Efficacy of live attenuated fowl adenovirus serotype 8B isolate of Malaysia in specific pathogen-free chickens

Fowl adenovirus (FAdV) is the primary agent of inclusion body hepatitis (IBH) in poultry and caused serious economic impact due to high mortality and poor productivity. To date, clinical cases of IBH in chickens increases over the years in Malaysia, thus, an effective local vaccine to control the...

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Main Authors: Norfitriah Mohamed Sohaimi, Mohd Hair Bejo
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Online Access:http://journalarticle.ukm.my/18892/
http://journalarticle.ukm.my/18892/1/135-143%2B%2B%2BNorfitriah%2BMohamed%2BSohaimi.pdf
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author Norfitriah Mohamed Sohaimi,
Mohd Hair Bejo,
author_facet Norfitriah Mohamed Sohaimi,
Mohd Hair Bejo,
author_sort Norfitriah Mohamed Sohaimi,
building UKM Institutional Repository
collection Online Access
description Fowl adenovirus (FAdV) is the primary agent of inclusion body hepatitis (IBH) in poultry and caused serious economic impact due to high mortality and poor productivity. To date, clinical cases of IBH in chickens increases over the years in Malaysia, thus, an effective local vaccine to control the disease outbreak is in dire need. The objective of the study was to determine the efficacy of live attenuated FAdV serotype 8b isolate, UPM1137CEL35, in specific-pathogen-free chickens. Attenuated isolate UPM1137CEL35 conferred full protection against the virulent FAdV isolate, UPM11134 in the vaccinated chickens. Neither clinical signs nor mortality were recorded in all vaccinated groups. However, in the challenged unvaccinated group, chickens showed clinical signs of weakness, reduced feed consumption, and lateral recumbency at day 4 to 7 postchallenge. Additionally, the body weight was low significantly (p<0.05) compared to the challenged vaccinated groups. Upon necropsy, the liver from the challenged unvaccinated group was pale at peripheral bilateral lobes with the presence of focal lymphoid aggregation microscopically, while, challenged vaccinated groups were normal without significant changes. All vaccinated chickens were protected from disease manifestations with antibody response compared to the challenged unvaccinated group. It was concluded that the attenuated FAdV isolate, UPM1137CEL35, has a high potential to be used as a vaccine against the FAdV serotype 8b of Malaysian isolates.
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spelling oai:generic.eprints.org:188922022-07-05T07:51:50Z http://journalarticle.ukm.my/18892/ Efficacy of live attenuated fowl adenovirus serotype 8B isolate of Malaysia in specific pathogen-free chickens Norfitriah Mohamed Sohaimi, Mohd Hair Bejo, Fowl adenovirus (FAdV) is the primary agent of inclusion body hepatitis (IBH) in poultry and caused serious economic impact due to high mortality and poor productivity. To date, clinical cases of IBH in chickens increases over the years in Malaysia, thus, an effective local vaccine to control the disease outbreak is in dire need. The objective of the study was to determine the efficacy of live attenuated FAdV serotype 8b isolate, UPM1137CEL35, in specific-pathogen-free chickens. Attenuated isolate UPM1137CEL35 conferred full protection against the virulent FAdV isolate, UPM11134 in the vaccinated chickens. Neither clinical signs nor mortality were recorded in all vaccinated groups. However, in the challenged unvaccinated group, chickens showed clinical signs of weakness, reduced feed consumption, and lateral recumbency at day 4 to 7 postchallenge. Additionally, the body weight was low significantly (p<0.05) compared to the challenged vaccinated groups. Upon necropsy, the liver from the challenged unvaccinated group was pale at peripheral bilateral lobes with the presence of focal lymphoid aggregation microscopically, while, challenged vaccinated groups were normal without significant changes. All vaccinated chickens were protected from disease manifestations with antibody response compared to the challenged unvaccinated group. It was concluded that the attenuated FAdV isolate, UPM1137CEL35, has a high potential to be used as a vaccine against the FAdV serotype 8b of Malaysian isolates. Penerbit Universiti Kebangsaan Malaysia 2021 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18892/1/135-143%2B%2B%2BNorfitriah%2BMohamed%2BSohaimi.pdf Norfitriah Mohamed Sohaimi, and Mohd Hair Bejo, (2021) Efficacy of live attenuated fowl adenovirus serotype 8B isolate of Malaysia in specific pathogen-free chickens. Malaysian Applied Biology, 50 (3). pp. 135-143. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/43
spellingShingle Norfitriah Mohamed Sohaimi,
Mohd Hair Bejo,
Efficacy of live attenuated fowl adenovirus serotype 8B isolate of Malaysia in specific pathogen-free chickens
title Efficacy of live attenuated fowl adenovirus serotype 8B isolate of Malaysia in specific pathogen-free chickens
title_full Efficacy of live attenuated fowl adenovirus serotype 8B isolate of Malaysia in specific pathogen-free chickens
title_fullStr Efficacy of live attenuated fowl adenovirus serotype 8B isolate of Malaysia in specific pathogen-free chickens
title_full_unstemmed Efficacy of live attenuated fowl adenovirus serotype 8B isolate of Malaysia in specific pathogen-free chickens
title_short Efficacy of live attenuated fowl adenovirus serotype 8B isolate of Malaysia in specific pathogen-free chickens
title_sort efficacy of live attenuated fowl adenovirus serotype 8b isolate of malaysia in specific pathogen-free chickens
url http://journalarticle.ukm.my/18892/
http://journalarticle.ukm.my/18892/
http://journalarticle.ukm.my/18892/1/135-143%2B%2B%2BNorfitriah%2BMohamed%2BSohaimi.pdf