The combination of bFGF and hydrocortisone is a better alternative compared to 5-azacytidine for cardiomyogenic differentiation of bone marrow and adipose stem cells
Stem cells can be differentiated into cardiomyocytes by induction with 5-azacytidine (5-aza) but its carcinogenicity is of concern for future translational application. Alternatively, growth factors and hormones such as basic fibroblast growth factor (bFGF) and hydrocortisone have been reported to a...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17559/ http://journalarticle.ukm.my/17559/1/13.pdf |
| _version_ | 1848814343594967040 |
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| author | Nadiah Sulaiman, Nur Qisya Afifah Veronica Sainik, Shamsul Sulaiman, Pezhman Hafez, Ng, Min Hwei Ruszymah Hj Idrus, |
| author_facet | Nadiah Sulaiman, Nur Qisya Afifah Veronica Sainik, Shamsul Sulaiman, Pezhman Hafez, Ng, Min Hwei Ruszymah Hj Idrus, |
| author_sort | Nadiah Sulaiman, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Stem cells can be differentiated into cardiomyocytes by induction with 5-azacytidine (5-aza) but its carcinogenicity is of concern for future translational application. Alternatively, growth factors and hormones such as basic fibroblast growth factor (bFGF) and hydrocortisone have been reported to act as a therapeutic inducer for cardiomyocytes differentiation. In this study, we aim to investigate the ability of bFGF and hydrocortisone in combination to stimulate the differentiation of mesenchymal stem cells (MSC) into cardiomyocytes lineage. Sheep adipose tissue stem cell (ATSC) and bone marrow stem cell (BMSC) were isolated, cultured and induced with the three groups of induction factors; 5-aza alone, the combination of hydrocortisone and bFGF and all three factors in combination for cardiomyogenic differentiation. Morphological, protein and functional ability of both ATSC and BMSC were observed and analysed to confirm cardiomyocyte differentiation. Viability of BMSC and ATSC in each treated group was significantly higher (P < 0.05) on both cells after treated with 10 nM of bFGF and 50 μM of hydrocortisone. Cardiomyocyte proteins; α-Sarcomeric actin (αSA) and Phospolamban (Plb) was detected in both ATSC and BMSC exposed to induction factors but not in the control negative group. Both ATSC and BMSC without induction factors showed only minute cell number possesses αSA and Plb. Calcium ion (Ca2+) spark was observed in primary heart cells. Similarly, Ca2+ spark was also detected in induced ATSC and BMSC, proving some functionality of induced cells. In conclusion, bFGF and hydrocortisone are safer induction factor compared to the currently used 5-aza as both showed higher viability after induction, therefore more cells are available for future use in cardiac tissue engineering. |
| first_indexed | 2025-11-15T00:32:35Z |
| format | Article |
| id | oai:generic.eprints.org:17559 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:32:35Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:175592021-11-15T03:23:51Z http://journalarticle.ukm.my/17559/ The combination of bFGF and hydrocortisone is a better alternative compared to 5-azacytidine for cardiomyogenic differentiation of bone marrow and adipose stem cells Nadiah Sulaiman, Nur Qisya Afifah Veronica Sainik, Shamsul Sulaiman, Pezhman Hafez, Ng, Min Hwei Ruszymah Hj Idrus, Stem cells can be differentiated into cardiomyocytes by induction with 5-azacytidine (5-aza) but its carcinogenicity is of concern for future translational application. Alternatively, growth factors and hormones such as basic fibroblast growth factor (bFGF) and hydrocortisone have been reported to act as a therapeutic inducer for cardiomyocytes differentiation. In this study, we aim to investigate the ability of bFGF and hydrocortisone in combination to stimulate the differentiation of mesenchymal stem cells (MSC) into cardiomyocytes lineage. Sheep adipose tissue stem cell (ATSC) and bone marrow stem cell (BMSC) were isolated, cultured and induced with the three groups of induction factors; 5-aza alone, the combination of hydrocortisone and bFGF and all three factors in combination for cardiomyogenic differentiation. Morphological, protein and functional ability of both ATSC and BMSC were observed and analysed to confirm cardiomyocyte differentiation. Viability of BMSC and ATSC in each treated group was significantly higher (P < 0.05) on both cells after treated with 10 nM of bFGF and 50 μM of hydrocortisone. Cardiomyocyte proteins; α-Sarcomeric actin (αSA) and Phospolamban (Plb) was detected in both ATSC and BMSC exposed to induction factors but not in the control negative group. Both ATSC and BMSC without induction factors showed only minute cell number possesses αSA and Plb. Calcium ion (Ca2+) spark was observed in primary heart cells. Similarly, Ca2+ spark was also detected in induced ATSC and BMSC, proving some functionality of induced cells. In conclusion, bFGF and hydrocortisone are safer induction factor compared to the currently used 5-aza as both showed higher viability after induction, therefore more cells are available for future use in cardiac tissue engineering. Penerbit Universiti Kebangsaan Malaysia 2021-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17559/1/13.pdf Nadiah Sulaiman, and Nur Qisya Afifah Veronica Sainik, and Shamsul Sulaiman, and Pezhman Hafez, and Ng, Min Hwei and Ruszymah Hj Idrus, (2021) The combination of bFGF and hydrocortisone is a better alternative compared to 5-azacytidine for cardiomyogenic differentiation of bone marrow and adipose stem cells. Sains Malaysiana, 50 (7). pp. 1987-1996. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil7_2021/KandunganJilid50Bil7_2021.html |
| spellingShingle | Nadiah Sulaiman, Nur Qisya Afifah Veronica Sainik, Shamsul Sulaiman, Pezhman Hafez, Ng, Min Hwei Ruszymah Hj Idrus, The combination of bFGF and hydrocortisone is a better alternative compared to 5-azacytidine for cardiomyogenic differentiation of bone marrow and adipose stem cells |
| title | The combination of bFGF and hydrocortisone is a better alternative compared to 5-azacytidine for cardiomyogenic differentiation of bone marrow and adipose stem cells |
| title_full | The combination of bFGF and hydrocortisone is a better alternative compared to 5-azacytidine for cardiomyogenic differentiation of bone marrow and adipose stem cells |
| title_fullStr | The combination of bFGF and hydrocortisone is a better alternative compared to 5-azacytidine for cardiomyogenic differentiation of bone marrow and adipose stem cells |
| title_full_unstemmed | The combination of bFGF and hydrocortisone is a better alternative compared to 5-azacytidine for cardiomyogenic differentiation of bone marrow and adipose stem cells |
| title_short | The combination of bFGF and hydrocortisone is a better alternative compared to 5-azacytidine for cardiomyogenic differentiation of bone marrow and adipose stem cells |
| title_sort | combination of bfgf and hydrocortisone is a better alternative compared to 5-azacytidine for cardiomyogenic differentiation of bone marrow and adipose stem cells |
| url | http://journalarticle.ukm.my/17559/ http://journalarticle.ukm.my/17559/ http://journalarticle.ukm.my/17559/1/13.pdf |