palm using a primer designed based on the ribosomal DNA internal transcribed spacer (ITS)
region. The effectiveness of conventional and real-time PCR assays was analyzed compared to the traditional isolation-based
assay. For artificially inoculated oil palm plantlets, consistent detection of G. boninense was observed. Real-time PCR assay has
shown to be more sensitive and rapid in detecting G. boninense in field samples and could potentially serve as a validation tool
to other detection techniques for the implementation of effective disease control measures. |
| first_indexed | 2025-11-15T00:40:51Z |
| format | Article |
| id | oai:generic.eprints.org:19522 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:40:51Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:195222022-08-30T06:05:06Z http://journalarticle.ukm.my/19522/ Molecular pcr assays for detection of Ganoderma pathogenic to oil palm in Malaysia Nur Hailini Zainol Hilmi, Abu Seman Idris, Mohamed Maizatul-Suriza, Ahmad Zairun Madihah, Ramli Nur-Rashyeda, Ganoderma boninense is a fungal pathogen that causes basal stem rot (BSR) disease in oil palm. Being a serious disease problem to the oil palm industry, monitoring and detecting the pathogen is of the utmost importance to reduce disease spread and facilitate effective management strategies. Because the traditional culture-based assay is time-consuming, labor-intensive, and required special skills in mycology, plant pathologists are turning to more accurate, sensitive, and fast methods such as molecular techniques. In this study, polymerase chain reaction (PCR) assays were developed to detect pathogenic Ganoderma species causing BSR disease in oil palm using a primer designed based on the ribosomal DNA internal transcribed spacer (ITS) region. The effectiveness of conventional and real-time PCR assays was analyzed compared to the traditional isolation-based assay. For artificially inoculated oil palm plantlets, consistent detection of G. boninense was observed. Real-time PCR assay has shown to be more sensitive and rapid in detecting G. boninense in field samples and could potentially serve as a validation tool to other detection techniques for the implementation of effective disease control measures. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/19522/1/17%2B-%2BMOLECULAR%2BPCR%2BASSAYS%2BFOR%2BDETECTION%2BOF%2BGanoderma.pdf Nur Hailini Zainol Hilmi, and Abu Seman Idris, and Mohamed Maizatul-Suriza, and Ahmad Zairun Madihah, and Ramli Nur-Rashyeda, (2022) Molecular pcr assays for detection of Ganoderma pathogenic to oil palm in Malaysia. Malaysian Applied Biology, 51 (1). pp. 171-181. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/44 |
| spellingShingle | Nur Hailini Zainol Hilmi, Abu Seman Idris, Mohamed Maizatul-Suriza, Ahmad Zairun Madihah, Ramli Nur-Rashyeda, Molecular pcr assays for detection of Ganoderma pathogenic to oil palm in Malaysia |
| title | Molecular pcr assays for detection of Ganoderma pathogenic to oil palm in Malaysia |
| title_full | Molecular pcr assays for detection of Ganoderma pathogenic to oil palm in Malaysia |
| title_fullStr | Molecular pcr assays for detection of Ganoderma pathogenic to oil palm in Malaysia |
| title_full_unstemmed | Molecular pcr assays for detection of Ganoderma pathogenic to oil palm in Malaysia |
| title_short | Molecular pcr assays for detection of Ganoderma pathogenic to oil palm in Malaysia |
| title_sort | molecular pcr assays for detection of ganoderma pathogenic to oil palm in malaysia |
| url | http://journalarticle.ukm.my/19522/ http://journalarticle.ukm.my/19522/ http://journalarticle.ukm.my/19522/1/17%2B-%2BMOLECULAR%2BPCR%2BASSAYS%2BFOR%2BDETECTION%2BOF%2BGanoderma.pdf |