Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract
Pomacea sp. is a freshwater gastropod that is capable of withstanding oxidative stress during extreme environmental changes. The snail enzymatic oxidative responses have already been elucidated through biochemical, transcriptomics, and proteomics analysis. However, their non-enzymatic oxidative re...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/17390/ http://journalarticle.ukm.my/17390/1/49_05_12.pdf |
| _version_ | 1848814303552995328 |
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| author | Khalida Khalil, Syarul Nataqain Baharum, Shazrul Fazry, Nik Marzuki Sidik, Fareed Sairi, |
| author_facet | Khalida Khalil, Syarul Nataqain Baharum, Shazrul Fazry, Nik Marzuki Sidik, Fareed Sairi, |
| author_sort | Khalida Khalil, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Pomacea sp. is a freshwater gastropod that is capable of withstanding oxidative stress during extreme environmental changes.
The snail enzymatic oxidative responses have already been elucidated through biochemical, transcriptomics, and proteomics
analysis. However, their non-enzymatic oxidative responses have yet to be elucidated. Therefore, this study aims to characterize
the antioxidant activity and identify the non-enzymatic antioxidant compounds from Pomacea maculata. To address the aims,
a polar and non-polar extraction of snail-whole body extract was conducted using methanol and chloroform, respectively.
The antioxidant activity of both extracts was elucidated by Folin Ciocalteau (FC), DPPH, and reducing power assay. LC-MS/MS was then used to profile both extracts. The results demonstrated that the crude methanol extract (CME) contains a
higher antioxidant capacity (FC=43.22 ± 3.02 mg GAE/ g extract, DPPH IC50=0.073 mg/mL, and reducing the power of
methanol and chloroform extract are 0.361 ± 0.07 and 0.051 ± 0.003 respectively). Profiling of the snail metabolites by LC-MS/MS from both extracts resulted in the identification of uric acid and phenolic compounds . The former was detected at
the highest intensity in CME followed by crude chloroform extract (CCE). The phenolic compounds, however, were
hypothetically identified as plant metabolites. Therefore, the study suggested that antioxidant activity exhibited by P. maculata
extracts were due to non-enzymatic compounds such as uric acid and phenolic compounds originated from the animal’s
metabolic activity and plants, respectively. |
| first_indexed | 2025-11-15T00:31:57Z |
| format | Article |
| id | oai:generic.eprints.org:17390 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:31:57Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:173902021-08-30T03:59:06Z http://journalarticle.ukm.my/17390/ Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract Khalida Khalil, Syarul Nataqain Baharum, Shazrul Fazry, Nik Marzuki Sidik, Fareed Sairi, Pomacea sp. is a freshwater gastropod that is capable of withstanding oxidative stress during extreme environmental changes. The snail enzymatic oxidative responses have already been elucidated through biochemical, transcriptomics, and proteomics analysis. However, their non-enzymatic oxidative responses have yet to be elucidated. Therefore, this study aims to characterize the antioxidant activity and identify the non-enzymatic antioxidant compounds from Pomacea maculata. To address the aims, a polar and non-polar extraction of snail-whole body extract was conducted using methanol and chloroform, respectively. The antioxidant activity of both extracts was elucidated by Folin Ciocalteau (FC), DPPH, and reducing power assay. LC-MS/MS was then used to profile both extracts. The results demonstrated that the crude methanol extract (CME) contains a higher antioxidant capacity (FC=43.22 ± 3.02 mg GAE/ g extract, DPPH IC50=0.073 mg/mL, and reducing the power of methanol and chloroform extract are 0.361 ± 0.07 and 0.051 ± 0.003 respectively). Profiling of the snail metabolites by LC-MS/MS from both extracts resulted in the identification of uric acid and phenolic compounds . The former was detected at the highest intensity in CME followed by crude chloroform extract (CCE). The phenolic compounds, however, were hypothetically identified as plant metabolites. Therefore, the study suggested that antioxidant activity exhibited by P. maculata extracts were due to non-enzymatic compounds such as uric acid and phenolic compounds originated from the animal’s metabolic activity and plants, respectively. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17390/1/49_05_12.pdf Khalida Khalil, and Syarul Nataqain Baharum, and Shazrul Fazry, and Nik Marzuki Sidik, and Fareed Sairi, (2020) Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract. Malaysian Applied Biology, 49 (5). pp. 115-124. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1078&catid=59:current-view&Itemid=56 |
| spellingShingle | Khalida Khalil, Syarul Nataqain Baharum, Shazrul Fazry, Nik Marzuki Sidik, Fareed Sairi, Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract |
| title | Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract |
| title_full | Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract |
| title_fullStr | Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract |
| title_full_unstemmed | Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract |
| title_short | Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract |
| title_sort | non-enzymatic antioxidant from apple snail (pomacea maculata) extract |
| url | http://journalarticle.ukm.my/17390/ http://journalarticle.ukm.my/17390/ http://journalarticle.ukm.my/17390/1/49_05_12.pdf |