Formulation of entomopathogen Metarhizium anisopliae var. anisopliae bait to control subterranean termite, Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)

Coptotermes curvignathus is well known as the leading pest termite of agricultural and forestry tree crops. Termites are cryptic insects, hence their infestations can go undetected for years and this hinders the planters to carry out effective pest controls timely. This underscores the need for i...

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Main Authors: Nyam, Vei Ting, Bong, Joseph Choon Fah, Ong, Kian Huat, King, Patricia Jie Hung
Format: Article
Language:English
Published: Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2021
Online Access:http://journalarticle.ukm.my/18981/
http://journalarticle.ukm.my/18981/1/47638-173413-1-PB.pdf
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author Nyam, Vei Ting
Bong, Joseph Choon Fah
Ong, Kian Huat
King, Patricia Jie Hung
author_facet Nyam, Vei Ting
Bong, Joseph Choon Fah
Ong, Kian Huat
King, Patricia Jie Hung
author_sort Nyam, Vei Ting
building UKM Institutional Repository
collection Online Access
description Coptotermes curvignathus is well known as the leading pest termite of agricultural and forestry tree crops. Termites are cryptic insects, hence their infestations can go undetected for years and this hinders the planters to carry out effective pest controls timely. This underscores the need for innovative forms of pesticides deployment. The use of bait is becoming a favorable approach that acts as edible ‘smart missiles’ to knock out the whole population of termites. To execute potent baiting in the field, effective bait matrix that able to preserve the effectiveness of the entomopathogenic agents impregnated and avoid the repellence of the targeted insect is sought-after in designing baits and this study aimed to formulate an entomopathogenic fungus Metarhizium anisopliae var. anisopliae based bait. Seven baits were formulated based on the pest feeding preferences and termites’ behavior studies. Additional substances were supplemented in the formulas as food sources and attractant to termites. Bait matrix made of dextrin, PVP K90 and skim milk (DPS) was found to be the most palatable to termites than other bait matrix tested. Termite took shorter time to finish the DPS bait with average 11.2 days and consumed significantly greater amount as compared to the other baits. DPS bait impregnated with M. anisopliae resulted closed to 100% mortality in 19 days. Based on shelflife bioassays, all formulated baits were able to maintain consistent termite mortality above 80% for over six months. The laboratory assessment had concluded that the formulated DPS bait was able to retain the effectiveness of the active entomopathogenic ingredient and appear palatable to C. curvignathus. Future field work should concentrate on the effectiveness of this bait in deterring pest resurgence.
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spelling oai:generic.eprints.org:189812022-07-20T06:16:05Z http://journalarticle.ukm.my/18981/ Formulation of entomopathogen Metarhizium anisopliae var. anisopliae bait to control subterranean termite, Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae) Nyam, Vei Ting Bong, Joseph Choon Fah Ong, Kian Huat King, Patricia Jie Hung Coptotermes curvignathus is well known as the leading pest termite of agricultural and forestry tree crops. Termites are cryptic insects, hence their infestations can go undetected for years and this hinders the planters to carry out effective pest controls timely. This underscores the need for innovative forms of pesticides deployment. The use of bait is becoming a favorable approach that acts as edible ‘smart missiles’ to knock out the whole population of termites. To execute potent baiting in the field, effective bait matrix that able to preserve the effectiveness of the entomopathogenic agents impregnated and avoid the repellence of the targeted insect is sought-after in designing baits and this study aimed to formulate an entomopathogenic fungus Metarhizium anisopliae var. anisopliae based bait. Seven baits were formulated based on the pest feeding preferences and termites’ behavior studies. Additional substances were supplemented in the formulas as food sources and attractant to termites. Bait matrix made of dextrin, PVP K90 and skim milk (DPS) was found to be the most palatable to termites than other bait matrix tested. Termite took shorter time to finish the DPS bait with average 11.2 days and consumed significantly greater amount as compared to the other baits. DPS bait impregnated with M. anisopliae resulted closed to 100% mortality in 19 days. Based on shelflife bioassays, all formulated baits were able to maintain consistent termite mortality above 80% for over six months. The laboratory assessment had concluded that the formulated DPS bait was able to retain the effectiveness of the active entomopathogenic ingredient and appear palatable to C. curvignathus. Future field work should concentrate on the effectiveness of this bait in deterring pest resurgence. Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2021 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18981/1/47638-173413-1-PB.pdf Nyam, Vei Ting and Bong, Joseph Choon Fah and Ong, Kian Huat and King, Patricia Jie Hung (2021) Formulation of entomopathogen Metarhizium anisopliae var. anisopliae bait to control subterranean termite, Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae). Serangga, 26 (4). pp. 42-59. ISSN 1394-5130 https://ejournal.ukm.my/serangga/issue/view/1448/showToc
spellingShingle Nyam, Vei Ting
Bong, Joseph Choon Fah
Ong, Kian Huat
King, Patricia Jie Hung
Formulation of entomopathogen Metarhizium anisopliae var. anisopliae bait to control subterranean termite, Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title Formulation of entomopathogen Metarhizium anisopliae var. anisopliae bait to control subterranean termite, Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title_full Formulation of entomopathogen Metarhizium anisopliae var. anisopliae bait to control subterranean termite, Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title_fullStr Formulation of entomopathogen Metarhizium anisopliae var. anisopliae bait to control subterranean termite, Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title_full_unstemmed Formulation of entomopathogen Metarhizium anisopliae var. anisopliae bait to control subterranean termite, Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title_short Formulation of entomopathogen Metarhizium anisopliae var. anisopliae bait to control subterranean termite, Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title_sort formulation of entomopathogen metarhizium anisopliae var. anisopliae bait to control subterranean termite, coptotermes curvignathus holmgren (blattodea: rhinotermitidae)
url http://journalarticle.ukm.my/18981/
http://journalarticle.ukm.my/18981/
http://journalarticle.ukm.my/18981/1/47638-173413-1-PB.pdf