The effect of MS media strength and cytokinin in the induction of shoots from shoot tip explants of Australian Finger Lime (Citrus australasica cv. Tasty Green)
The Australian Finger Lime (Citrus australasica) is a type of citrus from the Rutaceae family, endemic to the east coast of Australia. The finger lime, loaded with numerous vitamins and renders a unique taste, has also been backed by science to contain essential amounts of antioxidants that are...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17405/ http://journalarticle.ukm.my/17405/1/8.pdf |
| _version_ | 1848814305929068544 |
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| author | Dahmendra Sriskanda, Poi, Khoy Siong Najwa Amalina Haradzi, Nurulhikma Md Isa, Sreeramanan Subramaniam, Chew, Bee Lynn |
| author_facet | Dahmendra Sriskanda, Poi, Khoy Siong Najwa Amalina Haradzi, Nurulhikma Md Isa, Sreeramanan Subramaniam, Chew, Bee Lynn |
| author_sort | Dahmendra Sriskanda, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The Australian Finger Lime (Citrus australasica) is a type of citrus from the Rutaceae family, endemic to the east coast of
Australia. The finger lime, loaded with numerous vitamins and renders a unique taste, has also been backed by science
to contain essential amounts of antioxidants that are beneficial for cell protection, immune response, cancer prevention,
ageing, arthritis and prevention of kidney stones. Current propagation attempts still rely on conventional methods that
are less efficient and resulted in the slow establishment of farms for finger lime especially for commercialization purposes.
This study focuses on the induction of shoots from shoot tip explants using 6-Benzylaminopurine (BAP) and Kinetin.
Aseptic explants were inoculated into Murashige and Skoog (MS) medium of full-strength and half-strength followed by
full-strength MS media supplemented with different concentrations of BAP and Kinetin. Results obtained in this study
showed no significant differences in terms of the number of axillary shoots produced between explants cultured in full
and half-strength MS media. However, the highest number of shoots and increment in shoot length were obtained from
MS media supplemented with 2.0 mg/L BAP with the values 1.80 ± 0.27 and 2.56 ± 0.36 cm, respectively. In conclusion,
MS media supplemented with 2.0 mg/L BAP was found optimal in the induction of shoots and shoot elongation of C.
australasica cv. Tasty Green. |
| first_indexed | 2025-11-15T00:31:59Z |
| format | Article |
| id | oai:generic.eprints.org:17405 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:31:59Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:174052021-09-14T02:23:26Z http://journalarticle.ukm.my/17405/ The effect of MS media strength and cytokinin in the induction of shoots from shoot tip explants of Australian Finger Lime (Citrus australasica cv. Tasty Green) Dahmendra Sriskanda, Poi, Khoy Siong Najwa Amalina Haradzi, Nurulhikma Md Isa, Sreeramanan Subramaniam, Chew, Bee Lynn The Australian Finger Lime (Citrus australasica) is a type of citrus from the Rutaceae family, endemic to the east coast of Australia. The finger lime, loaded with numerous vitamins and renders a unique taste, has also been backed by science to contain essential amounts of antioxidants that are beneficial for cell protection, immune response, cancer prevention, ageing, arthritis and prevention of kidney stones. Current propagation attempts still rely on conventional methods that are less efficient and resulted in the slow establishment of farms for finger lime especially for commercialization purposes. This study focuses on the induction of shoots from shoot tip explants using 6-Benzylaminopurine (BAP) and Kinetin. Aseptic explants were inoculated into Murashige and Skoog (MS) medium of full-strength and half-strength followed by full-strength MS media supplemented with different concentrations of BAP and Kinetin. Results obtained in this study showed no significant differences in terms of the number of axillary shoots produced between explants cultured in full and half-strength MS media. However, the highest number of shoots and increment in shoot length were obtained from MS media supplemented with 2.0 mg/L BAP with the values 1.80 ± 0.27 and 2.56 ± 0.36 cm, respectively. In conclusion, MS media supplemented with 2.0 mg/L BAP was found optimal in the induction of shoots and shoot elongation of C. australasica cv. Tasty Green. Penerbit Universiti Kebangsaan Malaysia 2021-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17405/1/8.pdf Dahmendra Sriskanda, and Poi, Khoy Siong and Najwa Amalina Haradzi, and Nurulhikma Md Isa, and Sreeramanan Subramaniam, and Chew, Bee Lynn (2021) The effect of MS media strength and cytokinin in the induction of shoots from shoot tip explants of Australian Finger Lime (Citrus australasica cv. Tasty Green). Sains Malaysiana, 50 (5). pp. 1277-1284. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil5_2021/KandunganJilid50Bil5_2021.html |
| spellingShingle | Dahmendra Sriskanda, Poi, Khoy Siong Najwa Amalina Haradzi, Nurulhikma Md Isa, Sreeramanan Subramaniam, Chew, Bee Lynn The effect of MS media strength and cytokinin in the induction of shoots from shoot tip explants of Australian Finger Lime (Citrus australasica cv. Tasty Green) |
| title | The effect of MS media strength and cytokinin in the induction of shoots from shoot tip explants of Australian Finger Lime (Citrus australasica cv. Tasty Green) |
| title_full | The effect of MS media strength and cytokinin in the induction of shoots from shoot tip explants of Australian Finger Lime (Citrus australasica cv. Tasty Green) |
| title_fullStr | The effect of MS media strength and cytokinin in the induction of shoots from shoot tip explants of Australian Finger Lime (Citrus australasica cv. Tasty Green) |
| title_full_unstemmed | The effect of MS media strength and cytokinin in the induction of shoots from shoot tip explants of Australian Finger Lime (Citrus australasica cv. Tasty Green) |
| title_short | The effect of MS media strength and cytokinin in the induction of shoots from shoot tip explants of Australian Finger Lime (Citrus australasica cv. Tasty Green) |
| title_sort | effect of ms media strength and cytokinin in the induction of shoots from shoot tip explants of australian finger lime (citrus australasica cv. tasty green) |
| url | http://journalarticle.ukm.my/17405/ http://journalarticle.ukm.my/17405/ http://journalarticle.ukm.my/17405/1/8.pdf |