Engineering Lactococcus lactis as a cell factory for the production of limonene
Limonene is a plant monoterpene which contributes significantly to the scent of most essential oils due to its pleasant fragrance. The compound had been reported to have anti-cancer properties against several types of cancer including colorectal cancer. However, the production of this compound in na...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/18350/ http://journalarticle.ukm.my/18350/1/8.pdf |
| _version_ | 1848814549114814464 |
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| author | Nurul ‘Aishah Shaili, Song, Adelene Ai-Lian Sarah Othman, Lian, Lionel Aun In Janna Ong-Abdullah, Raha Abdul Rahim, |
| author_facet | Nurul ‘Aishah Shaili, Song, Adelene Ai-Lian Sarah Othman, Lian, Lionel Aun In Janna Ong-Abdullah, Raha Abdul Rahim, |
| author_sort | Nurul ‘Aishah Shaili, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Limonene is a plant monoterpene which contributes significantly to the scent of most essential oils due to its pleasant fragrance. The compound had been reported to have anti-cancer properties against several types of cancer including colorectal cancer. However, the production of this compound in nature is limited because it is produced as a secondary metabolite. To overcome these challenges, Lactococcus lactis was developed as a heterologous host for the production of limonene. A synthesized limonene synthase (LS) from Mentha spicata (mint) was cloned into L. lactis NZ9000. Western blot analysis using mouse IgG His-Tag monoclonal antibody showed successful LS expression by L. lactis at the size of ~55 kDa. GC-MS analysis results showed that limonene production was optimum after 24 h of induction (~8.0 ppm). Metabolic engineering was attempted to enhance the limonene production by overexpression of lactococcal 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) and mevalonate kinase (mvk) genes in the bacterial host. The recombinant L. lactis carrying pNZ:LSMM plasmid successfully enhanced the limonene production to two-fold (~15.1 ppm) after 24 h of induction. The outcomes of this study show the potential of L. lactis to produce plant proteins and bioactive compounds production, which prospectively leads to an oral delivery system for anti-cancer compounds. |
| first_indexed | 2025-11-15T00:35:51Z |
| format | Article |
| id | oai:generic.eprints.org:18350 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:35:51Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:183502022-04-11T06:17:05Z http://journalarticle.ukm.my/18350/ Engineering Lactococcus lactis as a cell factory for the production of limonene Nurul ‘Aishah Shaili, Song, Adelene Ai-Lian Sarah Othman, Lian, Lionel Aun In Janna Ong-Abdullah, Raha Abdul Rahim, Limonene is a plant monoterpene which contributes significantly to the scent of most essential oils due to its pleasant fragrance. The compound had been reported to have anti-cancer properties against several types of cancer including colorectal cancer. However, the production of this compound in nature is limited because it is produced as a secondary metabolite. To overcome these challenges, Lactococcus lactis was developed as a heterologous host for the production of limonene. A synthesized limonene synthase (LS) from Mentha spicata (mint) was cloned into L. lactis NZ9000. Western blot analysis using mouse IgG His-Tag monoclonal antibody showed successful LS expression by L. lactis at the size of ~55 kDa. GC-MS analysis results showed that limonene production was optimum after 24 h of induction (~8.0 ppm). Metabolic engineering was attempted to enhance the limonene production by overexpression of lactococcal 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) and mevalonate kinase (mvk) genes in the bacterial host. The recombinant L. lactis carrying pNZ:LSMM plasmid successfully enhanced the limonene production to two-fold (~15.1 ppm) after 24 h of induction. The outcomes of this study show the potential of L. lactis to produce plant proteins and bioactive compounds production, which prospectively leads to an oral delivery system for anti-cancer compounds. Penerbit Universiti Kebangsaan Malaysia 2022-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18350/1/8.pdf Nurul ‘Aishah Shaili, and Song, Adelene Ai-Lian and Sarah Othman, and Lian, Lionel Aun In and Janna Ong-Abdullah, and Raha Abdul Rahim, (2022) Engineering Lactococcus lactis as a cell factory for the production of limonene. Sains Malaysiana, 51 (1). pp. 95-105. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil1_2022/KandunganJilid51Bil1_2022.html |
| spellingShingle | Nurul ‘Aishah Shaili, Song, Adelene Ai-Lian Sarah Othman, Lian, Lionel Aun In Janna Ong-Abdullah, Raha Abdul Rahim, Engineering Lactococcus lactis as a cell factory for the production of limonene |
| title | Engineering Lactococcus lactis as a cell factory for the production of limonene |
| title_full | Engineering Lactococcus lactis as a cell factory for the production of limonene |
| title_fullStr | Engineering Lactococcus lactis as a cell factory for the production of limonene |
| title_full_unstemmed | Engineering Lactococcus lactis as a cell factory for the production of limonene |
| title_short | Engineering Lactococcus lactis as a cell factory for the production of limonene |
| title_sort | engineering lactococcus lactis as a cell factory for the production of limonene |
| url | http://journalarticle.ukm.my/18350/ http://journalarticle.ukm.my/18350/ http://journalarticle.ukm.my/18350/1/8.pdf |