Impact of single and two-phase dissolved oxygen tension control on Bacillus thuringiensis cultivation and δ-endotoxin production
Constant δ-endotoxin production is crucial in Bacillus thuringiensis cultivation with aeration strategy playing a significant role in the process. In this study, the impact of DOT control strategies on the cultivation performance of B. thuringiensis was investigated using a 5-L stirred tank biorea...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17894/ http://journalarticle.ukm.my/17894/1/73-84%2B%2B%2BMohamed%2BMazmira%2BMohd%2BMasri.pdf |
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| author | Mohamed Mazmira Mohd Masri, Mohd Shawal Thakib Maidin, Arbakariya Ariff, |
| author_facet | Mohamed Mazmira Mohd Masri, Mohd Shawal Thakib Maidin, Arbakariya Ariff, |
| author_sort | Mohamed Mazmira Mohd Masri, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Constant δ-endotoxin production is crucial in Bacillus thuringiensis cultivation with aeration strategy playing a significant
role in the process. In this study, the impact of DOT control strategies on the cultivation performance of B. thuringiensis
was investigated using a 5-L stirred tank bioreactor. Single-phase DOT control recorded low spore count (<5.0 × 1011
spore/mL) and low percentage of sporulation (<45%). Two-stage DOT control cultivation with DOT at 80% during the
active growth phase, followed by a switch to 60% or 40% at mid-exponential growth phase contributed to enhanced
sporulation (>60%), high maximum cell count (1.5 cfu/mL) with δ-endotoxin formed as early as 8 hr of cultivation. A high
maximum specific growth rate, μmax (>0.1 h-1) was also essential in ensuring δ-endotoxin production. High cell count obtained
in cultivation with DOT level of 80% saturation during active growth was concurrent with high cell count, high μmax, fast
generation time, high OUR, high CER value, and high respiratory quotient (RQ) value. During the two-phase DOT control
cultivation, low RQ values were reported. The result showed that the exhaust gas data could be used to monitor B.
thuringiensis cultivation performance. |
| first_indexed | 2025-11-15T00:33:55Z |
| format | Article |
| id | oai:generic.eprints.org:17894 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:33:55Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:178942022-01-13T01:17:58Z http://journalarticle.ukm.my/17894/ Impact of single and two-phase dissolved oxygen tension control on Bacillus thuringiensis cultivation and δ-endotoxin production Mohamed Mazmira Mohd Masri, Mohd Shawal Thakib Maidin, Arbakariya Ariff, Constant δ-endotoxin production is crucial in Bacillus thuringiensis cultivation with aeration strategy playing a significant role in the process. In this study, the impact of DOT control strategies on the cultivation performance of B. thuringiensis was investigated using a 5-L stirred tank bioreactor. Single-phase DOT control recorded low spore count (<5.0 × 1011 spore/mL) and low percentage of sporulation (<45%). Two-stage DOT control cultivation with DOT at 80% during the active growth phase, followed by a switch to 60% or 40% at mid-exponential growth phase contributed to enhanced sporulation (>60%), high maximum cell count (1.5 cfu/mL) with δ-endotoxin formed as early as 8 hr of cultivation. A high maximum specific growth rate, μmax (>0.1 h-1) was also essential in ensuring δ-endotoxin production. High cell count obtained in cultivation with DOT level of 80% saturation during active growth was concurrent with high cell count, high μmax, fast generation time, high OUR, high CER value, and high respiratory quotient (RQ) value. During the two-phase DOT control cultivation, low RQ values were reported. The result showed that the exhaust gas data could be used to monitor B. thuringiensis cultivation performance. Penerbit Universiti Kebangsaan Malaysia 2021 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17894/1/73-84%2B%2B%2BMohamed%2BMazmira%2BMohd%2BMasri.pdf Mohamed Mazmira Mohd Masri, and Mohd Shawal Thakib Maidin, and Arbakariya Ariff, (2021) Impact of single and two-phase dissolved oxygen tension control on Bacillus thuringiensis cultivation and δ-endotoxin production. Malaysian Applied Biology, 50 (1). pp. 73-84. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/2 |
| spellingShingle | Mohamed Mazmira Mohd Masri, Mohd Shawal Thakib Maidin, Arbakariya Ariff, Impact of single and two-phase dissolved oxygen tension control on Bacillus thuringiensis cultivation and δ-endotoxin production |
| title | Impact of single and two-phase dissolved oxygen tension control on Bacillus thuringiensis cultivation and δ-endotoxin production |
| title_full | Impact of single and two-phase dissolved oxygen tension control on Bacillus thuringiensis cultivation and δ-endotoxin production |
| title_fullStr | Impact of single and two-phase dissolved oxygen tension control on Bacillus thuringiensis cultivation and δ-endotoxin production |
| title_full_unstemmed | Impact of single and two-phase dissolved oxygen tension control on Bacillus thuringiensis cultivation and δ-endotoxin production |
| title_short | Impact of single and two-phase dissolved oxygen tension control on Bacillus thuringiensis cultivation and δ-endotoxin production |
| title_sort | impact of single and two-phase dissolved oxygen tension control on bacillus thuringiensis cultivation and δ-endotoxin production |
| url | http://journalarticle.ukm.my/17894/ http://journalarticle.ukm.my/17894/ http://journalarticle.ukm.my/17894/1/73-84%2B%2B%2BMohamed%2BMazmira%2BMohd%2BMasri.pdf |