Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies
The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is the optimization step, which involves the depletion of high abundant proteins from the samples. The objective of this study was to develop a cost efficient and effective method to remove albumin from canin...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/18302/ http://journalarticle.ukm.my/18302/1/13.pdf |
| _version_ | 1848814536012857344 |
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| author | Tan, Wei Miao Lau, Seng Fong Sharifah Zakiah Syed Sulaiman, Nor Shahira Solehah Umran, Mokrish Ajat, Rozaihan Mansor, Puteri Azaziah Megat Abd-Rani, Intan NurFatiha Shafie, Ng, Angela Min Hwei Norasfaliza Rahmad, |
| author_facet | Tan, Wei Miao Lau, Seng Fong Sharifah Zakiah Syed Sulaiman, Nor Shahira Solehah Umran, Mokrish Ajat, Rozaihan Mansor, Puteri Azaziah Megat Abd-Rani, Intan NurFatiha Shafie, Ng, Angela Min Hwei Norasfaliza Rahmad, |
| author_sort | Tan, Wei Miao |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is the optimization step, which involves the depletion of high abundant proteins from the samples. The objective of this study was to develop a cost efficient and effective method to remove albumin from canine synovial fluid for proteome studies. Pooled synovial fluid samples were obtained from clinically healthy dogs (n=5), with no radiographic features of osteoarthritis. The acetone precipitation method and 10% w/v of trichloroacetic acid/acetone were chosen to deplete the albumin from canine synovial fluid and the outcome from the different methods were compared using one dimensional and two-dimensional gel electrophoresis studies. The results showed that the 10% w/v TCA/acetone precipitation method removed highly abundant proteins from synovial fluid for gel electrophoresis studies compared to the acetone precipitation method. The 10% w/v TCA/acetone precipitation method provides an effective method to remove albumin from the synovial fluid using gel electrophoresis, especially two-dimensional gel electrophoresis. The accessibility and cost of TCA and acetone make this method of precipitation a simple and cost-effective technique in preparing a canine synovial fluid for two-dimensional gel electrophoresis analysis. |
| first_indexed | 2025-11-15T00:35:38Z |
| format | Article |
| id | oai:generic.eprints.org:18302 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:35:38Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:183022022-04-06T06:14:55Z http://journalarticle.ukm.my/18302/ Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies Tan, Wei Miao Lau, Seng Fong Sharifah Zakiah Syed Sulaiman, Nor Shahira Solehah Umran, Mokrish Ajat, Rozaihan Mansor, Puteri Azaziah Megat Abd-Rani, Intan NurFatiha Shafie, Ng, Angela Min Hwei Norasfaliza Rahmad, The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is the optimization step, which involves the depletion of high abundant proteins from the samples. The objective of this study was to develop a cost efficient and effective method to remove albumin from canine synovial fluid for proteome studies. Pooled synovial fluid samples were obtained from clinically healthy dogs (n=5), with no radiographic features of osteoarthritis. The acetone precipitation method and 10% w/v of trichloroacetic acid/acetone were chosen to deplete the albumin from canine synovial fluid and the outcome from the different methods were compared using one dimensional and two-dimensional gel electrophoresis studies. The results showed that the 10% w/v TCA/acetone precipitation method removed highly abundant proteins from synovial fluid for gel electrophoresis studies compared to the acetone precipitation method. The 10% w/v TCA/acetone precipitation method provides an effective method to remove albumin from the synovial fluid using gel electrophoresis, especially two-dimensional gel electrophoresis. The accessibility and cost of TCA and acetone make this method of precipitation a simple and cost-effective technique in preparing a canine synovial fluid for two-dimensional gel electrophoresis analysis. Penerbit Universiti Kebangsaan Malaysia 2021-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18302/1/13.pdf Tan, Wei Miao and Lau, Seng Fong and Sharifah Zakiah Syed Sulaiman, and Nor Shahira Solehah Umran, and Mokrish Ajat, and Rozaihan Mansor, and Puteri Azaziah Megat Abd-Rani, and Intan NurFatiha Shafie, and Ng, Angela Min Hwei and Norasfaliza Rahmad, (2021) Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies. Sains Malaysiana, 50 (11). pp. 3297-3302. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil11_2021/KandunganJilid50Bil11_2021.html |
| spellingShingle | Tan, Wei Miao Lau, Seng Fong Sharifah Zakiah Syed Sulaiman, Nor Shahira Solehah Umran, Mokrish Ajat, Rozaihan Mansor, Puteri Azaziah Megat Abd-Rani, Intan NurFatiha Shafie, Ng, Angela Min Hwei Norasfaliza Rahmad, Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title | Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title_full | Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title_fullStr | Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title_full_unstemmed | Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title_short | Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title_sort | trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| url | http://journalarticle.ukm.my/18302/ http://journalarticle.ukm.my/18302/ http://journalarticle.ukm.my/18302/1/13.pdf |