Production of docosahexaenoic acid, DHA using different modes of cultivation by Aurantiochytrium sp. SW1
Thraustochytrids, such as members of the genus Aurantiochytrium, are rich in docosahexaenoic acid (DHA, C22:6n-3) and represent a promising source of omega-3 fatty acids which plays a vital role in the enhancement of human health, particularly for neurological and visual functions. Different modes o...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17556/ http://journalarticle.ukm.my/17556/1/10.pdf |
| _version_ | 1848814342723600384 |
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| author | Shariffah Nurhidayah Syed Abdul Rahman, Mohd Sahaid Kalil, Aidil Abdul Hamid, |
| author_facet | Shariffah Nurhidayah Syed Abdul Rahman, Mohd Sahaid Kalil, Aidil Abdul Hamid, |
| author_sort | Shariffah Nurhidayah Syed Abdul Rahman, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Thraustochytrids, such as members of the genus Aurantiochytrium, are rich in docosahexaenoic acid (DHA, C22:6n-3) and represent a promising source of omega-3 fatty acids which plays a vital role in the enhancement of human health, particularly for neurological and visual functions. Different modes of cultivation (batch, fed-batch and repeated-batch) by Aurantiochytrium sp. SW1 were studied for effective docosahexaenoic acid (DHA) production. In this study, three different modes of fermentation were carried out in 1 L shake flasks with a working volume of 500 mL, incubated at 30 ºC and 200 rpm. Batch cultivation significantly exceeds the rest of cultivation modes, achieving maximal lipid and DHA concentrations of 11.22 g/L and 5.87 g/L, respectively, and DHA productivity of 0.061 g/L/h. Lipid and DHA concentration of the repeated-batch process decreased through the cycles for all three different types of replacement ratio (80, 90 and 95%). The average decrease percentage of DHA concentration for cycle one and cycle two were 21.76 and 32.52%, respectively. However, the fatty acid composition of lipids obtained in the cycles remained consistent with 16:0 and DHA being the most abundant fatty acids indicating that this mode of fermentation is highly useable for industrial applications. |
| first_indexed | 2025-11-15T00:32:34Z |
| format | Article |
| id | oai:generic.eprints.org:17556 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:32:34Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:175562021-11-15T01:57:43Z http://journalarticle.ukm.my/17556/ Production of docosahexaenoic acid, DHA using different modes of cultivation by Aurantiochytrium sp. SW1 Shariffah Nurhidayah Syed Abdul Rahman, Mohd Sahaid Kalil, Aidil Abdul Hamid, Thraustochytrids, such as members of the genus Aurantiochytrium, are rich in docosahexaenoic acid (DHA, C22:6n-3) and represent a promising source of omega-3 fatty acids which plays a vital role in the enhancement of human health, particularly for neurological and visual functions. Different modes of cultivation (batch, fed-batch and repeated-batch) by Aurantiochytrium sp. SW1 were studied for effective docosahexaenoic acid (DHA) production. In this study, three different modes of fermentation were carried out in 1 L shake flasks with a working volume of 500 mL, incubated at 30 ºC and 200 rpm. Batch cultivation significantly exceeds the rest of cultivation modes, achieving maximal lipid and DHA concentrations of 11.22 g/L and 5.87 g/L, respectively, and DHA productivity of 0.061 g/L/h. Lipid and DHA concentration of the repeated-batch process decreased through the cycles for all three different types of replacement ratio (80, 90 and 95%). The average decrease percentage of DHA concentration for cycle one and cycle two were 21.76 and 32.52%, respectively. However, the fatty acid composition of lipids obtained in the cycles remained consistent with 16:0 and DHA being the most abundant fatty acids indicating that this mode of fermentation is highly useable for industrial applications. Penerbit Universiti Kebangsaan Malaysia 2021-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17556/1/10.pdf Shariffah Nurhidayah Syed Abdul Rahman, and Mohd Sahaid Kalil, and Aidil Abdul Hamid, (2021) Production of docosahexaenoic acid, DHA using different modes of cultivation by Aurantiochytrium sp. SW1. Sains Malaysiana, 50 (7). pp. 1947-1957. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil7_2021/KandunganJilid50Bil7_2021.html |
| spellingShingle | Shariffah Nurhidayah Syed Abdul Rahman, Mohd Sahaid Kalil, Aidil Abdul Hamid, Production of docosahexaenoic acid, DHA using different modes of cultivation by Aurantiochytrium sp. SW1 |
| title | Production of docosahexaenoic acid, DHA using different modes of cultivation by Aurantiochytrium sp. SW1 |
| title_full | Production of docosahexaenoic acid, DHA using different modes of cultivation by Aurantiochytrium sp. SW1 |
| title_fullStr | Production of docosahexaenoic acid, DHA using different modes of cultivation by Aurantiochytrium sp. SW1 |
| title_full_unstemmed | Production of docosahexaenoic acid, DHA using different modes of cultivation by Aurantiochytrium sp. SW1 |
| title_short | Production of docosahexaenoic acid, DHA using different modes of cultivation by Aurantiochytrium sp. SW1 |
| title_sort | production of docosahexaenoic acid, dha using different modes of cultivation by aurantiochytrium sp. sw1 |
| url | http://journalarticle.ukm.my/17556/ http://journalarticle.ukm.my/17556/ http://journalarticle.ukm.my/17556/1/10.pdf |