Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples
A new analytical method based on vortex-assisted liquid-liquid microextraction with back extraction (VALLME-BE) coupled with high performance liquid chromatography was developed for the simultaneous determination of antidiabetic drugs; repaglinide, glibenclamide, and glimepiride in water samples....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17407/ http://journalarticle.ukm.my/17407/1/10.pdf |
| _version_ | 1848814306471182336 |
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| author | Sohaib Jumaah Owaid Luhaib, Noorfatimah Yahaya, Alshishani, Anas Maizatul Najwa Jajuli, Mazidatulakmam Miskam, |
| author_facet | Sohaib Jumaah Owaid Luhaib, Noorfatimah Yahaya, Alshishani, Anas Maizatul Najwa Jajuli, Mazidatulakmam Miskam, |
| author_sort | Sohaib Jumaah Owaid Luhaib, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | A new analytical method based on vortex-assisted liquid-liquid microextraction with back extraction (VALLME-BE)
coupled with high performance liquid chromatography was developed for the simultaneous determination of antidiabetic
drugs; repaglinide, glibenclamide, and glimepiride in water samples. Chromatographic separation was achieved using
C18 column (250 × 4.6 mm × 5 µm) and methanol-phosphate buffer (pH3.7) in the ratio of 70:30 v/v as a mobile phase
at a flow rate of 1 mLmin-1. VALLME-BE was performed using 200 μL of n-octane dispersed into the aqueous sample
(10 mL) with the aid of vortexing agitation. Then, the analytes were back-extracted from the organic solvent to 0.05
M NaOH (40 µL). Under these conditions, enrichment factor of 155-fold was achieved. The developed VALLME-BE
method showed excellent linearity in the range of 30 to 1000 µgL-1 with limit of detection (LOD) of 0.41-1.66 µgL-1 and
limit of quantification (LOQ) of 1.38-5.54. 41-1.66 µgL-1. VALLME-BE was applied for the determination of repaglinide,
glibenclamide and glimepiride in water samples with the recoveries ranged from 83-109%. The relative standard deviation
for inter-day and intra-day precision was less than 9.9%. |
| first_indexed | 2025-11-15T00:31:59Z |
| format | Article |
| id | oai:generic.eprints.org:17407 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:31:59Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:174072021-09-14T02:50:23Z http://journalarticle.ukm.my/17407/ Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples Sohaib Jumaah Owaid Luhaib, Noorfatimah Yahaya, Alshishani, Anas Maizatul Najwa Jajuli, Mazidatulakmam Miskam, A new analytical method based on vortex-assisted liquid-liquid microextraction with back extraction (VALLME-BE) coupled with high performance liquid chromatography was developed for the simultaneous determination of antidiabetic drugs; repaglinide, glibenclamide, and glimepiride in water samples. Chromatographic separation was achieved using C18 column (250 × 4.6 mm × 5 µm) and methanol-phosphate buffer (pH3.7) in the ratio of 70:30 v/v as a mobile phase at a flow rate of 1 mLmin-1. VALLME-BE was performed using 200 μL of n-octane dispersed into the aqueous sample (10 mL) with the aid of vortexing agitation. Then, the analytes were back-extracted from the organic solvent to 0.05 M NaOH (40 µL). Under these conditions, enrichment factor of 155-fold was achieved. The developed VALLME-BE method showed excellent linearity in the range of 30 to 1000 µgL-1 with limit of detection (LOD) of 0.41-1.66 µgL-1 and limit of quantification (LOQ) of 1.38-5.54. 41-1.66 µgL-1. VALLME-BE was applied for the determination of repaglinide, glibenclamide and glimepiride in water samples with the recoveries ranged from 83-109%. The relative standard deviation for inter-day and intra-day precision was less than 9.9%. Penerbit Universiti Kebangsaan Malaysia 2021-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17407/1/10.pdf Sohaib Jumaah Owaid Luhaib, and Noorfatimah Yahaya, and Alshishani, Anas and Maizatul Najwa Jajuli, and Mazidatulakmam Miskam, (2021) Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples. Sains Malaysiana, 50 (5). pp. 1297-1307. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil5_2021/KandunganJilid50Bil5_2021.html |
| spellingShingle | Sohaib Jumaah Owaid Luhaib, Noorfatimah Yahaya, Alshishani, Anas Maizatul Najwa Jajuli, Mazidatulakmam Miskam, Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples |
| title | Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples |
| title_full | Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples |
| title_fullStr | Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples |
| title_full_unstemmed | Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples |
| title_short | Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples |
| title_sort | vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples |
| url | http://journalarticle.ukm.my/17407/ http://journalarticle.ukm.my/17407/ http://journalarticle.ukm.my/17407/1/10.pdf |