Physicochemical properties and glucose-lowering effect of an insulin - Aloe vera buccal delivery system

Subcutaneous insulin injection is one of the therapies in the treatment of diabetes mellitus. However, problems such as pain at the injection site and lipodystrophy present a challenge that will influence patient compliance. This study aims to develop and characterise buccal film formulations cont...

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Main Authors: Chin, Heng Huay, Rehman, Khurram, Ng, Pei-Yuen, Mohd Hanif Zulfakar
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Online Access:http://journalarticle.ukm.my/17539/
http://journalarticle.ukm.my/17539/1/21.pdf
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author Chin, Heng Huay
Rehman, Khurram
Ng, Pei-Yuen
Mohd Hanif Zulfakar,
author_facet Chin, Heng Huay
Rehman, Khurram
Ng, Pei-Yuen
Mohd Hanif Zulfakar,
author_sort Chin, Heng Huay
building UKM Institutional Repository
collection Online Access
description Subcutaneous insulin injection is one of the therapies in the treatment of diabetes mellitus. However, problems such as pain at the injection site and lipodystrophy present a challenge that will influence patient compliance. This study aims to develop and characterise buccal film formulations containing insulin, utilising the glucose-lowering and pharmaceutical properties of Aloe vera. Characterisation tests such as morphology, rheological measurement, pH value, mechanical properties, and permeation test were performed on the optimal formulation. Assessment of the glucose-lowering efficacy was performed using alloxan-induced diabetic rabbits. Composition of the final film formulation included 3% w/w sodium carboxymethyl cellulose, 40% v/v glycerol, 70% v/v Aloe vera, 0.5% w/v mannitol, 0.125% w/v aspartame, 0.125% v/v Tween 80 and 15.3 mg insulin. The drug content has been determined to be 56.77 ± 8.55%. The formulation shows a low variation in weight and thickness measurements and acceptable physicochemical properties, in addition to sustained drug release for 6 h. The final film formulation that combined insulin and Aloe vera was effective enough to reduce blood glucose levels compared to the negative control (p > 0.05), despite fluctuation in the blood glucose levels throughout the study, and did not show any enhancement in reduction compared to the insulin-only film. In conclusion, the developed buccal film formulation has the potential to be used as a vessel for the delivery of insulin. Nevertheless, further studies are required to ensure the stability of the insulin via buccal route and stabilisation of insulin within the formulation.
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spelling oai:generic.eprints.org:175392021-10-26T06:23:16Z http://journalarticle.ukm.my/17539/ Physicochemical properties and glucose-lowering effect of an insulin - Aloe vera buccal delivery system Chin, Heng Huay Rehman, Khurram Ng, Pei-Yuen Mohd Hanif Zulfakar, Subcutaneous insulin injection is one of the therapies in the treatment of diabetes mellitus. However, problems such as pain at the injection site and lipodystrophy present a challenge that will influence patient compliance. This study aims to develop and characterise buccal film formulations containing insulin, utilising the glucose-lowering and pharmaceutical properties of Aloe vera. Characterisation tests such as morphology, rheological measurement, pH value, mechanical properties, and permeation test were performed on the optimal formulation. Assessment of the glucose-lowering efficacy was performed using alloxan-induced diabetic rabbits. Composition of the final film formulation included 3% w/w sodium carboxymethyl cellulose, 40% v/v glycerol, 70% v/v Aloe vera, 0.5% w/v mannitol, 0.125% w/v aspartame, 0.125% v/v Tween 80 and 15.3 mg insulin. The drug content has been determined to be 56.77 ± 8.55%. The formulation shows a low variation in weight and thickness measurements and acceptable physicochemical properties, in addition to sustained drug release for 6 h. The final film formulation that combined insulin and Aloe vera was effective enough to reduce blood glucose levels compared to the negative control (p > 0.05), despite fluctuation in the blood glucose levels throughout the study, and did not show any enhancement in reduction compared to the insulin-only film. In conclusion, the developed buccal film formulation has the potential to be used as a vessel for the delivery of insulin. Nevertheless, further studies are required to ensure the stability of the insulin via buccal route and stabilisation of insulin within the formulation. Penerbit Universiti Kebangsaan Malaysia 2021-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17539/1/21.pdf Chin, Heng Huay and Rehman, Khurram and Ng, Pei-Yuen and Mohd Hanif Zulfakar, (2021) Physicochemical properties and glucose-lowering effect of an insulin - Aloe vera buccal delivery system. Sains Malaysiana, 50 (6). pp. 1755-1765. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil6_2021/KandunganJilid50Bil6_2021.html
spellingShingle Chin, Heng Huay
Rehman, Khurram
Ng, Pei-Yuen
Mohd Hanif Zulfakar,
Physicochemical properties and glucose-lowering effect of an insulin - Aloe vera buccal delivery system
title Physicochemical properties and glucose-lowering effect of an insulin - Aloe vera buccal delivery system
title_full Physicochemical properties and glucose-lowering effect of an insulin - Aloe vera buccal delivery system
title_fullStr Physicochemical properties and glucose-lowering effect of an insulin - Aloe vera buccal delivery system
title_full_unstemmed Physicochemical properties and glucose-lowering effect of an insulin - Aloe vera buccal delivery system
title_short Physicochemical properties and glucose-lowering effect of an insulin - Aloe vera buccal delivery system
title_sort physicochemical properties and glucose-lowering effect of an insulin - aloe vera buccal delivery system
url http://journalarticle.ukm.my/17539/
http://journalarticle.ukm.my/17539/
http://journalarticle.ukm.my/17539/1/21.pdf