Cardiac depressant and vasodialatory effect of flaxseed - basis for the medicinal use in hypertension
Linum usitatissimum (Flaxseed) is known to be traditionally used for managing hypertension. In this study, we aim to provide a mechanistic basis for the medicinal use of Flaxseed in hypertension. The high-performance liquid chromatography (HPLC) analysis that we carried out during our study showed t...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/18065/ http://journalarticle.ukm.my/18065/1/16.pdf |
| _version_ | 1848814474010558464 |
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| author | Palla, Amber Hanif Ur Rehman, Najeeb Siddiqi, Hasan Salman Gilani, Anwar-Ul-Hassan Siddiqui, Bina Shaheen Ilyas, Maimoona Perwaiz, Sobiya |
| author_facet | Palla, Amber Hanif Ur Rehman, Najeeb Siddiqi, Hasan Salman Gilani, Anwar-Ul-Hassan Siddiqui, Bina Shaheen Ilyas, Maimoona Perwaiz, Sobiya |
| author_sort | Palla, Amber Hanif |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Linum usitatissimum (Flaxseed) is known to be traditionally used for managing hypertension. In this study, we aim to provide a mechanistic basis for the medicinal use of Flaxseed in hypertension. The high-performance liquid chromatography (HPLC) analysis that we carried out during our study showed the presence of polar compounds (quercetin, nicotinic acid, and nicotinamide) in Flaxseed’s crude extract (Fs.Cr; aqueous methanolic). In anesthetized rats, Fs.Cr reduced arterial blood pressure (BP) dose-dependently (10-100 mg/kg). When tested for its mechanism of action ex vivo, Fs.Cr inhibited both the force and rate of spontaneous contractions in the dose range of 1-10 mg/mL in isolated guinea-pig atria, similar to how verapamil, a standard Ca+2 channel blocker does it. Further, Fs.Cr showed vasodilator effect against the contractions induced by phenylephrine (PE, 1 μM) in rat aortic ring preparations (concentration range: 1-10 mg/mL), whereas no effect was observed against the contractions induced by low K+ (25 mM) as well as high K+ (80 mM). This selective inhibitory effect of Fs.Cr against PE was tested for endothelium-dependent nitric oxide (NO) and/or cholinergic component involvement. The vasodilator effect of Fs.Cr against PE was retested in the absence and presence of atropine in endothelium (E)-intact and E-denuded aorta, but no significant shift was observed in the inhibitory effect of Fs.Cr. Further, Fs.Cr shifted the PE-induced concentration-response curves (CRCs) to the right in a dose-dependent manner (1 and 3 mg/mL). This effect was similar to that of prazosin. All these findings indicate that Flaxseed may mediate its antihypertensive activity by the alpha-1 receptor antagonist and Ca+2 channel blocking-like activity, which may account for its efficacy in hypertension. |
| first_indexed | 2025-11-15T00:34:39Z |
| format | Article |
| id | oai:generic.eprints.org:18065 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:34:39Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:180652022-02-18T00:51:59Z http://journalarticle.ukm.my/18065/ Cardiac depressant and vasodialatory effect of flaxseed - basis for the medicinal use in hypertension Palla, Amber Hanif Ur Rehman, Najeeb Siddiqi, Hasan Salman Gilani, Anwar-Ul-Hassan Siddiqui, Bina Shaheen Ilyas, Maimoona Perwaiz, Sobiya Linum usitatissimum (Flaxseed) is known to be traditionally used for managing hypertension. In this study, we aim to provide a mechanistic basis for the medicinal use of Flaxseed in hypertension. The high-performance liquid chromatography (HPLC) analysis that we carried out during our study showed the presence of polar compounds (quercetin, nicotinic acid, and nicotinamide) in Flaxseed’s crude extract (Fs.Cr; aqueous methanolic). In anesthetized rats, Fs.Cr reduced arterial blood pressure (BP) dose-dependently (10-100 mg/kg). When tested for its mechanism of action ex vivo, Fs.Cr inhibited both the force and rate of spontaneous contractions in the dose range of 1-10 mg/mL in isolated guinea-pig atria, similar to how verapamil, a standard Ca+2 channel blocker does it. Further, Fs.Cr showed vasodilator effect against the contractions induced by phenylephrine (PE, 1 μM) in rat aortic ring preparations (concentration range: 1-10 mg/mL), whereas no effect was observed against the contractions induced by low K+ (25 mM) as well as high K+ (80 mM). This selective inhibitory effect of Fs.Cr against PE was tested for endothelium-dependent nitric oxide (NO) and/or cholinergic component involvement. The vasodilator effect of Fs.Cr against PE was retested in the absence and presence of atropine in endothelium (E)-intact and E-denuded aorta, but no significant shift was observed in the inhibitory effect of Fs.Cr. Further, Fs.Cr shifted the PE-induced concentration-response curves (CRCs) to the right in a dose-dependent manner (1 and 3 mg/mL). This effect was similar to that of prazosin. All these findings indicate that Flaxseed may mediate its antihypertensive activity by the alpha-1 receptor antagonist and Ca+2 channel blocking-like activity, which may account for its efficacy in hypertension. Penerbit Universiti Kebangsaan Malaysia 2021-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18065/1/16.pdf Palla, Amber Hanif and Ur Rehman, Najeeb and Siddiqi, Hasan Salman and Gilani, Anwar-Ul-Hassan and Siddiqui, Bina Shaheen and Ilyas, Maimoona and Perwaiz, Sobiya (2021) Cardiac depressant and vasodialatory effect of flaxseed - basis for the medicinal use in hypertension. Sains Malaysiana, 50 (9). pp. 2687-2700. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil9_2021/KandunganJilid50Bil9_2021.html |
| spellingShingle | Palla, Amber Hanif Ur Rehman, Najeeb Siddiqi, Hasan Salman Gilani, Anwar-Ul-Hassan Siddiqui, Bina Shaheen Ilyas, Maimoona Perwaiz, Sobiya Cardiac depressant and vasodialatory effect of flaxseed - basis for the medicinal use in hypertension |
| title | Cardiac depressant and vasodialatory effect of flaxseed - basis for the medicinal use in hypertension |
| title_full | Cardiac depressant and vasodialatory effect of flaxseed - basis for the medicinal use in hypertension |
| title_fullStr | Cardiac depressant and vasodialatory effect of flaxseed - basis for the medicinal use in hypertension |
| title_full_unstemmed | Cardiac depressant and vasodialatory effect of flaxseed - basis for the medicinal use in hypertension |
| title_short | Cardiac depressant and vasodialatory effect of flaxseed - basis for the medicinal use in hypertension |
| title_sort | cardiac depressant and vasodialatory effect of flaxseed - basis for the medicinal use in hypertension |
| url | http://journalarticle.ukm.my/18065/ http://journalarticle.ukm.my/18065/ http://journalarticle.ukm.my/18065/1/16.pdf |