Antioxidant capabilities of Litsea garciae bark extracts and their relation to the phytochemical compositions

The plant species belonging to the Litsea genus are widely investigated due to their nutritional and medicinal purposes. In this regard, this study is another similar sincere effort in which the antioxidant property and phytochemical composition of Litsea garciae (L. garciae) bark’s hexane, chloro...

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Main Authors: Siti Zaleha Raduan, Qamar Uddin Ahmed, Abdul Razak Kasmuri, Muhamad Rusdi Ahmad Rusmili, Md Abdur Rashid Mia, Wan Mohd Azizi Wan Sulaiman, Muhammad Hamdi Mahmood, Mohd Farooq Shaikh
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2022
Online Access:http://journalarticle.ukm.my/19515/
http://journalarticle.ukm.my/19515/1/10%2B-%2BANTIOXIDANT%2BCAPABILITIES%2BOF%2BLitsea%2Bgarciae%2BBARK%2BEXTRACTS%2B%281%29.pdf
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author Siti Zaleha Raduan,
Qamar Uddin Ahmed,
Abdul Razak Kasmuri,
Muhamad Rusdi Ahmad Rusmili,
Md Abdur Rashid Mia,
Wan Mohd Azizi Wan Sulaiman,
Muhammad Hamdi Mahmood,
Mohd Farooq Shaikh,
author_facet Siti Zaleha Raduan,
Qamar Uddin Ahmed,
Abdul Razak Kasmuri,
Muhamad Rusdi Ahmad Rusmili,
Md Abdur Rashid Mia,
Wan Mohd Azizi Wan Sulaiman,
Muhammad Hamdi Mahmood,
Mohd Farooq Shaikh,
author_sort Siti Zaleha Raduan,
building UKM Institutional Repository
collection Online Access
description The plant species belonging to the Litsea genus are widely investigated due to their nutritional and medicinal purposes. In this regard, this study is another similar sincere effort in which the antioxidant property and phytochemical composition of Litsea garciae (L. garciae) bark’s hexane, chloroform, methanol, and aqueous extracts were evaluated to confirm its traditional benefits. The total flavonoid content (TFC) and total phenolic content (TPC) were determined first, followed by an assessment of in vitro antioxidant activity using the DPPH and FRAP assays. The composition of the secondary metabolites was determined using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS). As a result, methanol extract was recorded to have the highest TPC value aligned with its positive appearance in phytochemical screening. Its antioxidant capacity indicated the least IC50. The results indicated that the significant free radical scavenging activity was due to the methanolic extract’s high phenolic content. The secondary metabolites found in the methanol extract varied significantly according to UHPLC-MS analysis. The major phenolic compounds were found including N-trans-feruloyl-4-O-methyldopamine, N-cisferuloyltyramine, epicatechin-(4beta->6)-epicatechin-(2beta->7,4beta->8)-epicatechin, 7-Hydroxy-3-(4-methoxyphenyl)- 4-propyl-2H-1-benzopyran-2-one and 9-O-Methylneodunol. In general, the results indicate that L. garciae bark may be a promising source of novel natural compounds with antioxidative properties.
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spelling oai:generic.eprints.org:195152022-08-30T00:58:24Z http://journalarticle.ukm.my/19515/ Antioxidant capabilities of Litsea garciae bark extracts and their relation to the phytochemical compositions Siti Zaleha Raduan, Qamar Uddin Ahmed, Abdul Razak Kasmuri, Muhamad Rusdi Ahmad Rusmili, Md Abdur Rashid Mia, Wan Mohd Azizi Wan Sulaiman, Muhammad Hamdi Mahmood, Mohd Farooq Shaikh, The plant species belonging to the Litsea genus are widely investigated due to their nutritional and medicinal purposes. In this regard, this study is another similar sincere effort in which the antioxidant property and phytochemical composition of Litsea garciae (L. garciae) bark’s hexane, chloroform, methanol, and aqueous extracts were evaluated to confirm its traditional benefits. The total flavonoid content (TFC) and total phenolic content (TPC) were determined first, followed by an assessment of in vitro antioxidant activity using the DPPH and FRAP assays. The composition of the secondary metabolites was determined using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS). As a result, methanol extract was recorded to have the highest TPC value aligned with its positive appearance in phytochemical screening. Its antioxidant capacity indicated the least IC50. The results indicated that the significant free radical scavenging activity was due to the methanolic extract’s high phenolic content. The secondary metabolites found in the methanol extract varied significantly according to UHPLC-MS analysis. The major phenolic compounds were found including N-trans-feruloyl-4-O-methyldopamine, N-cisferuloyltyramine, epicatechin-(4beta->6)-epicatechin-(2beta->7,4beta->8)-epicatechin, 7-Hydroxy-3-(4-methoxyphenyl)- 4-propyl-2H-1-benzopyran-2-one and 9-O-Methylneodunol. In general, the results indicate that L. garciae bark may be a promising source of novel natural compounds with antioxidative properties. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/19515/1/10%2B-%2BANTIOXIDANT%2BCAPABILITIES%2BOF%2BLitsea%2Bgarciae%2BBARK%2BEXTRACTS%2B%281%29.pdf Siti Zaleha Raduan, and Qamar Uddin Ahmed, and Abdul Razak Kasmuri, and Muhamad Rusdi Ahmad Rusmili, and Md Abdur Rashid Mia, and Wan Mohd Azizi Wan Sulaiman, and Muhammad Hamdi Mahmood, and Mohd Farooq Shaikh, (2022) Antioxidant capabilities of Litsea garciae bark extracts and their relation to the phytochemical compositions. Malaysian Applied Biology, 51 (1). pp. 99-117. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/44
spellingShingle Siti Zaleha Raduan,
Qamar Uddin Ahmed,
Abdul Razak Kasmuri,
Muhamad Rusdi Ahmad Rusmili,
Md Abdur Rashid Mia,
Wan Mohd Azizi Wan Sulaiman,
Muhammad Hamdi Mahmood,
Mohd Farooq Shaikh,
Antioxidant capabilities of Litsea garciae bark extracts and their relation to the phytochemical compositions
title Antioxidant capabilities of Litsea garciae bark extracts and their relation to the phytochemical compositions
title_full Antioxidant capabilities of Litsea garciae bark extracts and their relation to the phytochemical compositions
title_fullStr Antioxidant capabilities of Litsea garciae bark extracts and their relation to the phytochemical compositions
title_full_unstemmed Antioxidant capabilities of Litsea garciae bark extracts and their relation to the phytochemical compositions
title_short Antioxidant capabilities of Litsea garciae bark extracts and their relation to the phytochemical compositions
title_sort antioxidant capabilities of litsea garciae bark extracts and their relation to the phytochemical compositions
url http://journalarticle.ukm.my/19515/
http://journalarticle.ukm.my/19515/
http://journalarticle.ukm.my/19515/1/10%2B-%2BANTIOXIDANT%2BCAPABILITIES%2BOF%2BLitsea%2Bgarciae%2BBARK%2BEXTRACTS%2B%281%29.pdf