Physicochemical properties and glucose-lowering effect of an insulin - Aloe vera buccal delivery system
Subcutaneous insulin injection is one of the therapies in the treatment of diabetes mellitus. However, problems such as pain at the injection site and lipodystrophy present a challenge that will influence patient compliance. This study aims to develop and characterise buccal film formulations cont...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17539/ http://journalarticle.ukm.my/17539/1/21.pdf |
| Summary: | Subcutaneous insulin injection is one of the therapies in the treatment of diabetes mellitus. However, problems such as
pain at the injection site and lipodystrophy present a challenge that will influence patient compliance. This study aims to
develop and characterise buccal film formulations containing insulin, utilising the glucose-lowering and pharmaceutical
properties of Aloe vera. Characterisation tests such as morphology, rheological measurement, pH value, mechanical
properties, and permeation test were performed on the optimal formulation. Assessment of the glucose-lowering efficacy
was performed using alloxan-induced diabetic rabbits. Composition of the final film formulation included 3% w/w sodium
carboxymethyl cellulose, 40% v/v glycerol, 70% v/v Aloe vera, 0.5% w/v mannitol, 0.125% w/v aspartame, 0.125% v/v
Tween 80 and 15.3 mg insulin. The drug content has been determined to be 56.77 ± 8.55%. The formulation shows a low
variation in weight and thickness measurements and acceptable physicochemical properties, in addition to sustained
drug release for 6 h. The final film formulation that combined insulin and Aloe vera was effective enough to reduce blood
glucose levels compared to the negative control (p > 0.05), despite fluctuation in the blood glucose levels throughout the
study, and did not show any enhancement in reduction compared to the insulin-only film. In conclusion, the developed
buccal film formulation has the potential to be used as a vessel for the delivery of insulin. Nevertheless, further studies
are required to ensure the stability of the insulin via buccal route and stabilisation of insulin within the formulation. |
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