Combination effect of tamoxifen and ascorbic acid treatment on breast cancer cells (MCF-7) and cervical cancer cells (HeLa)
Breast cancer and cervical cancer are among the leading causes of death among women in the world. Even though chemotherapy is available as cancer treatment, the development of drug resistance in both cancer cells has reduced the efficacy of chemotherapeutic drugs in such treatment. The current study...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/18591/ http://journalarticle.ukm.my/18591/1/38960-160192-1-PB.pdf |
| _version_ | 1848814614547005440 |
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| author | Hasmah Abdullah, Norlida Mamat, Nor Munirah Zakaria, Nur Iman Fatihah Mohd Yunan, Muhammad Irfan Noor Hisham, Hermizi Hapidin, |
| author_facet | Hasmah Abdullah, Norlida Mamat, Nor Munirah Zakaria, Nur Iman Fatihah Mohd Yunan, Muhammad Irfan Noor Hisham, Hermizi Hapidin, |
| author_sort | Hasmah Abdullah, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Breast cancer and cervical cancer are among the leading causes of death among women in the world. Even though chemotherapy is available as cancer treatment, the development of drug resistance in both cancer cells has reduced the efficacy of chemotherapeutic drugs in such treatment. The current study was aimed to evaluate the cell viability of human breast cancer cells, MCF-7, and cervical cancer cells, HeLa upon the combination treatment of ascorbic acid and tamoxifen. The cell viability was measured using the MTT assay, with an incubation period of 72 hours in a humidified CO2 incubator. The concentrations of tamoxifen and ascorbic acid that reduced 50% of the cell population (IC50) were determined from the dose-response curve. The IC50 concentration was used to determine the cell viability in the treated cells. CompuSyn software was used to evaluate the combined effects towards both cells upon treatment and the results were calculated as combination index (CI). The data were analyzed using GraphPad Prism (version 7). Statistical analysis was performed using an independent t-test. The IC50 values of tamoxifen and ascorbic acid on MCF-7 cells were 14.53 μg/ml and 15.8 μg/ml respectively, while the IC50 values of tamoxifen and ascorbic acid on HeLa cells were 11.09 μg/ml and 202.3 μg/ml respectively. The combination of tamoxifen and ascorbic acid exerted a greater growth reduction percentage in both cells compared to tamoxifen alone. The results indicated that ascorbic acid synergizes the cytotoxic effect of tamoxifen at lower concentrations towards MCF-7 cells with a CI less than 1. However, the combination of tamoxifen and ascorbic acid exerted an antagonistic effect in HeLa cells, with a CI more than 1. |
| first_indexed | 2025-11-15T00:36:53Z |
| format | Article |
| id | oai:generic.eprints.org:18591 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:36:53Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:185912022-05-17T06:43:48Z http://journalarticle.ukm.my/18591/ Combination effect of tamoxifen and ascorbic acid treatment on breast cancer cells (MCF-7) and cervical cancer cells (HeLa) Hasmah Abdullah, Norlida Mamat, Nor Munirah Zakaria, Nur Iman Fatihah Mohd Yunan, Muhammad Irfan Noor Hisham, Hermizi Hapidin, Breast cancer and cervical cancer are among the leading causes of death among women in the world. Even though chemotherapy is available as cancer treatment, the development of drug resistance in both cancer cells has reduced the efficacy of chemotherapeutic drugs in such treatment. The current study was aimed to evaluate the cell viability of human breast cancer cells, MCF-7, and cervical cancer cells, HeLa upon the combination treatment of ascorbic acid and tamoxifen. The cell viability was measured using the MTT assay, with an incubation period of 72 hours in a humidified CO2 incubator. The concentrations of tamoxifen and ascorbic acid that reduced 50% of the cell population (IC50) were determined from the dose-response curve. The IC50 concentration was used to determine the cell viability in the treated cells. CompuSyn software was used to evaluate the combined effects towards both cells upon treatment and the results were calculated as combination index (CI). The data were analyzed using GraphPad Prism (version 7). Statistical analysis was performed using an independent t-test. The IC50 values of tamoxifen and ascorbic acid on MCF-7 cells were 14.53 μg/ml and 15.8 μg/ml respectively, while the IC50 values of tamoxifen and ascorbic acid on HeLa cells were 11.09 μg/ml and 202.3 μg/ml respectively. The combination of tamoxifen and ascorbic acid exerted a greater growth reduction percentage in both cells compared to tamoxifen alone. The results indicated that ascorbic acid synergizes the cytotoxic effect of tamoxifen at lower concentrations towards MCF-7 cells with a CI less than 1. However, the combination of tamoxifen and ascorbic acid exerted an antagonistic effect in HeLa cells, with a CI more than 1. Penerbit Universiti Kebangsaan Malaysia 2021 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18591/1/38960-160192-1-PB.pdf Hasmah Abdullah, and Norlida Mamat, and Nor Munirah Zakaria, and Nur Iman Fatihah Mohd Yunan, and Muhammad Irfan Noor Hisham, and Hermizi Hapidin, (2021) Combination effect of tamoxifen and ascorbic acid treatment on breast cancer cells (MCF-7) and cervical cancer cells (HeLa). Jurnal Sains Kesihatan Malaysia, 19 (2). pp. 104-114. ISSN 1675-8161 https://ejournal.ukm.my/jskm/issue/view/1355 |
| spellingShingle | Hasmah Abdullah, Norlida Mamat, Nor Munirah Zakaria, Nur Iman Fatihah Mohd Yunan, Muhammad Irfan Noor Hisham, Hermizi Hapidin, Combination effect of tamoxifen and ascorbic acid treatment on breast cancer cells (MCF-7) and cervical cancer cells (HeLa) |
| title | Combination effect of tamoxifen and ascorbic acid treatment on breast cancer cells (MCF-7) and cervical cancer cells (HeLa) |
| title_full | Combination effect of tamoxifen and ascorbic acid treatment on breast cancer cells (MCF-7) and cervical cancer cells (HeLa) |
| title_fullStr | Combination effect of tamoxifen and ascorbic acid treatment on breast cancer cells (MCF-7) and cervical cancer cells (HeLa) |
| title_full_unstemmed | Combination effect of tamoxifen and ascorbic acid treatment on breast cancer cells (MCF-7) and cervical cancer cells (HeLa) |
| title_short | Combination effect of tamoxifen and ascorbic acid treatment on breast cancer cells (MCF-7) and cervical cancer cells (HeLa) |
| title_sort | combination effect of tamoxifen and ascorbic acid treatment on breast cancer cells (mcf-7) and cervical cancer cells (hela) |
| url | http://journalarticle.ukm.my/18591/ http://journalarticle.ukm.my/18591/ http://journalarticle.ukm.my/18591/1/38960-160192-1-PB.pdf |