Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice
Extended dermal exposure of ultraviolet B (UVB) can induce erythema, hyperpigmentation, epidermal hyperplasia and cancer. Natural active compound such as pterostilbene (PS) is a potential UV-protecting agent as it has broad biological activities. This study aimed to evaluate the photoprotective...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17416/ http://journalarticle.ukm.my/17416/1/19.pdf |
| _version_ | 1848814309232082944 |
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| author | Tava Shelan Nagapan, Lim, Wenna Nallance Ahmad Rohi Ghazali, Dayang Fredalina Basri, |
| author_facet | Tava Shelan Nagapan, Lim, Wenna Nallance Ahmad Rohi Ghazali, Dayang Fredalina Basri, |
| author_sort | Tava Shelan Nagapan, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Extended dermal exposure of ultraviolet B (UVB) can induce erythema, hyperpigmentation, epidermal hyperplasia and
cancer. Natural active compound such as pterostilbene (PS) is a potential UV-protecting agent as it has broad biological
activities. This study aimed to evaluate the photoprotective effect of pterostilbene on ultraviolet-B-induced BALB/c
mice. Twenty-four female mice were randomised into four groups (n=6/group): vehicle control (VC); UVB irradiated
only (UVB only); UVB irradiation treated with pterostilbene 10 mg/kg (PS10+UVB); (iv) UVB irradiation treated with
pterostilbene 20 mg/kg (PS20+UVB). The PS treatments were given for 14 days, and UVB was given at dose 250 mJ/
cm2
on days 9, 11, and 13 of the treatment periods. The results showed that PS lessened redness and scaling on the
skin of UVB-irradiated mice. The skinfold thickness and epidermal thickness in the PS-treated group were significantly
reduced (p<0.05) in comparison with those in the UVB only group. The PS10 and PS20 groups (5.927 ± 0.354 and
5.660 ± 0.765 nmol/g, respectively) demonstrated significantly decreased MDA levels (P<0.05) relative to the UVB
only group (13.343 ± 1.350 nmol/g). The GSH level in both PS10 (0.555 ± 0.020 µmol/mg) and PS20 (0.568 ± 0.055
µmol/mg) groups increased significantly (p<0.05) compared with that in the UVB only group (0.376 ± 0.025 µmol/mg).
SOD activity in the PS20 group (1.388 ± 0.172 U/min/mg) increased significantly (p<0.05) compared with that in
the UVB only group (0.561 ± 0.034 U/min/mg). Histological observation showed that PS reduced leukocyte infiltration
and epidermal hyperplasia. Hence, oral PS may exert a photoprotective effect by acting as an anti-inflammatory and
antioxidant agent on UVB-irradiated mice skin. |
| first_indexed | 2025-11-15T00:32:02Z |
| format | Article |
| id | oai:generic.eprints.org:17416 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:32:02Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:174162021-09-14T03:08:15Z http://journalarticle.ukm.my/17416/ Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice Tava Shelan Nagapan, Lim, Wenna Nallance Ahmad Rohi Ghazali, Dayang Fredalina Basri, Extended dermal exposure of ultraviolet B (UVB) can induce erythema, hyperpigmentation, epidermal hyperplasia and cancer. Natural active compound such as pterostilbene (PS) is a potential UV-protecting agent as it has broad biological activities. This study aimed to evaluate the photoprotective effect of pterostilbene on ultraviolet-B-induced BALB/c mice. Twenty-four female mice were randomised into four groups (n=6/group): vehicle control (VC); UVB irradiated only (UVB only); UVB irradiation treated with pterostilbene 10 mg/kg (PS10+UVB); (iv) UVB irradiation treated with pterostilbene 20 mg/kg (PS20+UVB). The PS treatments were given for 14 days, and UVB was given at dose 250 mJ/ cm2 on days 9, 11, and 13 of the treatment periods. The results showed that PS lessened redness and scaling on the skin of UVB-irradiated mice. The skinfold thickness and epidermal thickness in the PS-treated group were significantly reduced (p<0.05) in comparison with those in the UVB only group. The PS10 and PS20 groups (5.927 ± 0.354 and 5.660 ± 0.765 nmol/g, respectively) demonstrated significantly decreased MDA levels (P<0.05) relative to the UVB only group (13.343 ± 1.350 nmol/g). The GSH level in both PS10 (0.555 ± 0.020 µmol/mg) and PS20 (0.568 ± 0.055 µmol/mg) groups increased significantly (p<0.05) compared with that in the UVB only group (0.376 ± 0.025 µmol/mg). SOD activity in the PS20 group (1.388 ± 0.172 U/min/mg) increased significantly (p<0.05) compared with that in the UVB only group (0.561 ± 0.034 U/min/mg). Histological observation showed that PS reduced leukocyte infiltration and epidermal hyperplasia. Hence, oral PS may exert a photoprotective effect by acting as an anti-inflammatory and antioxidant agent on UVB-irradiated mice skin. Penerbit Universiti Kebangsaan Malaysia 2021-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17416/1/19.pdf Tava Shelan Nagapan, and Lim, Wenna Nallance and Ahmad Rohi Ghazali, and Dayang Fredalina Basri, (2021) Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice. Sains Malaysiana, 50 (5). pp. 1407-1414. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil5_2021/KandunganJilid50Bil5_2021.html |
| spellingShingle | Tava Shelan Nagapan, Lim, Wenna Nallance Ahmad Rohi Ghazali, Dayang Fredalina Basri, Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
| title | Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
| title_full | Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
| title_fullStr | Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
| title_full_unstemmed | Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
| title_short | Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
| title_sort | pterostilbene supplementation inhibits early inflammatory response and oxidative stress in uvb-induced balb/c mice |
| url | http://journalarticle.ukm.my/17416/ http://journalarticle.ukm.my/17416/ http://journalarticle.ukm.my/17416/1/19.pdf |