A safety evaluation of intravenous administration of ex vivo expanded human peripheral blood-derived NK cells : a preclinical study
The use of natural killer (NK) cells in the treatment of various cancers is emerging as a promising approach in adoptive immunotherapy. However, the safety of ex vivo activated and expanded cells in in vivo conditions remain unknown. In this study, the toxicity of NK cells was evaluated at different...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/18364/ http://journalarticle.ukm.my/18364/1/22.pdf |
| _version_ | 1848814553239912448 |
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| author | Sellamuthu Subbanna Gounder, Baskar Subramani, Nur Ezzati Izyan Mohd Radzuanb, Nurhidayah Mohamad Sait, Farah Dalila Mohd Zain, Basri Johan Jeet Abdullah, |
| author_facet | Sellamuthu Subbanna Gounder, Baskar Subramani, Nur Ezzati Izyan Mohd Radzuanb, Nurhidayah Mohamad Sait, Farah Dalila Mohd Zain, Basri Johan Jeet Abdullah, |
| author_sort | Sellamuthu Subbanna Gounder, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The use of natural killer (NK) cells in the treatment of various cancers is emerging as a promising approach in adoptive immunotherapy. However, the safety of ex vivo activated and expanded cells in in vivo conditions remain unknown. In this study, the toxicity of NK cells was evaluated at different doses, with 5 × 106, 20 × 106 and 50 × 106 cells injected intravenously into pre-irradiated (30Gy) immunodeficient mice twice a week for three weeks and the mice were followed-up on for 90 days. Throughout the study, no mortality, abnormal clinical signs, or behavioural changes related to the testing material were observed in either the treated or control groups of mice. There were no significant variations in food and water consumption between both genders in the NK cell treated and control groups. However, certain significant changes were observed between the groups in the clinical biochemistry and urine analysis reports. As autopsy showed no significant variations in absolute and relative organ weights between the groups, except for the livers of the treated mice. The histopathological analysis also demonstrated that there were no significant abnormalities in most of the organs of both genders, except for the liver. Some necrotic lesions were observed in the livers of both the treated and control mice, and these lesions may be due to the effects of irradiation or could be common in NOD.SCID mice. The findings of this study indicate that intravenous administration of NK cells is safe and does not cause any adverse effects up to the dose of 50 × 106 cells/mouse. |
| first_indexed | 2025-11-15T00:35:55Z |
| format | Article |
| id | oai:generic.eprints.org:18364 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:35:55Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:183642022-04-11T07:27:44Z http://journalarticle.ukm.my/18364/ A safety evaluation of intravenous administration of ex vivo expanded human peripheral blood-derived NK cells : a preclinical study Sellamuthu Subbanna Gounder, Baskar Subramani, Nur Ezzati Izyan Mohd Radzuanb, Nurhidayah Mohamad Sait, Farah Dalila Mohd Zain, Basri Johan Jeet Abdullah, The use of natural killer (NK) cells in the treatment of various cancers is emerging as a promising approach in adoptive immunotherapy. However, the safety of ex vivo activated and expanded cells in in vivo conditions remain unknown. In this study, the toxicity of NK cells was evaluated at different doses, with 5 × 106, 20 × 106 and 50 × 106 cells injected intravenously into pre-irradiated (30Gy) immunodeficient mice twice a week for three weeks and the mice were followed-up on for 90 days. Throughout the study, no mortality, abnormal clinical signs, or behavioural changes related to the testing material were observed in either the treated or control groups of mice. There were no significant variations in food and water consumption between both genders in the NK cell treated and control groups. However, certain significant changes were observed between the groups in the clinical biochemistry and urine analysis reports. As autopsy showed no significant variations in absolute and relative organ weights between the groups, except for the livers of the treated mice. The histopathological analysis also demonstrated that there were no significant abnormalities in most of the organs of both genders, except for the liver. Some necrotic lesions were observed in the livers of both the treated and control mice, and these lesions may be due to the effects of irradiation or could be common in NOD.SCID mice. The findings of this study indicate that intravenous administration of NK cells is safe and does not cause any adverse effects up to the dose of 50 × 106 cells/mouse. Penerbit Universiti Kebangsaan Malaysia 2022-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18364/1/22.pdf Sellamuthu Subbanna Gounder, and Baskar Subramani, and Nur Ezzati Izyan Mohd Radzuanb, and Nurhidayah Mohamad Sait, and Farah Dalila Mohd Zain, and Basri Johan Jeet Abdullah, (2022) A safety evaluation of intravenous administration of ex vivo expanded human peripheral blood-derived NK cells : a preclinical study. Sains Malaysiana, 51 (1). pp. 271-284. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil1_2022/KandunganJilid51Bil1_2022.html |
| spellingShingle | Sellamuthu Subbanna Gounder, Baskar Subramani, Nur Ezzati Izyan Mohd Radzuanb, Nurhidayah Mohamad Sait, Farah Dalila Mohd Zain, Basri Johan Jeet Abdullah, A safety evaluation of intravenous administration of ex vivo expanded human peripheral blood-derived NK cells : a preclinical study |
| title | A safety evaluation of intravenous administration of ex vivo expanded human peripheral blood-derived NK cells : a preclinical study |
| title_full | A safety evaluation of intravenous administration of ex vivo expanded human peripheral blood-derived NK cells : a preclinical study |
| title_fullStr | A safety evaluation of intravenous administration of ex vivo expanded human peripheral blood-derived NK cells : a preclinical study |
| title_full_unstemmed | A safety evaluation of intravenous administration of ex vivo expanded human peripheral blood-derived NK cells : a preclinical study |
| title_short | A safety evaluation of intravenous administration of ex vivo expanded human peripheral blood-derived NK cells : a preclinical study |
| title_sort | safety evaluation of intravenous administration of ex vivo expanded human peripheral blood-derived nk cells : a preclinical study |
| url | http://journalarticle.ukm.my/18364/ http://journalarticle.ukm.my/18364/ http://journalarticle.ukm.my/18364/1/22.pdf |