Immunomodulatory properties of wharton’s jelly-derived mesenchymal stem cells from three anatomical segments of umbilical cord
Mesenchymal stem cells (MSCs) are multipotent progenitor cells that are reported to be immune-privileged and immuneevasive. MSCs are capable of differentiating into specific cell types for subsequent use in cell-based therapy. They express low levels of human leucocyte antigen (HLA)-ABC and no HLA...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17536/ http://journalarticle.ukm.my/17536/1/18.pdf |
| Summary: | Mesenchymal stem cells (MSCs) are multipotent progenitor cells that are reported to be immune-privileged and immuneevasive.
MSCs are capable of differentiating into specific cell types for subsequent use in cell-based therapy. They
express low levels of human leucocyte antigen (HLA)-ABC and no HLA-DR. Wharton’s jelly-derived MSCs (WJ-MSCs)
were also found to express human leukocyte antigen G (HLA-G), which renders them immunosuppressive. This study
aimed to determine whether cultured WJ-MSCs retain their immune-privileged and immune-evasive properties after
cell differentiation, and whether these properties differ among MSCs derived from different anatomical segments of the
umbilical cord. Umbilical cords of healthy pregnant mothers undergoing caesarean section were obtained and grouped
by three anatomical segments: fetal, middle, and maternal segments. WJ-MSCs were isolated, culture-expanded, and
differentiated into osteogenic cells. Expression of HLA-DR, HLA-ABC, and HLA-G were quantified using flow cytometry.
Both undifferentiated and osteodifferentiated WJ-MSCs were subsequently co-cultured with allogeneic peripheral blood
mononuclear cells with/without lipopolysaccharide (LPS) stimulation for five days. Lymphocyte proliferation assay was
performed using carboxyfluorescein succinimidyl ester (CFSE) as a tracker. Our results showed no significant difference
existed in the HLA profiles among WJ-MSCs from different segments and between WJ-MSCs with and without osteogenic
differentiation. Mean levels for HLA-G, HLABC, and HLA-DR were 24.82±17.64, 52.50±18.41, and 1.00±1.68%,
respectively. Stimulation with LPS and WJ-MSCs increased peripheral blooc mononuclear cells (PBMC) proliferation.
However, PBMC proliferation was significantly lower when PBMCs were co-cultured with osteodifferentiated WJ-MSCs (p
< .05; with LPS stimulation and p < .001 without LPS stimulation) than when they were co-cultured with undifferentiated
WJ-MSCs. These findings suggest that cultured WJ-MSCs stimulate lymphocyte proliferation and are not immune-privileged.
Osteodifferentiated WJ-MSCs reduced the immunogenicity of WJ-MSCs, and this reduction in PBMC proliferation was
even more pronounced in the presence of LPS (p < .05). In conclusion, cultured WJ-MSCs are not immune-privileged.
Osteodifferentiated WJ-MSCs are less immunogenic than undifferentiated WJ-MSCs, in which case hypoimmunogenicity
is more profound under LPS-stimulated conditions. |
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