Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography
Poly(ethylene oxide) (PEO) bonded stationary phase has been synthesized by a single and simple step reaction. Poly(ethylene glycol monomethyl ether p-toluene sulfonate) (tosylated-PEO, molecular weight 900, n ≈ 18) was chemically bonded to 3-aminopropyl silica (TSKgel NH2-60, 5 μm particle size, and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/18296/ http://journalarticle.ukm.my/18296/1/8.pdf |
| _version_ | 1848814534554288128 |
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| author | Roza Linda, Abdullah, Mohamad Rafi, Aster Rahayu, Lee, Lee Wah Takeuchi, Toyohide |
| author_facet | Roza Linda, Abdullah, Mohamad Rafi, Aster Rahayu, Lee, Lee Wah Takeuchi, Toyohide |
| author_sort | Roza Linda, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Poly(ethylene oxide) (PEO) bonded stationary phase has been synthesized by a single and simple step reaction. Poly(ethylene glycol monomethyl ether p-toluene sulfonate) (tosylated-PEO, molecular weight 900, n ≈ 18) was chemically bonded to 3-aminopropyl silica (TSKgel NH2-60, 5 μm particle size, and 60 Å mean pore diameter). The prepared stationary phase was able to separate polar compounds such as phenolics and nucleobases in capillary liquid chromatography. The retention and separation of phenolics and nucleobases could be achieved under isocratic elution condition. Nucleobases such as thymine, adenine, uracil, uridine, cytidine and toluene and phenolics (phenol, pyrocatechol, pyrogallol) were baseline separated in less than 6 min using 98% acetonitrile and less than 7 minutes using 80% acetonitrile, respectively. We demonstrated that the retention of nucleobases as analyte decreased with decreasing eluent concentration. The retention of these polar compounds was believed to be based on dipole-dipole and/or hydrogen-bonding interactions. |
| first_indexed | 2025-11-15T00:35:37Z |
| format | Article |
| id | oai:generic.eprints.org:18296 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:35:37Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:182962022-04-06T06:09:46Z http://journalarticle.ukm.my/18296/ Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography Roza Linda, Abdullah, Mohamad Rafi, Aster Rahayu, Lee, Lee Wah Takeuchi, Toyohide Poly(ethylene oxide) (PEO) bonded stationary phase has been synthesized by a single and simple step reaction. Poly(ethylene glycol monomethyl ether p-toluene sulfonate) (tosylated-PEO, molecular weight 900, n ≈ 18) was chemically bonded to 3-aminopropyl silica (TSKgel NH2-60, 5 μm particle size, and 60 Å mean pore diameter). The prepared stationary phase was able to separate polar compounds such as phenolics and nucleobases in capillary liquid chromatography. The retention and separation of phenolics and nucleobases could be achieved under isocratic elution condition. Nucleobases such as thymine, adenine, uracil, uridine, cytidine and toluene and phenolics (phenol, pyrocatechol, pyrogallol) were baseline separated in less than 6 min using 98% acetonitrile and less than 7 minutes using 80% acetonitrile, respectively. We demonstrated that the retention of nucleobases as analyte decreased with decreasing eluent concentration. The retention of these polar compounds was believed to be based on dipole-dipole and/or hydrogen-bonding interactions. Penerbit Universiti Kebangsaan Malaysia 2021-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18296/1/8.pdf Roza Linda, and Abdullah, and Mohamad Rafi, and Aster Rahayu, and Lee, Lee Wah and Takeuchi, Toyohide (2021) Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography. Sains Malaysiana, 50 (11). pp. 3241-3250. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil11_2021/KandunganJilid50Bil11_2021.html |
| spellingShingle | Roza Linda, Abdullah, Mohamad Rafi, Aster Rahayu, Lee, Lee Wah Takeuchi, Toyohide Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography |
| title | Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography |
| title_full | Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography |
| title_fullStr | Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography |
| title_full_unstemmed | Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography |
| title_short | Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography |
| title_sort | separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography |
| url | http://journalarticle.ukm.my/18296/ http://journalarticle.ukm.my/18296/ http://journalarticle.ukm.my/18296/1/8.pdf |