Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model
Fluoxetine, a selective serotonin reuptake inhibitor anti-depressant, causes undesirable side effects, including diarrhea and constipation. This research investigated the direct effects of fluoxetine at 0.001, 0.01, 0.1, 1, 10, and 100 μM on duodenal and proximal colonic tissue contractions. The inv...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/18332/ http://journalarticle.ukm.my/18332/1/15.pdf |
| _version_ | 1848814543989374976 |
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| author | Pissared Khuituan, Chotika Nhaemchei, Sakda Pradab, Sakena K-da, Nipaporn Konthapakdee, |
| author_facet | Pissared Khuituan, Chotika Nhaemchei, Sakda Pradab, Sakena K-da, Nipaporn Konthapakdee, |
| author_sort | Pissared Khuituan, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Fluoxetine, a selective serotonin reuptake inhibitor anti-depressant, causes undesirable side effects, including diarrhea and constipation. This research investigated the direct effects of fluoxetine at 0.001, 0.01, 0.1, 1, 10, and 100 μM on duodenal and proximal colonic tissue contractions. The investigation aimed to determine related mechanisms using an isolated mouse intestine model. Our study showed that fluoxetine at 0.001 μM increased the amplitude of contraction in colonic tissue but decreased the amplitude in duodenal tissue. The direct application of higher concentrations of fluoxetine (1, 10, and 100 μM) reduced the amplitude of contractions in proximal colonic tissue. Moreover, we found that the stimulatory effect of 0.001 μM fluoxetine on the tone of contractions could be prevented by pre-incubating the tissue in ondansetron and atropine. Our findings suggest that the inhibition of the effect of fluoxetine was mainly mediated via 5-HT3 receptors and muscarinic signaling. These findings might explain the conflicting gastrointestinal symptoms caused by fluoxetine. |
| first_indexed | 2025-11-15T00:35:46Z |
| format | Article |
| id | oai:generic.eprints.org:18332 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:35:46Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:183322022-04-11T04:54:45Z http://journalarticle.ukm.my/18332/ Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model Pissared Khuituan, Chotika Nhaemchei, Sakda Pradab, Sakena K-da, Nipaporn Konthapakdee, Fluoxetine, a selective serotonin reuptake inhibitor anti-depressant, causes undesirable side effects, including diarrhea and constipation. This research investigated the direct effects of fluoxetine at 0.001, 0.01, 0.1, 1, 10, and 100 μM on duodenal and proximal colonic tissue contractions. The investigation aimed to determine related mechanisms using an isolated mouse intestine model. Our study showed that fluoxetine at 0.001 μM increased the amplitude of contraction in colonic tissue but decreased the amplitude in duodenal tissue. The direct application of higher concentrations of fluoxetine (1, 10, and 100 μM) reduced the amplitude of contractions in proximal colonic tissue. Moreover, we found that the stimulatory effect of 0.001 μM fluoxetine on the tone of contractions could be prevented by pre-incubating the tissue in ondansetron and atropine. Our findings suggest that the inhibition of the effect of fluoxetine was mainly mediated via 5-HT3 receptors and muscarinic signaling. These findings might explain the conflicting gastrointestinal symptoms caused by fluoxetine. Penerbit Universiti Kebangsaan Malaysia 2021-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18332/1/15.pdf Pissared Khuituan, and Chotika Nhaemchei, and Sakda Pradab, and Sakena K-da, and Nipaporn Konthapakdee, (2021) Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model. Sains Malaysiana, 50 (12). pp. 3647-3657. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil12_2021/KandunganJilid50Bil12_2021.html |
| spellingShingle | Pissared Khuituan, Chotika Nhaemchei, Sakda Pradab, Sakena K-da, Nipaporn Konthapakdee, Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model |
| title | Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model |
| title_full | Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model |
| title_fullStr | Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model |
| title_full_unstemmed | Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model |
| title_short | Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model |
| title_sort | fluoxetine affects intestinal motility via 5-ht3 and muscarinic receptors in ex vivo mouse model |
| url | http://journalarticle.ukm.my/18332/ http://journalarticle.ukm.my/18332/ http://journalarticle.ukm.my/18332/1/15.pdf |